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Experimental Validation of Antidiabetic and Antioxidant Potential of Cassia tora (L.): An Indigenous Medicinal Plant

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Abstract

The present study was undertaken to evaluate antidiabetic and antioxidant activities of Cassia tora (C. tora) seeds extract against streptozotocin induced diabetes in experimental rats to scientifically validate its use against diabetes. Ethanolic extract of C. tora seeds extract and standard drug (glibenclamide) prepared in aqueous gum acacia (2 %, w/v) suspension and fed orally to streptozotocin induced male adult diabetic rats of Charles Foster strain for 15 days. Biochemical parameters in normal, diabetic control, standard (600 μg/kg bw p.o.) and treated (500 mg/kg bw p.o.) animal groups were quantified and compared. Treatment of streptozotocin induced diabetic rats with ethanolic seeds extract caused significant (p < 0.001) reduction in blood glucose (270–220 mg/dl), total cholesterol (140–104 mg/dl), triglyceride (149–99 mg/dl), phospholipids (100–74 mg/dl), free fatty acid (2.39–2.00 μmol/l), lipid peroxide (9–5.63 nmol MDA/dl) and significantly increased post heparin lipolytic activity (11–14 nmol FFA released/h/l plasma) (p < 0.001). Furthermore, the seeds extract (100–400 μg) when tested for its antioxidant activity in vitro, showed significant (p < 0.001) inhibition in the generation of super oxide anions in enzymic system a (46–37, 33, 23, 21 nmol uric acid formed/min), in enzymic system b (113–91, 77, 60, 51 nmol formazon formed/min), non-enzymic system (324–230, 211, 161, 141 nmol uric acid formed/min) and hydroxyl radicals in enzymic system (544–501, 411, 319, 291 nmol 2,3-dihydroxybenzoate formed/h) and non-enzymic system (28–21, 17, 14, 12). The results of the present study demonstrated antidiabetic, antidyslipidemic and antioxidant activities of C. tora seeds which could help in prevention of diabeticdyslipidemia and related complications.

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Acknowledgments

One of us (Vishnu Kumar) is grateful to the Director, Central Drug Research Institute (CDRI), Lucknow for experimental support, Director Academics, Era’s Lucknow Medical College and Hospital, Lucknow for financial support and Late Dr. Ramesh Chander retired Scientist, Biochemistry, Division, CDRI, Lucknow for his expert guidance.

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Authors and Affiliations

  1. Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Sarfarajganj, Hardoi Road, Lucknow, 226 003, India

    Vishnu Kumar & Farzana Mahdi

  2. Department of Biochemistry, King George’s Medical University, Lucknow, 226 003, India

    Ranjana Singh & Abbas Ali Mahdi

  3. Department of Biochemistry, T S Misra Medical College and Hospital, Opposite Amausi Railway Station, Lucknow, 226 008, India

    Raj Kumar Singh

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  1. Vishnu Kumar
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  2. Ranjana Singh
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  4. Abbas Ali Mahdi
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Correspondence to Raj Kumar Singh.

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The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants performed by any of the authors. The study was approved by the Institutional Animal Ethics Committee of Central Drug Research Institute and was carried out in accordance with the current guidelines set by Organization for Economic Co-operation and Development (OECD), received from Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Social Justice and Empowerment, Government of India for the care of laboratory animals.

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Kumar, V., Singh, R., Mahdi, F. et al. Experimental Validation of Antidiabetic and Antioxidant Potential of Cassia tora (L.): An Indigenous Medicinal Plant. Ind J Clin Biochem 32, 323–328 (2017). https://doi.org/10.1007/s12291-016-0608-3

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  • DOI: https://doi.org/10.1007/s12291-016-0608-3

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