Abstract
The present study envisages the influence of essential oils namely lemon grass, eucalyptus, clove and neem on Kinnow mandarin with the objective to combat major post harvest diseases and to prolong its availability for longer time in the season. For this, in vitro and in vivo studies were conducted. Poisoned food technique was used for in vitro studies, and for in vivo studies, Kinnow fruit were pre-inoculated with pathogens (Penicillium digitatum and P. italicum), treated with different essential oils and then stored at 5 °C ±1 °C temperature and 85–90 % RH). Our results indicated that all essential oils inhibited the growth (colony diameter) of both pathogens over untreated PDA plates, but the inhibition was the strongest by lemon grass oil. Similarly, under in vivo conditions, all essential oils influenced decay incidence, decay loss, lesion diameter, respiration rate, ethylene evolution, overall acceptability and physiological loss in weight but lemon grass was the most effective. And also the incidence of Penicillium italicum was more noticed in fruits than P. digitatum, however, it was reverse under in vitro conditions. The decay rot at all stages of storage was less in EOs treated fruits than untreated fruits, thereby increasing their storage life significantly. Thus, it is evident from our studies that essential oils have the potential to control green and blue mold without causing any injury or harmful effects on Kinnow mandarin, and EOs can be recommended as a safe method for extending its storage life while maintaining fruit quality.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13197-014-1281-2/MediaObjects/13197_2014_1281_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13197-014-1281-2/MediaObjects/13197_2014_1281_Fig2_HTML.gif)
Similar content being viewed by others
References
Aggarwal KK, Khanuja SPS, Ahmad A, Kumar TRS, Gupta VK, Kumar S (2002) Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicita and Anethum sowa. Flavour Frag J 17:59–63
Amiri A, Dugas R, Pichot AL, Bompeix G (2008) In vitro and in vitro activity of eugenol oil (Eugenia caryophylata) against four important postharvest apple pathogens. Intl J Food Microbiol 126:13–19
Anthony S, Abeywickrama K, Wigaratnam SW (2003) The effect of spraying essential oils Cymbopagon nardus, Cymbopagon flexuosus and Ocimum basilicum on postharvest diseases and storage life of embul banana. J Hortic Sci Biotechnol 78:780–785
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475
Bhardwaj SS, Sharma M (1999) Diseases of minor fruits. In: Verma LR, Sharma RC (eds) Diseases of Horticultural crops-fruits. Indus Publishing co, New Delhi, pp 540–562
Brown GE, Eckert JW (2000) Penicillium decays. In: Timmer LW, Gamsey SM, Graham JH (eds) Compendium of citrus diseases, 2nd edn. APS, St Paul, pp 41–42
Cristescu SM, De Martinis D, Hekkert SL, Parker DH, Harren FJM (2002) Ethylene production by Botrytis cinerea in vitro and in tomatoes. Appl Environ Microbiol 68:5342–5350
Dixit SN, Chandra H, Tiwari R, Dixit V (1995) Development of a botanical fungicide against blue mold of mandarins. J Stored Products Res 31:165–172
Eckert JW, Eaks IL (1989) Postharvest disorders and diseases of citrus fruits. In: Renter W, Calavan GE (eds) The citrus industry, vol 5, University of California Press. University of California Press, Berkeley, pp 179–260
Eckert JW, Brown GE (1986) Post harvest citrus disease and their control. In: Fresh citrus fruits. AVI publishing Co., Westport, pp 315–360
Golam-Rabbany ABM, Mizutani F (1996) Effect of essential oils on ethylene production and ACC content in apple fruit and peach seed tissues. J Jpn Soc Hort Sci 65:7–13
Ismail M, Zhang J (2004) Post-harvest citrus diseases and their control. Outlook Pest Manag 15:29–35
Jhalegar MJ, Sharma RR, Pal RK, Rana V (2012) Effect of postharvest treatments with polyamines on physiological and biochemical attributes of kiwifruit (Actinidia deliciosa) cv Allison. Fruits 67:13–22
Kaur P, Verma KS (2002) Prevalence of post harvest rots of kinnow in Punjab. Pl Dis Res 17(2):329–331
Martinez-Romero D, Guillén F, Valverde JM, Bailén G, Zapata PJ, Serrano M, Castillo S, Valero D (2007) Influence of carvacrol on survival of Botrytis cinerea inoculated in table grapes. Intl J Food Microbiol 115:144–148
Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithanasan V, Parker M (2006) Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Intl J Food Microbiol 107(2):180–185
Moline HE, Locke JC (1993) Comparing Neem seed oil with calcium chloride and fungicides for controlling postharvest apple decay. Hortsci 28(7):719–720
Neri F, Mari M, Brigati S (2006) Control of Penicillium expansum by plant volatile compounds. Plant Pathol 55:100–105
Panse VG, Sukhatme PV (1984) Statistical methods for agricultural workers. ICAR, New Delhi, pp 288–290
Paster N, Bullerman LB (1988) Mold spoilage and mycotoxin formation in grains as controlled by physical means. Intl J Food Microbiol 7:257–265
Paviani L, Pergher SBC, Dariva C (2006) Application of molecular sieves in the fractionation of lemongrass oil from high-pressure carbon dioxide extraction. Braz J Chem Eng 23:219–225
Pezo D, Salafranca J, Nerin C (2006) Design of a method for generation of gas-phase hydroxyl radicals, and use of HPLC with fluorescence detection to assess the antioxidant capacity of natural essential oils. Anal Bioanal Chem 385(7):1241–1246
Plaza P, Usall J, Torres R, Lamarca N, Ascensio A, Vinas I (2003) Control of green and blue mold by curing on oranges during ambient and cold storage. Postharv Biol Technol 28:195–198
Porat R, Daus A, Weiss B, Cohen L, Droby S (2002) Effects of combing hot water, sodium bicarbonate and biocontrol on postharvest decay of citrus fruit. J Hortic Sci Biotechnol 77:441–445
Rabiei V, Shirzadeh E, RabbiAngourani H, Sharafi Y (2011) Effect of thyme and lavender essential oils on the qualitative and quantitative traits and storage life of apple ‘Jonagold’ cultivar. J Medicinal Plants Res 5(23):5522-5527
Ranganna S (1999) Handbook of Analysis and Quality Control for Fruit and Vegetable Products, 2nd edn. Tata McGraw-Hill Publishing Company Ltd., New Delhi
Samra NR, Mansour AM, Tourky MN, Tarbih ME (2006) Pre and post harvest treatments on peach fruit grown under desert conditions. J Agric Sci Mansoura Univ 31:7835–7846
Serrano M, Martinez- Romero D, Castillo S, Guillen D, Valero M (2005) The use of the natural antifungal compounds improves the beneficial effect of MAP in sweet cherry storage. Innov Food Emerging Technol 6:115–123
Sharma RR, Saxena SK (2004) Rootstocks influence granulation in Kinnow (C. nobilis × C. deliciosa). Sci Hortic 101:235–242
Sharma S, Sharma RR, Pal RK, Jhalegar MJ, Singh J, Srivastav M, Dhiman MR (2012) Ethylene absorbents influence fruit firmness and activity of enzymes involved in fruit softening of Japanese plum (Prunus salicina Lindell) cv Santa Rosa. Fruits 67:257–266
Sivakumar D, Hewarathgamagae NK, Wijeratnam RSW, Wijesundera RLC (2002) Effect of ammonium carbonate and sodium bicarbonate on anthracnose of papaya. Phytoparasitica 30:1–7
Tian J, Ban XQ, Zeng H, He JS, Huang B, Wang YW (2011) Chemical composition and antifungal activity of essential oil from Cicuta virosa L. var. latisecta Celak. Int J Food Microbiol 145:464–470
Tripathi P, Dubey NK, Shukla AK (2008) Use of some essential oils as postharvest botanical fungicides in the management of gray moulds of grapes caused by Botrytis cinerea. World J Microbial Biotechnol 24:39–46
Valero M, Giner MJ (2006) Effects of antimicrobial components of essential oils on growth of Bacillus cereus INRA L2104 in and the sensory qualities of carrot broth. Intl J Food Microbiol 106(1):90–94
Wisniewski M, Wilson C, El-Ghaouth A, Droby S (2001) Non chemical approaches to postharvest disease control. Acta Hortic 553:407–412
Yahyazadeh M, Omidbaigi R, Zare R, Taheri H (2008) Effect of some essential oils on mycelial growth of Penicillium Sacc. World J Microbial Biotechnol 24:1445–1450
Acknowledgments
We thank Dr. V.R. Sagar, Head, Division of Post Harvest Technology, IARI, New Delhi for providing all lab facilities. We are also thankful to Dr. Madan Pal Singh, Division of Plant Physiology, IARI, New Delhi for valuable advice during this investigation. The financial support of the ICAR in the form of Senior Research Fellow is duly acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jhalegar, M.J., Sharma, R.R. & Singh, D. In vitro and in vivo activity of essential oils against major postharvest pathogens of Kinnow (Citrus nobilis × C. deliciosa) mandarin. J Food Sci Technol 52, 2229–2237 (2015). https://doi.org/10.1007/s13197-014-1281-2
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-014-1281-2