Skip to main content
Log in

Characteristics of freeze-dried fish protein isolated from saithe (Pollachius virens)

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Fish proteins isolated from by-products or low commercial/underutilized species using pH-shift process is a new source of proteins that may be used as wet or dried ingredients to develop value-added convenience foods. This paper reports the effects of freeze-drying on characteristics of fish protein isolates (FPI) from saithe (Pollachius virens) with or without lyoprotectants. Freeze-dried saithe mince from the same lot and without additives was used as a control. The resulting fish protein powders contained 71-93% protein, 1.5-3% moisture, 0-21% carbohydrate and 0.5-2% fat. Lipid oxidation (assessed by TBARS) of FPI powder groups was higher than that of fish mince powder. The results revealed that oxidation started during the pH-shift process and was increased by freeze-drying. Functional properties and sensory attributes were influenced by the advanced oxidation. However, the mince powder was less oxidized and had higher functional properties such as water binding capacity, gel forming ability, emulsification, foaming properties and colour and lower sensory scores for rancid odour and flavour than the FPI powders. Further studies on how to prevent oxidation of fish flesh during pH-shift and drying processes are recommended.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Washington DC

    Google Scholar 

  • APHA (1992) Compendium of methods for the microbiological examination of foods. American Public Health Association (APHA), Vanderzant C and Splittstoesser DF, (eds), Washingthon DC

  • Belitz HD, Grosch W, Schieberle P (2009) Food chemistry, 4th edn. Springer, Berlin, pp 211–218

    Google Scholar 

  • Bragadottir M, Reynisson E, Thorarinsdottir KA, Arason S (2007) Stability of fish powder made from saithe (Pollachinus virens) as measured by lipid oxidation and functional properties. J Aquat Food Prod Tech 16(1):115–136

    Article  CAS  Google Scholar 

  • Beuchat LR (1997) Functional and electrophoretic characteristics of succinylated peanut flour proteins. J Agric Food Chem 2:258–261

    Google Scholar 

  • Campo-Deanol L, Tovar CA, Borderias J (2010) Effects of several cryoprotectants on the physicochemical and rheological properties of suwari gels from frozen squid surimi by two methods. J Food Eng 97:457–464

    Google Scholar 

  • Carvajal PA, Lanier TC, MacDonald GA (2005) Stabilization of proteins in surimi. In: Park JW (ed) Surimi and Surimi seafood, 2nd edn. Francis and Taylor Group, USA, pp 202–220

    Google Scholar 

  • Choi YJ, Park JW (2002) Acid-aided protein recovery from enzyme-rich Pacific whiting. J Food Sci 8:2962–2967

    Article  Google Scholar 

  • Chung YC, Ho ML, Chyan FL, Jiang ST (2000) Utilization of freeze-dried mackerel (Scomber australasicus) muscle proteins as a binder in restructured meat. J Fish Sci 66:130–135

    Article  CAS  Google Scholar 

  • Cordova-Murueta JH, Navarrete Del Toro MA, Carreno FG (2007) Concentrates of fish protein from by-catch species produced by various drying processes. J Food Chem 100:705–711

    Article  CAS  Google Scholar 

  • Cortes-Ruis JA, Pacheco-Aguilar R, Garcia-Sanchez G, Lugo-Sanches ME (2001) Functional characterization of a protein concentrate from bristly sardine made under acidic conditions. J Aquat Food Prod Techn 4:5–23

    Article  Google Scholar 

  • Damodaran S (2008) Amino acids, peptids and proteins. In: Damodaran S (ed) Fennema's food chemistry, 4th edn. CRC Press, Taylor and Francis Group, New York, pp 120–150

    Google Scholar 

  • Eikevik TM, Stromment I, Alves-Filho O, Hemmingsen AKT (2005) Effect of operating conditions on atmospheric freeze-dried codfish. In: 3 rd Inter-American Drying Conference (IADC 2005), Montreal, 21-23 Aug, paper nr XIII-3

  • Fewtrell L, Pruss-Ustun A, Bos R, Gore F, Bartram J (2007) Water, sanitation and hygiene. World Health Organization, Geneva, pp p12–50

    Google Scholar 

  • Fontana AJ (1998) Water activity: why it is important for food safety, First International Conference on Food Safety. Nov. 16-18 1998. Albuquerque, NM. USA. Conference proceeding, p 177-185

  • Frankel EN (1998) Methods to determine extent of oxidation. In: Lipid Oxidation, Glasgow: Bell and Bain Ltd, p 79–98

  • Geirsdottir M (2005) Protein isolation from herring. Nordic Innovation Centre, Project No. 00075, p 102-103

  • Gill T (1995) Biochemical and chemical methods for assessment of fish quality. In: Huss HH (ed) Quality and quality changes in fresh fish. FAO Fisheries Technical Paper nr 348, Food and Organisation, Rome, pp 139–150

    Google Scholar 

  • Gutteridge JMC (1988) Lipid peroxidation: some problems and concepts. In: Halliwell B (ed) Oxygen radicals and tissue injury. Federation of American Societies of Experimental Biology, Bethesda, pp 9–19

    Google Scholar 

  • HAFRO (2010) State of marine stocks in Icelandic waters 2009/2010—Prospects for the Quota Year 2010/2011. Ministry of Fisheries and Agriculture, Marine Research Institute (HAFRO), Iceland

    Google Scholar 

  • Huda N, Aminah A, Babji AS (2000) Effects of cryoprotectants on functional properties of dried lizardfish (Saurida tumbil) surimi. Malays Appl Biol 29(1&2):9–16

    Google Scholar 

  • Huda N, Aminah A, Babji AS (2001) Functional properties of surimi powder from three Malaysian marine fish. Int J Food Sci Tech 36:401–406

    Article  CAS  Google Scholar 

  • Hultin HO, Kristinsson HG, Lanier TC, Park JW (2005) Process for recovery of functional proteins by pH shifts. In: Park JW (ed) Surimi and Surimi seafood. Taylor and Francis Group, Boca Raton, pp 107–139

    Chapter  Google Scholar 

  • ISO (1988) Sensory analysis-general guidance for the design of test rooms, International Organization for Standardization Geneva, Switzerland, Standard nr 8589

  • ISO (1993) Sensory analysis-general guidance for the selection, training and monitoring of assessors. Part 1: Selected assessors, International Organization for Standardization Geneva, Switzerland, Standard nr 8586-1

  • Jay JM (1992) Modern food microbiology, 4th edn. Chapman & Hall, New York, USA, pp 356–370

    Google Scholar 

  • Kilara A, Harwalkar VR (1996) Denaturation. In: Nakai S, Modler HW (eds) Food proteins properties and characterization. VCH, New York, pp 71–165

    Google Scholar 

  • Knipe CL (1992) Phosphates as meat emulsion stabilizers. From Phosphates as emulsifiers. Encyclopaedia of food science, food technology and nutrition. Academic Press Limited, London, pp 85–98

    Google Scholar 

  • Kristinsson HG, Demir N (2003) Functional fish protein ingredients from fish species of warm and temperate waters: Comparison of acid- and alkali-aided processing vs. conventional surimi processing. In: Advances in Seafood Byproducts. Bechtel PJ (ed), Conference Proceedings Anchorage: Alaska Sea Grant College Program University of Alaska, p 277–295

  • Kristinsson HG, Hultin HO (2004) The effect of acid and alkali unfolding and subsequent refolding on the pro-oxidative activity of trout hemoglobin. J Agric Food Chem 52:5482–5490

    Article  CAS  Google Scholar 

  • Kristinsson HG, Theodore AE, Demir N, Ingadottir B (2005) A comparative study between acid- and alkali-aided processing and surimi processing for the recovery of proteins from Channel catfish muscle. J Food Sci 70(4):298–306

    Article  Google Scholar 

  • Kristinsson HG, Liang Y (2006) Effect of pH-shift processing and surimi processing on Atlantic croaker (Micropogonias undulates) muscle proteins. J Food Sci 71(5):304–312

    Article  Google Scholar 

  • Lanier TC, Carvajal P (2005) Surimi gelation chemistry. In: Surimi and Surimi Seafood. Park, JW (ed), CRC Press, Taylor and Francis Group, p 435-489

  • Matsuda Y, Noguchi SF (1992) Cryostabilization of proteins in surimi. In: Lanier TC, Lee CM (eds) Surimi technology. Dekker, New York, pp 357–388

    Google Scholar 

  • Miller R, Groniger HS (1976) Functional properties of enzyme modified fish protein deviates. J Food Sci 41:268–272

    Article  CAS  Google Scholar 

  • Musa KH, Aminah A, Mustapha WAW, Ruslan MH (2002) Effect of solar drying on the functional properties of surimi. In: Conference, Asian Institute of Technology, Innovations in Food Processing Engineering and Technology, Bangkok, 11-13 December 2002, p 387-397

  • Musa KH, Aminah A, Wan-Aida WM (2005) Functional properties of surimi related to drying methods. Malays Appl Biol 34(2):83–87

    Google Scholar 

  • Olsen E, Aanes S, Mehl S, Holst JC, Aglen A, Gjoster H (2010) Cod, haddock, saithe, herring, and capelin in the Barents Sea and adjacent waters: a review of the biological value of the area. ICES J Mar Sci 67:87–101

    Article  Google Scholar 

  • Park JW (1995) Surimi gel colors as affected by moisture content and physical conditions. J Food Sci 60(1):15–18

    Article  CAS  Google Scholar 

  • Park JW (2005) Codex code for frozen Surimi. In: Park JW (ed) Surimi and Surimi seafood. Taylor and Francis Group, Boca Raton, pp 869–885

    Chapter  Google Scholar 

  • Perez-Mateos M, Lanier T (2006) Comparison of Atlantic menhaden gels from surimi processed by acid or alkaline solubilization. Food Chem 101:1223–1229

    Article  Google Scholar 

  • Ramirez JA, Diaz-Sobac R, Morales OG, Vazquez M (1999) Evaluation of freeze-dried surimi from tilapia and fat sleeper as emulsifier. Sci Technol Aliment 4:210–214

    Google Scholar 

  • Schenz TW, Morr CR (1996) Viscosity. In: Hall MG (ed) Methods of testing protein functionality. Blackie Academic and Professional Chapman and Hall, London, pp 62–73

    Google Scholar 

  • Shaviklo GR, Arason S, Thorkelsson G, Sveinsdottir K, Martinsdottir E (2010a) Sensory attributes of haddock balls affected by added fish protein isolate and frozen storage. J Sens Stud 25:316–331

    Article  Google Scholar 

  • Shaviklo GR, Thorkelsson G, Kristinsson HG, Arason S, Sveinsdottir K (2010b) The influence of additives and drying methods on quality attributes of fish protein powder made from saithe (Pollachius virens). J Sci Food Agric 90:2133–2143

    CAS  Google Scholar 

  • Shaviklo GR, Thorkelsson G, Arason S (2010c) The influence of additives and frozen storage on functional properties and flow behaviour of fish protein isolated from haddock (Melanogramms aeglefinus). Turk J Fish Aquat Sci 10:333–340

    Google Scholar 

  • Smith GM, Hardy R (2001) Handling and processing saithe. Torry Research Station, Torry Advisory Note nr 47

  • Tarladgis BG, Watts BM, Younathan MT (1960) A distillation method for the quantitative determination of malonaldehyde in rancid foods. JAOCS 37:44–48

    Article  CAS  Google Scholar 

  • Thawornchinsombut S, Park JW (2006) Frozen stability of fish protein isolate under various storage conditions. J Food Sci 71:227–30

    Article  Google Scholar 

  • Thorkelsson G, Sigurgisladottir S, Geirsdottir M, Johansson R, Guerad F, Chabeaud A, Bourseau P, Vandanjon L, Jaouen P, Chaplain-Derouiniot M, Fouchereau-Peron M, Martinez-Alvarez O, Le Gal Y, Ravallec-Ple R, Picot L, Berge JP, Delannoy C, Jakobsen G, Johansson I, Batista I, Pires C (2008) Mild processing techniques and development of functional marine protein and peptide ingredients. In: Improving seafood products for the consumer. Børresen T (ed), Woodhead Publishing Limited, p 363-386

  • Undeland I, Hall G, Wendin K, Gangby I, Rutgersson A (2005) Preventing lipid oxidation during recovery of functional proteins from herring (Clupea harengus) fillets by an acid solubilization process. J Agric Food Chem 14:5625–5634

    Article  Google Scholar 

  • Venugopal V, Chawla SP, Nair PM (1996) Spray-dried protein powder from Threadfin bream: preparation, properties and comparison with FPC type-B. J Muscle Food 7:55–71

    Article  Google Scholar 

  • Venugopal V, Shahidi F (1996) Structure and composition of fish muscle. Food Rev Int 2:175–197

    Article  Google Scholar 

  • Vojdani F (1996) Solubility. In: Hall GM (ed) Methods of testing protein functionality. Blackie Academic & Professional, London, pp 27–39

    Google Scholar 

  • Wilde PJ, Clark DC (1996) Foam formation and stability. In: Hall GM (ed) Methods for testing protein functionality. Blackie Academic & Professional, London, pp 110–148

    Chapter  Google Scholar 

  • WHO/FAO (2001) Recommended International Code of Practice General Principles of Food Hygiene. Food and Agriculture Organization of the United Nations and World Health Organization. Joint FAO/WHO Food Standards Programme, FAO, Rome

  • Yasumatsu K, Sawada K, Misaki SM, Toda J, Wada T, Ishi K (1972) Whipping and emulsifying properties. Agr Biol Chem 36:719–726

    Article  Google Scholar 

  • Yongsawatdigul J, Park JW (2004) Effects of alkali and acid solubilization on gelation characteristics of rockfish muscle proteins. J Food Sci 7:499–505

    Google Scholar 

Download references

Acknowledgements

Financing of the work by the United Nations University-Fisheries Training Program, Matis (Icelandic Food and Biotech R&D) and the Rannis (The Icelandic Centre for Research) is gratefully acknowledged. This study was also performed within the SEAFOODplus European Integrated Programme (http://www.seafoodplus.org) as part of the “Propephealth” project 4.1. The authors would like to thank Iceprotein for processing FPI and Adalheidur Olafsdottir and the sensory panel at Matis for their contribution.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gholam Reza Shaviklo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shaviklo, G.R., Thorkelsson, G., Arason, S. et al. Characteristics of freeze-dried fish protein isolated from saithe (Pollachius virens). J Food Sci Technol 49, 309–318 (2012). https://doi.org/10.1007/s13197-011-0285-4

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-011-0285-4

Keywords

Navigation