Abstract
Nerolidol is a natural sesquiterpene alcohol with promising but limited application in food and pharmaceutical fields due to several factors including low photostability and low aqueous solubility. Recently, several carriers loading nerolidol were prepared and tested in fresh orange juice. Lipid vesicles loading nerolidol did not exhibit satisfactory organoleptic properties in this beverage. Hence, DMPC/DHPC bicelles were prepared as a new phospholipid-based carrier for nerolidol at different molar ratios. The bicelle suspensions were characterized in terms of homogeneity, particles size, and morphology. The optimal formulation (phospholipid:nerolidol molar ratio 100:1) was selected based on transparent appearance, homogeneity, and particle size (~ 45 nm). Besides, it showed a high encapsulation efficiency of nerolidol and a high incorporation rate of phospholipids. Transmission electron microscopy analysis demonstrated the formation of bicelles. The bicelles membrane fluidity was assessed by 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy and differential scanning calorimetry analysis. The membrane fluidity of bicelles appeared to increase in the presence of nerolidol in a concentration dependent manner. To our knowledge this is the first study dealing with the encapsulation of an essential oil component in bicelles.
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Abbreviations
- DLS:
-
Dynamic light scattering
- EE:
-
Encapsulation efficiency
- HPLC:
-
High performance liquid chromatography
- IR:
-
Incorporation rate
- Ner:
-
Nerolidol
- PdI:
-
Polydispersity index
- TEM:
-
Transmission electron microscopy
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Acknowledgements
The authors thank the Agence Universitaire de la Francophonie (Projet de Cooperation Scientifique Inter-Universitaire PCSI 2018-2020) and the Research Funding Program at the Lebanese University (project 2018/2020) for supporting this project.
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EE carried out the experiments and wrote the manuscript; HGG and CC supervised the work and edited the manuscript. AN edited the manuscript.
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Ephrem, E., Najjar, A., Charcosset, C. et al. Bicelles as a carrier for bioactive compounds in beverages: application to nerolidol, an active sesquiterpene alcohol. J Food Sci Technol 59, 1030–1039 (2022). https://doi.org/10.1007/s13197-021-05107-3
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DOI: https://doi.org/10.1007/s13197-021-05107-3