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Enzymatic hydrolysis of starry triggerfish (Abalistes stellaris) muscle using liver proteinase from albacore tuna (Thunnus alalunga)

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Abstract

Proteinases from liver extract from albacore tuna (Thunnus alalunga) were used to produce protein hydrolysate from starry triggerfish (Abalistes stellaris) muscle. Hydrolysis conditions for preparing protein hydrolysate from starry triggerfish muscle were optimized. Enzyme level, reaction time and fish muscle/buffer ratio significantly affected the hydrolysis (p < 0.05). Optimum conditions for triggerfish muscle hydrolysis were 5.5 % liver extract, 40 min reaction time and fish muscle/buffer ratio of 1:3 (w/v). The freeze-dried protein hydrolysate was characterized with respect to chemical composition, amino acid composition and color. The product contained 91.73 % protein, 2.04 % lipid and 6.48 % ash. The protein hydrolysate exhibited high amount of essential amino acids (45.62 %). It was light yellow in color (L * = 82.94, a * = 0.84, b * = 22.83). The results indicate that the extract from liver of albacore tuna could be used to produce fish protein hydrolysate and protein hydrolysate from starry triggerfish muscle may potentially serve as a good source of desirable peptide and amino acids.

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References

  • Adler-Nissen J (1986) Enzymatic hydrolysis of food proteins. Elsevier Spplied Science, London

    Google Scholar 

  • AOAC (2005) Official methods of analysis. Association of official analytical chemists international, 18th edn. AOAC International, Gaithersberg

    Google Scholar 

  • Benjakul S, Morrissey MT (1997) Protein hydrolysates from Pacific whiting solid wastes. J Agric Food Chem 45:3423–3430

    Article  CAS  Google Scholar 

  • Bhaskar N, Mahendrakar NS (2008) Protein hydrolysate from visceral waste proteins of Catla (Catla catla): optimization of hydrolysis condition for a commercial neutral protease. Bioresour Technol 99:4105–4111

    Article  CAS  Google Scholar 

  • Capiralla H, Hiroi T, Hirokawa T, Maeda S (2002) Purification and characterization of a hydrophobic amino acid-specific endopeptidase from Halobacterium halobium S9 with potential application in debittering of protein hydrolysates. Process Biochem 38:571–579

    Article  CAS  Google Scholar 

  • Chalamaiah M, Narsing Rao G, Rao DG, Jyothirmayi T (2010) Protein hydrolysates from meriga (cirrhinus mrigala) egg and evaluation of their functional properties. Food Chem 120:652–657

    Article  CAS  Google Scholar 

  • Chalamaiah M, Dinesh Kumar B, Hemalatha R, Jyothirmayi T (2012) Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: a review. Food Chem 135:3020–3038

    Article  CAS  Google Scholar 

  • Choi YJ, Hur S, Choi BD, Konno K, Park JW (2009) Enzymatic hydrolysis of recovered protein from frozen small croaker and functional properties of its hydrolysates. J Food Sci 74:C17–C24

    Article  CAS  Google Scholar 

  • Cohen SA, Michaud DP (1993) Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography. Anal Biochem 211:279–287

    Article  CAS  Google Scholar 

  • Guerard F, Dufosse L, De La Broise D, Binet A (2001) Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacores) wastes using Alcalase. J Mol Catal B 11:1051–1059

    Article  CAS  Google Scholar 

  • Hevia P, Olcott HS (1977) Flavor of enzyme-solubilized fish protein concentrate fractions. J Agric Food Chem 25:772–775

    Article  CAS  Google Scholar 

  • Khantaphant S, Benjakula S, Kishimura H (2011) Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases. Process Biochem 46:318–327

    Article  CAS  Google Scholar 

  • Klomklao S, Benjakul S, Visessanguan W, Simpson BK, Kishimura H (2005) Partitioning and recovery of proteinase from tuna spleen by aqueous two-phase systems. Process Biochem 40:3061–3067

    Article  CAS  Google Scholar 

  • Klomklao S, Kishimura H, Yabe M, Benjakul S (2007) Purification and characterization of two pepsins from the stomach of pectoral rattail (Coryphaenoides pectoralis). Comp Biochem Physiol 147B:682–689

    Article  CAS  Google Scholar 

  • Klomklao S, Kishimura H, Benjakul S (2013) Use of viscera extract from hybrid catfish (Clarias macracephalus × Clarias gariepinus) for the production of protein hydrolysate from toothed ponyfish (Gazza minuta) muscle. Food Chem 136:1006–1012

    Article  CAS  Google Scholar 

  • Kristinsson HG, Rasco BA (2000) Fish protein hydrolysates: production, biochemical, and functional properties. Crit Rev Food Sci Nutr 40:43–81

    Article  CAS  Google Scholar 

  • Liaset B, Lied E, Espe M (2000) Enzymatic hydrolysis of by-products from the fish-filleting industry; chemical characterization and nutritional evaluation. J Sci Food Agric 80:581–589

    Article  CAS  Google Scholar 

  • Morr V, German B, Kinsella JE, Regenstein JM, Van Buren JP, Kilara A, Lewis BA, Mangino ME (1985) A collaborative study to develop a standardized food protein solubility procedure. J Food Sci 50:1715–1718

    Article  CAS  Google Scholar 

  • Nalinanon S, Benjakul S, Visessanguan W, Kishimura H (2008) Partitioning of protease from stomach of albacore tuna (Thunnus alalunga) by aqueous two-phase systems. Process Biochem 44:471–476

    Article  Google Scholar 

  • Neklyudov AD, Ivankin AN, Berdutina AV (2000) Properties and uses of protein hydrolysates. Appl Biochem Microbiol 36:452–459

    Article  Google Scholar 

  • Nielsen PM (1997) Functionality of protein hydrolysates. In: Damodaran S, Paraf A (eds) Food proteins and their applications. Marcel Dekker, New York

    Google Scholar 

  • Ovissipour M, Abedian A, Motamedzadegan A, Rasco B, Safari R, Shahidi H (2009) The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysates from Persian sturgeon (Acipenser persicus) viscera. Food Chem 115:238–242

    Article  CAS  Google Scholar 

  • Ovissipour M, Benjakul S, Safari R, Motamedzagan A (2010) Fish protein hydrolysates production from yellowfin tuna Thunnus albacores head using Alcalase and Protamex. Int Aquat Res 2:87–95

    Google Scholar 

  • Phanturat P, Benjakul S, Visessanguan W, Roytrakul S (2010) Use of pyloric caeca extract from bigeye snapper (Priacanthus macracanthus) for the production of gelatin hydrolysate with antioxidative activity. LWT Food Sci Technol 43:86–97

    Article  CAS  Google Scholar 

  • Sathivel S, Bechtel PJ, Babbitt J, Smiley S, Crapo C, Reppond KD, Prinyawiwatkul W (2003) Biochemical and functional properties of herring (Clupea harengus) byproduct hydrolysate. J Food Sci 68:2196–2200

  • Senphan T, Benjakul S (2014) Use of the combined phase partitioning systems for recovery of proteases from hepatopancreas of Pacific white shrimp. Sep Purif Technol 129:57–63

    Article  CAS  Google Scholar 

  • Shahidi F (1994) Proteins from seafood processing discards. In: Sikorski ZE, Pan BS, Shahidi F (eds) Seafood proteins. Chapman & Hall, New York

    Google Scholar 

  • Shahidi F, Xiao-Qing H, Synowiecki J (1995) Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem 53:285–293

    Article  CAS  Google Scholar 

  • Sripokar P, Poonsin T, Chaijan M, Benjakul S, Klomklao S (2015) Proteinases from liver of albacore tuna (Thunnus alalunga): optimum extractant and biochemical characteristics. J Food Biochem. Accepted

  • Steel RGD, Torrie JH (1980) Principles and procedures of statistics: a biometrical approach. McGraw-Hill, New York

    Google Scholar 

  • Surowka K, Fik M (1994) Studied on the recovery of proteinaceous substrates from chicken heads: II-application of pepsin to the production of protein hydrolysate. J Sci Food Agric 65:289–286

    Article  CAS  Google Scholar 

  • Thiansilakul Y, Benjakul S, Shahidi F (2007) Antioxidant activity of protein hydrolysate from round scad muscle using Alcalase. J Food Biochem 31:266–287

    Article  CAS  Google Scholar 

  • Twasaki M, Harada R (1985) Proximate and amino acid composition of the roe and muscle of selected marine species. J Food Sci 50:1585–1587

    Article  Google Scholar 

  • Wisuthipheat N, Kongruang S (2015) Production of fish protein hydrolysates by acid and enzymatic hydrolysis. J Med Bioeng 4:466–470

    Google Scholar 

  • Wu HC, Chen HM, Shiau CY (2003) Free amino acid and peptide as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 36:949–957

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Thailand Research Fund and Thaksin University. Authors would like to thank the Office of the Higher Education Commission for financial support. The TRF distinguished research professor grant was also acknowledged.

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Authors and Affiliations

  1. Biotechnology Program, Faculty of Technology and Community Development, Phatthalung Campus, Thaksin University, Phatthalung, 93210, Thailand

    P. Sripokar

  2. Department of Food Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, 80160, Thailand

    M. Chaijan

  3. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    S. Benjakul

  4. Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    H. Kishimura

  5. Department of Food Science and Technology, Faculty of Technology and Community Development, Phatthalung Campus, Thaksin University, Phatthalung, 93210, Thailand

    S. Klomklao

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  1. P. Sripokar
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  2. M. Chaijan
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  3. S. Benjakul
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  4. H. Kishimura
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  5. S. Klomklao
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Correspondence to S. Klomklao.

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Sripokar, P., Chaijan, M., Benjakul, S. et al. Enzymatic hydrolysis of starry triggerfish (Abalistes stellaris) muscle using liver proteinase from albacore tuna (Thunnus alalunga). J Food Sci Technol 53, 1047–1054 (2016). https://doi.org/10.1007/s13197-015-2138-z

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  • DOI: https://doi.org/10.1007/s13197-015-2138-z

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