Abstract
Fructooligosaccharide (FOS), a prebiotic was extracted from the grain of Coix lachryma-jobi Linn. (Job’s tears) by hot water extraction at 60 °C for 1 h. The resulting dried powder extract was assayed for FOS content of 1-kestose (GF2), nystose (GF3) and 1-β-D-fructofuranosylnystose (GF4) using HPLC equipped with RI detector. Total FOS content of the extract was 24.98 ± 7.48% (g/100 g crude extract). The biological activity including antioxidant and cytotoxicity of the FOS-containing extract was determined. The antioxidant activity by DPPH free radical scavenging of FOS-containing extract was comparable to vitamin C (0.97 fold of vitamin C) with a slight lipid peroxidation inhibition activity. The extract exhibited no cytotoxic effect on normal human skin fibroblast. These results have confirmed not only the source of FOS from Job’s tears extract but also its potential application as antioxidant in food or cosmetic products.
Similar content being viewed by others
References
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. J Food Sci Technol 28(1):25–30
Bunce TJ, Howard MD, Allee GL, Pace LW (1995) Protective effects of fructooligosaccharide (FOS) in prevention of mortality and morbidity from infectious E. coli K:88 challenge. J Anim Sci 73(suppl 1):69 (Abstr.)
Choi KH, Namhrng H, Paid IK (1994) Effects of dietary fructo-oligosaccharide on the suppression of intestinal colonization of Salmonella typhimurium in broiler chickens. Korean J Anim Sci 36:271–284
Dechsakulthorn F, Hayes A, Bakand S, Joeng L, Winder C (2007) In vitro cytotoxicity assessment of selected nanoparticles using human skin fibroblasts. Proc 6th World Congress on Alternatives & Animal Use in the Life Sciences 14:21–25
Decker EA, Welch B (1990) Role of ferritin as a lipid oxidation catalyst in muscle food. J Agr Food Chem 38:674–677
Fukata T, Sasai K, Miyamoto T, Baba E (1999) Inhibitory effects of competitive exclusion and fructooligosaccharide, singly and in combination, on Salmonella colonization of chicks. J Food Prot 62:229–233
Hidaka H, Eida T, Hanaya T (1986) Livestock feed containing inulo-oligosaccharides and breeding of livestock by using the same. European Patent No. 017026A2
Huebner J, Wehling RL, Parkhurst A, Hutkins RW (2008) Effect of processing conditions on the prebiotic activity of commercial prebiotics. Int Dairy J 18:287–293
Iordãchescu D, Dinu D, Bonoiu A, Bucatã G, Ciubãr R, Homeghiu C, Iancu C, Stan C, Nae S (2002) Time and dose-response study of the effects of vanadate on human skin fibroblasts. Romanian J Biophys 12:69–76
Jiang JW, Xiao QX (1986) Handbook of active ingredients from medicinal plants. People’s Health Press, Beijing
Jideani IA, Jideani VA (2011) Developments on the cereal grains Digitaria exilis (acha) and Digitaria iburua (iburu). J Food Sci Technol 48:251–259
Kuo CC, Shih MC, Kuo YH, Chiang W (2001) Antagonism of free-radical-induced damage of adlay seed and its antiproliferative effect in human histolytic lymphoma U237 monocytic cells. J Agric Food Chem 49:1564–1570
Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS (2004) Antioxidative and antimutagenic effects of Korean Buckwheat, Sorghum, Millet and Job’s tears. J Korean Soc Food Sci Nutr 33(6):921–929
Lakkham K, Wangsomnuk P, Aromdee C (2009) Identification and quality of four varieties of adlay. Songklanakarin J Sci Technol 31(4):425–431
Lee JK, Kim DB, Kim JI, Kim PY (2000) In vitro cytotoxicity tests on cultured human skin fibroblasts to predict skin irritation potential of surfactants. Toxicol in Vitro 14(4):345–349
Li DP (2006) Research advance on ethenopharmacology, pharmacodynamics, pharmacokinetics and clinical therapeutics of Coix seed and its preparation, Kanglaite injection. Asian J Pharmacodyn Pharmacokinet 6(2):83–102
Manosroi A, Ruksiriwanich W, Abe M, Sakai H, Manosroi W, Manosroi J (2010) Biological activities of the rice bran extract and physical characteristics of its entrapment in niosomes by supercritical carbon dioxide fluid. J Supercritical Fluids 54:137–144
Manosroi A, Khositsuntiwong N, Komno C, Manosroi W, Werner RG, Manosroi J (2011) Chemical stability and cytotoxicity of human insulin loaded in cationic DPPC/CTA/DDAB liposomes. J Biomed Nanotechnol 7(2):308–316
Mesa MD, Silván JM, Olza J, Gil Á, Castillo MD (2008) Antioxidant properties of soy protein-fructooligosaccharide glycation systems and its hydrolysates. Food Res Int 41:606–615
Osawa T, Namiki M (1981) A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agr Biol Chem 45:735–739
Roberfroid MB, Vanloo JAE, Gibson GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 128:11–19
Sun Q, Shen H, Luo Y (2011) Antioxidant activity of hydrolysates and peptide fractions derived from porcine hemoglobin. J Food Sci Technol 48(1):53–60
Tachibana Y, Kikuzaki H, Hj-Lajis N, Nakatani N (2001) Antioxidant activity of carbazoles from Murraya koenigii leaves. J Agr Food Chem 49:5589–5594
Tsai P, Hsieh YY, Huang TC (2004) Effect of sugar on anthocyanin degradation and water mobility in a roselle anthocyanin model system using 180 NMR. J Agr Food Chem 52:3097–3099
Tseng YH, Yang JH, Chang HL, Lee YL, Mau JL (2006) Antioxidant properties of methanolic extracts from monascal adlay. Food Chem 97:375–381
Xu ZR, Hu CH, Wang MQ (2002) Effects of fructooligosaccharide on conversion of L-tryptophan to skatole and indole by mixed populations of pig fecal bacteria. J Gen Appl Microbiol 48:83–89
Xu ZR, Hu CH, Zhan XA, Wang MQ (2003) Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Sci 82:1030–1036
Acknowledgement
This work was supported by the Thailand Research Fund (TRF) and Natural Product Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University (Chiang Mai, Thailand).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Manosroi, J., Khositsuntiwong, N. & Manosroi, A. Biological activities of fructooligosaccharide (FOS)-containing Coix lachryma-jobi Linn. extract. J Food Sci Technol 51, 341–346 (2014). https://doi.org/10.1007/s13197-011-0498-6
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-011-0498-6