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2β-hydroxybetulinic acid 3β-caprylate: an active principle from Euryale Ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats

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Abstract

The aim of the present study was to evaluate the glycemic control, antioxidant, pancreas and liver protective effect of 2β-hydroxybetulinic acid 3β-caprylate (HBAC) from Euryale ferox Salisb. seeds on streptozotocin induced diabetic rats. The active principle was isolated from Euryale ferox Salisb. seeds extract by utilizing chromatographic techniques. The rats were divided into seven experimental groups: Gp 1-normal; Gp2- normal + HBAC (60 mg/kg p.o.); Gp3- diabetic control; Gp 4- Diabetic + HBAC (20 mg/kg p.o.); Gp5- Diabetic + HBAC (40 mg/kg p.o.); Gp6- Diabetic + HBAC (60 mg/kg p.o.) and Gp 7- Diabetic + Glibenclamide (10 mg/kg p.o.). Biochemical estimation, free radical scavenging examination and histopathological study was performed at the end of experimentation i.e. on 28th day. The active principle isolated and identified with spectral data as 2β-hydroxybetulinic acid 3β-caprylate (HBAC). It was detected for the first time that HBAC has improvised the glycemic control in streptozotocin induced diabetic rats. Furthermore, it is remarkable to note that it exhibited excellent free radical scavenging property and pancreas and hepatoprotective property as well, supported by histopathological examination. One of the mechanisms of action of HBAC appears to be stimulating the release of insulin from pancreatic β-cells. HBAC improved the glycemic control, reduced the free radical activity along with corrected glycemic control, lipid profile, and enhanced level of insulin alongh with improvement in pancreas and hepatoprotective architecture. Considering the above results, HBAC shows potential to develop a medicine for diabetes as combinatorial or mono-therapy.

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Acknowledgments

Authors are thankful to the authorities of Sam Higginbottom Institute of Agriculture, Technology and Sciences for providing necessary facilities. Authors are also thankful to the SAIF, Chandigarh for spectral data analysis.

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Authors and Affiliations

  1. Deparment of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institutue of Agriculture, Technology & Sciences (SHIATS)-Deemed University, Allahabad, Uttar Pradesh, India

    Danish Ahmed, Vikas Kumar & Amita Verma

  2. Department of Medical Laboratory & Technology, Faculty of Health Sciences, Sam Higginbottom Institutue of Agriculture, Technology & Sciences (SHIATS)-Deemed University, Allahabad, Uttar Pradesh, India

    Harish Kumar Bajaj

  3. Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

    Manju Sharma

  4. Hamdard Institute of Medical Sciences & Research (HIMSAR), Jamia Hamdard, New Delhi, India

    Manju Sharma

Authors
  1. Danish Ahmed
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  2. Manju Sharma
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  3. Vikas Kumar
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  4. Harish Kumar Bajaj
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  5. Amita Verma
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Corresponding authors

Correspondence to Danish Ahmed or Manju Sharma.

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Research highlights

1. Isolation of compounds from Euryale ferox salisb. were done.

2. Antidiabetic & antioxidant activities were investigated.

3. Histopathological examinations were envisaged.

4. Possible mechanism of antidiabetic action of isolated compounds were examined

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Ahmed, D., Sharma, M., Kumar, V. et al. 2β-hydroxybetulinic acid 3β-caprylate: an active principle from Euryale Ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats. J Food Sci Technol 52, 5427–5441 (2015). https://doi.org/10.1007/s13197-014-1676-0

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  • DOI: https://doi.org/10.1007/s13197-014-1676-0

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