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Malabar sole (Cynoglossus macrostomus) skin as promising source of type I acid and pepsin solubilized collagens with potential bioactivity

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Abstract

Fish skin is one of the major non-edible by-products formed during fish processing. This investigation focused on the sustainable valorization of Malabar sole (MS) skin for collagen, which can be utilized as potential alternative of mammalian collagen. Acid and pepsin solubilized collagen (ASC and PSC) were successfully isolated from MS skin with a yield (%, dry weight basis) of 49.5 ± 0.6 and 67.6 ± 0.5, respectively. The isolated collagens were characterized by SDS-PAGE, UV-absorption, DSC, SEM, FTIR spectroscopy, etc., analysis. Both collagens were characterized as type I by SDS-PAGE and the well preserved triple helical structure by FTIR and UV absorption analysis. Denaturation temperature (°C) of the MS skin collagens confirmed by DSC analysis was 33.67 (ASC) and 33.38 (PSC). Both collagens showed high solubility in acidic pH and low NaCl level, and also exhibited a comparatively high degree of fibril-forming capacity. Antioxidant potential of the isolated collagens was confirmed by DPPH (31.4–34.6% at 1.5 mg) and peroxyl (64.6–68.3% at 0.3 mg) radical scavenging assays and observed a dose dependent manner activity. Overall, the results suggested the possibility of using the MS skin as a potential substitute source of realistic type I collagen and also help to reduce issues of fish processing discards.

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Acknowledgements

LCJ thank the University Grants Commission (UGC), New Delhi, India, for the award of a Research Fellowship. Authors thank the Director, CSIR-CFTRI, Mysuru, India for encouragement and facilities.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysuru, 570 020, India

    Lidiya C. Johny, M. Vijaykumar, Tanaji G. Kudre & P. V. Suresh

  2. Academy of Scientific and Innovative Research, Gazhiabad, India

    Lidiya C. Johny & P. V. Suresh

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  1. Lidiya C. Johny
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  2. M. Vijaykumar
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  3. Tanaji G. Kudre
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  4. P. V. Suresh
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Contributions

LCJ carried out the experiments; analyzed the data and drafted the manuscript; VM Performed the experiments and analyzed the data, TGK performed statistical analysis of the experimental data and edited the manuscript; SPV conceived and designed the study, provided resources, supervised, edited and reviewed manuscript.

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Correspondence to P. V. Suresh.

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Johny, L.C., Vijaykumar, M., Kudre, T.G. et al. Malabar sole (Cynoglossus macrostomus) skin as promising source of type I acid and pepsin solubilized collagens with potential bioactivity. J Food Sci Technol 59, 157–167 (2022). https://doi.org/10.1007/s13197-021-04996-8

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  • DOI: https://doi.org/10.1007/s13197-021-04996-8

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