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Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid

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Abstract

Porphyridium purpureum a red marine microalga is known for phycobiliproteins (PB), polyunsaturated fatty acids and sulphated exopolysaccharides. In the present study, effects of media constituents for the production of different polyunsaturated fatty acids from P. purpureum were considered using a response surface methodology (RSM). A second order polynomial was used to predict the response functions in terms of the independent variables such as the concentrations of sodium chloride, magnesium sulphate, sodium nitrate and potassium dihydrogen phosphate. The response functions were production of biomass yield, total lipid and polyunsaturated fatty acids like arachidonic acid (AA 20:4) and eicosapentaenoic acid (EPA 20:5). Results corroborated that maximum Biomass (0.95 gL−1) yield was at the concentrations of sodium chloride (14.89 gL−1), magnesium sulfate (3.93 gL−1) and sodium nitrate (0.96 gL−1) and potassium dihydrogen phosphate (0.09 gL−1). Optimum total lipid (17.9 % w/w) and EPA (34.6 % w/w) content was at the concentrations of sodium chloride (29.98 gL−1), magnesium sulfate (9.34 gL−1) and sodium nitrate (1.86 gL−1). Variation in concentration of potassium dihydrogen phosphate for both lipid (0.01gL−1) and EPA content (0.20 gL−1) was observed. The optimum conditions for biomass, total lipid, AA and EPA varied indicating their batch mode of growth and interaction effect of the salt.

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Acknowledgments

The authors thank Director, CSIR-CFTRI for for his encouragement and support. MDK acknowledge the ICMR, for research fellowship grant. Authors thank Dr. S. N. Mudliar for his critical evaluation of the manuscript. This paper was presented in International conference on “algal biorefinery: a potential source of food, feed, biochemicals, biofuels and biofertilizers”, 10-12th January, 2013, Kharagpur, India.

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  1. Shanmugam Kathiresan

    Present address: Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, India

Authors and Affiliations

  1. Plant Cell Biotechnology Department, Central Food, Mysore, 570020, India

    Mysore Doddaiah Kavitha & Ravi Sarada

  2. Grain Science Technology Department, Central Food Technological Research Institute, Mysore, 570020, India

    Sila Bhattacharya

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  1. Mysore Doddaiah Kavitha
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  3. Sila Bhattacharya
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Correspondence to Ravi Sarada.

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Kavitha, M.D., Kathiresan, S., Bhattacharya, S. et al. Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid. J Food Sci Technol 53, 2270–2278 (2016). https://doi.org/10.1007/s13197-016-2185-0

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  • DOI: https://doi.org/10.1007/s13197-016-2185-0

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