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Alginate Lyase Produced by Filamentous Fungus Through Solid State Fermentation Using Sargassum from the Brazilian Coast

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Abstract

This article aimed at studying semi-solid-state fermentation using filamentous fungus for alginate lyase production using residual macroalgae as a substrate. Sargassum was the macroalgae chosen, given its wide abundance in the Brazilian coast. Among the fungal strains tested, Cunninghamella echinulata proved to be the most promising species. This work initially studied the optimisation of a nutrient solution (NS) in terms of nitrogen content, having verified that an increasing in peptone concentration (5 g/L) and the addition of yeast extract (2 g/L) (denominated NS2) led to a satisfactory fungal development, doubling alginate lyase production. Regarding moisture content, an increasing of enzyme production was verified when 65–75% of moisture content was used, with production peak reaching between 175 and 200 U/mL. The use of inoculum concentration between 2.106 and 1.107 spores/gbiomass did not have a significant influence on alginate lyase production. Moreover, the addition of an inducer (sodium alginate) increased enzyme production by 43%, shifting enzyme activity peak from 175 to 250 U/ml, with mass biomass substitution between 16.66 and 33.33% (walginate/wtotal). The results showed that higher NS2 pH (7–8.5) accelerated the production peak, which was higher than in the assays carried out with lower pH. Finally, stability of the enzyme extract to freezing was verified.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request through a material transfer agreement.

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Funding

C.E.F.S would like to thank the National the National Research Council and Technological Development (CNPq), Project Numbers: 167490/2017-6, 407274/2018-9 and 313195/2019-6.

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Authors and Affiliations

  1. Institute of Chemistry and Biotechnology, Federal University of Alagoas, Campus A. C. Simões, Maceió, AL, Brazil

    Margarete Cabral dos Santos Silva & Josealdo Tonholo

  2. Center of Technology, Federal University of Alagoas, Campus A. C. Simões, Maceió, AL, Brazil

    Carlos Eduardo De Farias Silva, Leandro Monteiro dos Santos, Josimayra Almeida Medeiros, Rosana Correia Vieira & Renata Maria Rosas Garcia Almeida

  3. Department of Food Technology, Federal University of Sergipe, São Cristovão, SE, Brazil

    Ana Karla de Souza Abud

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  1. Margarete Cabral dos Santos Silva
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dos Santos Silva, M.C., De Farias Silva, C.E., dos Santos, L.M. et al. Alginate Lyase Produced by Filamentous Fungus Through Solid State Fermentation Using Sargassum from the Brazilian Coast. Waste Biomass Valor 13, 2947–2962 (2022). https://doi.org/10.1007/s12649-022-01706-z

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