Abstract
Industrial waste molasses is one of the major soil pollutants. This waste increasingly affects land fertility, human health, and the environment. Hence, this study proposes an efficient method for the disposal of sugar cane residue and reutilization of value-added components from molasses juice. Algal strains (Oedogonium sp., Ulothrix sp., Cladophora sp., and Spirogyra sp.) are grown in several molasses concentrations. Their subsequent effect of molasses on algal growth, metabolite accumulation, lipid profiling, and biodiesel production are investigated for the first time. It was noticed that 0.5% molasses increase biomass production of all algal species. Compared to the control, the highest accumulation was 38% in Oedogonium sp. and 46% in Ulothrix sp. after 5 days. Total chlorophyll, carbohydrates, and protein were also increased in all species. Similarly, lipid content was increased from 21–43%, with suitable changes in the fatty acid profile C16 to C18 as a prerequisite for optimum biodiesel production. The efficacy of biodiesel was further verified by the biodiesel standards such as EN 14,214 and ASTM D6751 iodine value, saponification, cetane number, cold filter plugging point, density, kinematic viscosity, oxidative stability, long-chain saturation factor, and higher heating value are in the range of standards; all of these fuel properties were significantly improved in the molasses media. Molasses can stimulate algal species growth and metabolites synthesis, which ultimately produce higher lipid yield and better biofuel quality and quantity.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ANOVA:
-
Analysis of variance
- ASTM:
-
American Society for Testing and Materials
- CCD:
-
Central Composite Design
- CFPP:
-
Cold filter plugging point
- CN:
-
Cetane number
- C.V.:
-
Coefficient of Variation
- D:
-
Number of double bonds
- Di:
-
Number of double bonds in a given fatty acid
- DU:
-
Degree of unsaturation
- FAME:
-
Fatty acid methyl ester
- GCMS:
-
Gas chromatography-mass spectrometry
- HHV:
-
Higher heating value
- IV:
-
Iodine value
- KV:
-
Kinematic viscosity, υ
- LCSF:
-
Long chain saturated factor
- MUFA:
-
Mono unsaturated fatty acid
- Mwi:
-
Molecular weight of a fatty acid
- Ni:
-
Percentage of a given fatty acid
- PUFA:
-
Poly unsaturated fatty acid
- ROS:
-
Reactive Oxygen Species
- RSM:
-
Response surface methodology
- SV:
-
Saponification value
- Wt %:
-
Weight percentage
- ρ:
-
Density
References
Huang, J., Khan, M.T., Perecin, D., Coelho, S.T., Zhang, M.: Sugarcane for bioethanol production: potential of bagasse in Chinese perspective. Renew. Sustain. Energy Rev. 133, 110296 (2020)
Deseo, M.A., Elkins, A., Rochfort, S., Kitchen, B.: Antioxidant activity and polyphenol composition of sugarcane molasses extract. Food Chem. 314, 126180 (2020)
Asgher, M., Rani, A., Khalid, N., Qamar, S.A., Bilal, M.: Bioconversion of sugarcane molasses waste to high-value exopolysaccharides by engineered Bacillus licheniformis. Case Stud. Chem. Environ. Eng. 3, 100084 (2021)
L.T. Fuess, M. Zaiat, C.A.O. do Nascimento, Thermophilic biodigestion of fermented sugarcane molasses in high-rate structured-bed reactors: Alkalinization strategies define the operating limits, Energy Convers. Manag. 239 (2021) 114203.
Ikegami, K., Hirose, Y., Sakashita, H., Maruyama, R., Sugiyama, T.: Role of polyphenol in sugarcane molasses as a nutrient for hexavalent chromium bioremediation using bacteria. Chemosphere 250, 126267 (2020)
Oliveira, C.A., Fuess, L.T., Soares, L.A., Damianovic, M.H.R.Z.: Thermophilic biohydrogen production from sugarcane molasses under low pH: Metabolic and microbial aspects. Int. J. Hydrogen Energy. 45, 4182–4192 (2020)
A.D. Tripathi, R. Mishra, K.K. Maurya, R.B. Singh, D.W. Wilson, Estimates for world population and global food availability for global health, in: Role Funct. Food Secur. Glob. Heal., Elsevier, 2019: pp. 3–24.
Vilela, R.S., Fuess, L.T., Saia, F.T., Silveira, C.R.M., Oliveira, C.A., Andrade, P.A., Langenhoff, A., van der Zaan, B., Cop, F., Gregoracci, G.B.: Biofuel production from sugarcane molasses in thermophilic anaerobic structured-bed reactors. Renew. Sustain. Energy Rev. 144, 110974 (2021)
L.T. Fuess, L. Fuentes, P. Bovio-Winkler, F. Eng, C. Etchebehere, M. Zaiat, C.A.O. do Nascimento, Full details on continuous biohydrogen production from sugarcane molasses are unraveled: Performance optimization, self-regulation, metabolic correlations and quanti-qualitative biomass characterization, Chem. Eng. J. 414 (2021) 128934.
Ekstrom, J.A., Moore, S.K., Klinger, T.: Examining harmful algal blooms through a disaster risk management lens: A case study of the 2015 US West Coast domoic acid event. Harmful Algae 94, 101740 (2020)
N. Sharif, N. Munir, M. Hasnain, S. Naz, M. Arshad, Environmental Impacts of Ethanol Production System, in: Sustain. Ethanol Clim. Chang., Springer, 2021: pp. 205–223.
Munir, N., Abideen, Z., Sharif, N.: Development of halophytes as energy feedstock by applying genetic manipulations. All Life. 13, 1–10 (2020)
N. Munir, M. Hasnain, U. Roessner, Z. Abideen, Strategies in improving plant salinity resistance and use of salinity resistant plants for economic sustainability, Crit. Rev. Environ. Sci. Technol. (2021) 1–47.
Adewuyi, A.: Challenges and prospects of renewable energy in Nigeria: A case of bioethanol and biodiesel production. Energy Rep. 6, 77–88 (2020)
Hasnain, M., Abideen, Z., Naz, S., Roessner, U., Munir, N.: Biodiesel production from new algal sources using response surface methodology and microwave application. Biomass Convers. Biorefinery. (2021). https://doi.org/10.1007/s13399-021-01560-4
Saad, M.G., Selahi, A., Zoromba, M.S., Mekki, L., El-Bana, M., Dosoky, N.S., Nobles, D., Shafik, H.M.: A droplet-based gradient microfluidic to monitor and evaluate the growth of Chlorella vulgaris under different levels of nitrogen and temperatures. Algal Res. 44, 101657 (2019)
Khuram, I., Muhammad, Z., Ahmad, N., Ullah, R., Barinova, S.: Green and charophyte algae in bioindication of water quality of the shah alam river (District Peshawar, Pakistan), Transylvanian Rev. Syst. Ecol. Res. 21, 1–16 (2019)
Yanagawa, K., Haraguchi, A., Yoshitake, K., Asamatsu, K., Harano, M., Yamashita, K., Ishibashi, J.: Ubiquity of Euglena mutabilis Population in Three Ecologically Distinct Acidic Habitats in Southwestern Japan. Water 13, 1570 (2021)
A. Mayer, H. Tavakoli, C. Fessel Doan, A. Heidari, R. Handler, Modeling water‐energy tradeoffs for cultivating algae for biofuels in a semi‐arid region with fresh and brackish water supplies, Biofuels, Bioprod. Biorefining. 14 (2020) 1254–1269.
M. Hasnain, Z. Abideen, D. Anthony Dias, S. Naz, N. Munir, Utilization of Saline Water Enhances Lipid Accumulation in Green Microalgae for the Sustainable Production of Biodiesel, BioEnergy Res. (2022) 1–14.
N. Maheshwari, P.K. Krishna, I.S. Thakur, S. Srivastava, Biological fixation of carbon dioxide and biodiesel production using microalgae isolated from sewage waste water, Environ. Sci. Pollut. Res. (2019) 1–11.
Zhu, S., Wang, Y., Huang, W., Xu, J., Wang, Z., Xu, J., Yuan, Z.: Enhanced accumulation of carbohydrate and starch in Chlorella zofingiensis induced by nitrogen starvation. Appl. Biochem. Biotechnol. 174, 2435–2445 (2014)
Dawson, J.M., Heatlie, P.L.: Lowry method of protein quantification: evidence for photosensitivity. Anal. Biochem. 140, 391–393 (1984)
Lichtenthaler, H.K., Buschmann, C.: Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Curr. Protoc. Food Anal. Chem. 1, F4–F3 (2001)
Zhao, B., Zhang, S., Zhou, Y., He, D., Li, Y., Ren, M., Xu, Z., Fang, J.: Characterization and quantification of PAH atmospheric pollution from a large petrochemical complex in Guangzhou: GC–MS/MS analysis. Microchem. J. 119, 140–144 (2015)
Islam, M.A., Magnusson, M., Brown, R.J., Ayoko, G.A., Nabi, M., Heimann, K.: Microalgal species selection for biodiesel production based on fuel properties derived from fatty acid profiles. Energies 6, 5676–5702 (2013)
S. Ge, M. Madill, P. Champagne, Use of freshwater macroalgae Spirogyra sp. for the treatment of municipal wastewaters and biomass production for biofuel applications, Biomass and Bioenergy. 111 (2018) 213–223.
Piasecka, A., Krzemińska, I., Tys, J.: Enrichment of Parachlorella kessleri biomass with bioproducts: oil and protein by utilization of beet molasses. J. Appl. Phycol. 29, 1735–1743 (2017)
Piasecka, A., Nawrocka, A., Wiącek, D., Krzemińska, I.: Agro-industrial by-product in photoheterotrophic and mixotrophic culture of Tetradesmus obliquus: Production of ω3 and ω6 essential fatty acids with biotechnological importance. Sci. Rep. 10, 1–11 (2020)
Nagarajan, D., Kusmayadi, A., Yen, H.-W., Dong, C.-D., Lee, D.-J., Chang, J.-S.: Current advances in biological swine wastewater treatment using microalgae-based processes. Bioresour. Technol. 289, 121718 (2019)
Yew, G.Y., Puah, B.K., Chew, K.W., Teng, S.Y., Show, P.L., Nguyen, T.H.P.: Chlorella vulgaris FSP-E cultivation in waste molasses: Photo-to-property estimation by artificial intelligence. Chem. Eng. J. 402, 126230 (2020)
M.G. Tedesque, B. Scardoeli-Truzzi, L.H. Sipaúba-Tavares, Messastrum gracile (Chlorophyceae) growth using sugarcane molasses-based macrophyte extract culture media, J. Appl. Phycol. (2021) 1–10.
Liu, J., Huang, J., Jiang, Y., Chen, F.: Molasses-based growth and production of oil and astaxanthin by Chlorella zofingiensis. Bioresour. Technol. 107, 393–398 (2012)
El-Sheekh, M., Abomohra, A.E.-F., Hanelt, D.: Optimization of biomass and fatty acid productivity of Scenedesmus obliquus as a promising microalga for biodiesel production. World J. Microbiol. Biotechnol. 29, 915–922 (2013)
Yeesang, C., Cheirsilp, B.: Low-cost production of green microalga Botryococcus braunii biomass with high lipid content through mixotrophic and photoautotrophic cultivation. Appl. Biochem. Biotechnol. 174, 116–129 (2014)
El-Sheekh, M.M., Bedaiwy, M.Y., Osman, M.E., Ismail, M.M.: Influence of molasses on growth, biochemical composition and ethanol production of the green algae Chlorella vulgaris and Scenedesmus obliquus. J. Agric. Eng. Biotechnol. 2, 20 (2014)
George, B., Pancha, I., Desai, C., Chokshi, K., Paliwal, C., Ghosh, T., Mishra, S.: Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus–A potential strain for bio-fuel production. Bioresour. Technol. 171, 367–374 (2014)
N. Laraib, M. Manzoor, A. Javid, F. Jabeen, S.M. Bukhari, W. Ali, A. Hussain, Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: biomass productivity, lipid content, and fatty acid analyses, Environ. Prog. Sustain. Energy. (2021) e13625.
V.V. Devadas, K.S. Khoo, W.Y. Chia, K.W. Chew, H.S.H. Munawaroh, M.-K. Lam, J.-W. Lim, Y.-C. Ho, K.T. Lee, P.L. Show, Algae biopolymer towards sustainable circular economy, Bioresour. Technol. (2021) 124702.
I. Saidu, G.O. Abu, O. Akaranta, F.O. Chukwuma, S. Vijayalakshmi, J. Ranjitha, Biochemical Composition of Chlorella vulgaris Grown on Sugarcane Molasses, J. Eng. Res. Reports. (2021) 115–122.
Wang, Q., Wang, L.: Renewable energy consumption and economic growth in OECD countries: A nonlinear panel data analysis. Energy 207, 118200 (2020)
Fazelian, N., Movafeghi, A., Yousefzadi, M., Rahimzadeh, M., Zarei, M.: Impact of silver nanoparticles on the growth, fatty acid profile, and antioxidative response of Nannochloropsis oculata. Acta Physiol. Plant. 42, 1–14 (2020)
Diprat, A.B., Thys, R.C.S., Rodrigues, E., Rech, R.: Chlorella sorokiniana: A new alternative source of carotenoids and proteins for gluten-free bread. LWT. 134, 109974 (2020)
El-Sheekh, M.M., Gheda, S.F., El-Sayed, A.E.-K.B., Shady, A.M.A., El-Sheikh, M.E., Schagerl, M.: Outdoor cultivation of the green microalga Chlorella vulgaris under stress conditions as a feedstock for biofuel. Environ. Sci. Pollut. Res. 26, 18520–18532 (2019)
Dong, X., Huang, L., Li, T., Xu, J.-W., Zhao, P., Yu, X.: The enhanced biomass and lipid accumulation in algae with an integrated treatment strategy by waste molasses and Mg2+ addition, Energy Sources. Part A Recover. Util. Environ. Eff. 42, 1183–1192 (2020)
E. Yudiati, A. Djunaedi, D.S.K. Adziana, A.A. Nisa, R. Alghazeer, Improving Production, Chlorophyll a and Carotenoids Contents of Gracilaria sp. with Liquid Organic Fertilizer from Alginate Waste., Indones. J. Mar. Sci. Kelaut. 26 (2021).
Tan, X.-B., Meng, J., Tang, Z., Yang, L.-B., Zhang, W.-W.: Optimization of algae mixotrophic culture for nutrients recycling and biomass/lipids production in anaerobically digested waste sludge by various organic acids addition. Chemosphere 244, 125509 (2020)
B. Turkkul, O. Deliismail, E. Seker, Ethyl esters biodiesel production from Spirulina sp. and Nannochloropsis oculata microalgal lipids over alumina-calcium oxide catalyst, Renew. Energy. 145 (2020) 1014–1019.
Hossain, S.M.Z., Razzak, S.A., Al-Shater, A.F., Moniruzzaman, M., Hossain, M.M.: Recent advances in enzymatic conversion of microalgal lipids into biodiesel. Energy Fuels 34, 6735–6750 (2020)
M.L. Menegazzo, H.B. Ulusoy-Erol, C.N. Hestekin, J.A. Hestekin, G.G. Fonseca, Evaluation of the yield, productivity, and composition of fatty acids methyl esters (FAME) obtained from the lipidic fractions extracted from Chlorella sorokiniana by using ultrasound and agitation combined with solvents, Biofuels. (2020) 1–8.
Nguyen, Q.-H., Talou, T., Evon, P., Cerny, M., Merah, O.: Fatty acid composition and oil content during coriander fruit development. Food Chem. 326, 127034 (2020)
Amaral, M.S., Loures, C.C.A., Pedro, G.A., Reis, C.E.R., De Castro, H.F., Naves, F.L., Silva, M.B., Prata, A.M.R.: An unconventional two-stage cultivation strategy to increase the lipid content and enhance the fatty acid profile on Chlorella minutissima biomass cultivated in a novel internal light integrated photobioreactor aiming at biodiesel production. Renew. Energy. 156, 591–601 (2020)
Piasecka, A., Cieśla, J., Koczańska, M., Krzemińska, I.: Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods. J. Appl. Phycol. 31, 97–107 (2019)
Acknowledgements
Authors are also thankful to the management and team of JDW group of sugar mills Pakistan for provision of resources and technical support for this research.
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Maria Hasnain, Neelma Munir: Conceptualization, Investigation, Formal analysis, Methodology. Maria Hasnain, Faraz Ali: Writing – original draft. Supervision, Conceptualization and review & editing: Zamin Shaheed Siddiqui, Ali El-Keblawy and Zainul Abideen.
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Hasnain, M., Munir, N., Siddiqui, Z.S. et al. Integral Approach for the Evaluation of Sugar Cane Bio-Waste Molasses and Effects on Algal Lipids and Biodiesel Production. Waste Biomass Valor 14, 23–42 (2023). https://doi.org/10.1007/s12649-022-01864-0
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DOI: https://doi.org/10.1007/s12649-022-01864-0