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No associations of 25-hydroxycholecalciferol and parathyroid hormone concentrations with calcaneal bone characteristics in community-dwelling elderly subjects: A cross-sectional study

  • Published:
The journal of nutrition, health & aging

Abstract

Objective

This study examined the associations of 25-hydroxycholecalciferol [25(OH)D3] and intact parathyroid hormone (iPTH) concentrations with bone status parameters in elderly subjects.

Design/Setting

Cross-sectional data based on the follow-up 2008 of the longitudinal study on nutrition and health status of senior citizens in Giessen, Germany.

Participants

One hundred eighty-eight independently living subjects aged 66–96 years.

Measurements

Serum concentrations of 25(OH)D3, iPTH, N-terminal propeptide of type 1 collagen (P1NP) and activity of alkaline phosphatase (ALP) were assessed. Broadband ultrasound attenuation, speed of sound and stiffness index were determined by calcaneal quantitative ultrasound (QUS). Multiple linear regression analyses were performed to analyse associations of 25(OH)D3 and iPTH with bone status parameters.

Results

Median (range) 25(OH)D3 and iPTH concentrations were 62.8 (29.9–106.7) nmol/L and 4.6 (1.3–21.0) pmol/L, respectively. Neither 25(OH)D3 nor iPTH was associated with calcaneal bone characteristics measured by QUS, whereas negative associations between 25(OH)D3 and bone formation markers (P1NP and ALP) were found. In a sub analysis, 25(OH)D3 was negatively associated with ALP only in subjects with iPTH concentrations > 4.59 pmol/L.

Conclusion

The present study provides no evidence for independent associations of 25(OH)D3 and iPTH with calcaneal bone characteristics in community-dwelling elderly subjects with 25(OH)D3 concentrations between 30 and 107 nmol/L. However, 25(OH)D3 interacts with bone formation markers, particularly in subjects with high iPTH concentrations.

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Abbreviations

25(OH)D:

25-hydroxyvitamin D

25(OH)D3 :

25-hydroxycholecalciferol

ALP:

alkaline phosphatase

B:

non-standardised coefficient beta

ß:

standardised coefficient beta

BMD:

bone mineral density

BMI:

body mass index

BUA:

broadband ultrasound attenuation

DXA:

dual-energy X-ray absorptiometry

ECLIA:

electrochemiluminescence immunoassay

eGFR:

estimated glomerular filtration rate

FSH:

follicle stimulating hormone

GISELA:

longitudinal study on nutrition and health status of senior citizens in Giessen

HRT:

hormone replacement therapy

iPTH:

intact parathyroid hormone

LH:

luteinising hormone

P1NP:

N-terminal propeptide of type 1 collagen

QUS:

quantitative ultrasound

R²:

adjusted coefficient of determination

SOS:

speed of sound

SI:

stiffness index

TSH:

thyroid stimulating hormone

References

  1. Arabi A, Baddoura R, Awada H, Salamoun M, Ayoub G, El-Hajj Fuleihan G. Hypovitaminosis D osteopathy: is it mediated through PTH, lean mass, or is it a direct effect? Bone 2006;39: 268–75.

  2. Arabi A, Baddoura R, El-Rassi R, El-Hajj Fuleihan G. PTH level but not 25 (OH) vitamin D level predicts bone loss rates in the elderly. Osteoporos Int 2012;23:971–80.

    Article  CAS  PubMed  Google Scholar 

  3. Bhattoa HP, Nagy E, More C, Kappelmayer J, Balogh A, Kalina E, Antal-Szalmas P. Prevalence and seasonal variation of hypovitaminosis D and its relationship to bone metabolism in healthy Hungarian men over 50 years of age: the HunMen Study. Osteoporos Int 2013;24: 179–86.

    Article  CAS  PubMed  Google Scholar 

  4. Garnero P, Munoz F, Sornay-Rendu E, Delmas PD. Associations of vitamin D status with bone mineral density, bone turnover, bone loss and fracture risk in healthy postmenopausal women. The OFELY study. Bone 2007;40: 716–22.

    Article  CAS  PubMed  Google Scholar 

  5. Ilich JZ, Brownbill RA, Tamborini L. Bone and nutrition in elderly women: protein, energy, and calcium as main determinants of bone mineral density. Eur J Clin Nutr 2003;57: 554–65.

    Article  CAS  PubMed  Google Scholar 

  6. Kauppi M, Impivaara O, Mäki J, Heliövaara M, Marniemi J, Montonen J, Jula A. Vitamin D status and common risk factors for bone fragility as determinants of quantitative ultrasound variables in a nationally representative population sample. Bone 2009;45: 119–24.

    Article  CAS  PubMed  Google Scholar 

  7. Kuchuk NO, Pluijm SM, van Schoor NM, Looman CW, Smit JH, Lips P. Relationships of serum 25-hydroxyvitamin D to bone mineral density and serum parathyroid hormone and markers of bone turnover in older persons. J Clin Endocrinol Metab 2009;94: 1244–50.

    Article  PubMed  Google Scholar 

  8. Macdonald HM, Mavroeidi A, Barr RJ, Black AJ, Fraser WD, Reid DM. Vitamin D status in postmenopausal women living at higher latitudes in the UK in relation to bone health, overweight, sunlight exposure and dietary vitamin D. Bone 2008;42: 996–1003.

    Article  CAS  PubMed  Google Scholar 

  9. Olmos JM, Hernández JL, García-Velasco P, Martínez J, Llorca J, González-Macías J. Serum 25-hydroxyvitamin D, parathyroid hormone, calcium intake, and bone mineral density in Spanish adults. Osteoporos Int 2016;27: 105–13.

    Article  CAS  PubMed  Google Scholar 

  10. Saquib N, von Mühlen D, Garland CF, Barrett-Connor E. Serum 25-hydroxyvitamin D, parathyroid hormone, and bone mineral density in men: the Rancho Bernardo study. Osteoporos Int 2006;17: 1734–41.

    Article  CAS  PubMed  Google Scholar 

  11. Sohl E, de Jongh RT, Swart KM, Enneman AW, van Wijngaarden JP, van Dijk SC, Ham AC, van der Zwaluw NL, Brouwer-Brolsma EM, van der Velde N, de Groot CP, te Velde SJ, Lips P, van Schoor NM. The association between vitamin D status and parameters for bone density and quality is modified by body mass index. Calcif Tissue Int 2015;96: 113–22.

    Article  CAS  PubMed  Google Scholar 

  12. van Ballegooijen AJ, Robinson-Cohen C, Katz R, Criqui M, Budoff M, Li D, Siscovick D, Hoofnagle A, Shea SJ, Burke G, de Boer IH, Kestenbaum B. Vitamin D metabolites and bone mineral density: the multi-ethnic study of atherosclerosis. Bone 2015;78: 186–93.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Vanderschueren D, Pye SR, O’Neill TW, Lee DM, Jans I, Billen J, Gielen E, Laurent M, Claessens F, Adams JE, Ward KA, Bartfai G, Casanueva FF, Finn JD, Forti G, Giwercman A, Han TS, Huhtaniemi IT, Kula K, Lean ME, Pendleton N, Punab M, Wu FC, Boonen S. Active vitamin D (1,25-dihydroxyvitamin D) and bone health in middle-aged and elderly men: the European Male Aging Study (EMAS). J Clin Endocrinol Metab 2013;98: 995–1005.

    Article  CAS  PubMed  Google Scholar 

  14. Zhen D, Liu L, Guan C, Zhao N, Tang X. High prevalence of vitamin D deficiency among middle-aged and elderly individuals in northwestern China: its relationship to osteoporosis and lifestyle factors. Bone 2015;71: 1–6.

    Article  CAS  PubMed  Google Scholar 

  15. Zochling J, Sitoh YY, Lau TC, Cameron ID, Cumming RG, Lord SR, Schwarz J, Trube A, March LM, Sambrook PN. Quantitative ultrasound of the calcaneus and falls risk in the institutionalized elderly: sex differences and relationship to vitamin D status. Osteoporos Int 2002;13: 882–7.

    Article  CAS  PubMed  Google Scholar 

  16. Avenell A, Mak JC, O’Connell D. Vitamin D and vitamin D analogues for preventing fractures in post-menopausal women and older men. Cochrane Database Syst Rev 2014;4:CD000227.

    Google Scholar 

  17. Rizzoli R, Boonen S, Brandi ML, Bruyère O, Cooper C, Kanis JA, Kaufman JM, Ringe JD, Weryha G, Reginster JY. Vitamin D supplementation in elderly or postmenopausal women: a 2013 update of the 2008 recommendations from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO). Curr Med Res Opin 2013;29: 305–13.

    Article  CAS  PubMed  Google Scholar 

  18. Ross AC, Manson JE, Abrams SA, Aloia JF, Brannon PM, Clinton SK, Durazo-Arvizu RA, Gallagher JC, Gallo RL, Jones G, Kovacs CS, Mayne ST, Rosen CJ, Shapses SA. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know. J Clin Endocrinol Metab 2011;96: 53–8.

    Article  CAS  PubMed  Google Scholar 

  19. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, Murad MH, Weaver CM. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 2011;96: 1911–30.

    Article  CAS  PubMed  Google Scholar 

  20. Kanis JA. Diagnosis of osteoporosis and assessment of fracture risk. Lancet 2002;359: 1929–36.

    Article  PubMed  Google Scholar 

  21. Blanchet C, Giguère Y, Prud’homme D, Turcot-Lemay L, Dumont M, Leduc G, Côte S, Laflamme N, Rousseau F, Dodin S. Leisure physical activity is associated with quantitative ultrasound measurements independently of bone mineral density in postmenopausal women. Calcif Tissue Int 2003;73: 339–49.

    Article  CAS  PubMed  Google Scholar 

  22. Martínez J, Olmos JM, Hernández JL, Pinedo G, Llorca J, Obregón E, Valero C, González-Macías J. Bone turnover markers in Spanish postmenopausal women: the Camargo cohort study. Clin Chim Acta 2009;409: 70–4.

    Article  PubMed  Google Scholar 

  23. Khosla S. Pathogenesis of age-related bone loss in humans. J Gerontol A Biol Sci Med Sci 2013;68: 1226–35.

    Article  CAS  PubMed  Google Scholar 

  24. Jungert A, Neuhäuser-Berthold M. Sex-specific determinants of serum 25-hydroxyvitamin D3 concentrations in an elderly German cohort: a cross-sectional study. Nutr Metab (Lond) 2015;12:2.

    Article  Google Scholar 

  25. Alcaraz A, González-López R, Morote J, de la Piedra C, Meseguer C, Esteban E, Climent M, González-Gragera B, Alvarez-Ossorio JL, Chirivella I, et al. Biochemical markers of bone turnover and clinical outcome in patients with renal cell and bladder carcinoma with bone metastases following treatment with zoledronic acid: The TUGAMO study. Br J Cancer 2013;109: 121–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Dusso A, González EA, Martin KJ. Vitamin D in chronic kidney disease. Best Pract Res Clin Endocrinol Metab 2011;25: 647–55.

    Article  CAS  PubMed  Google Scholar 

  27. Vasikaran SD, Chubb SP, Ebeling PR, Jenkins N, Jones GR, Kotowicz MA, Morris HA, Schneider HG, Seibel MJ, Ward G. Harmonised Australian reference intervals for serum PINP and CTX in adults. Clin Biochem Rev 2014;35: 237–42.

    PubMed  PubMed Central  Google Scholar 

  28. Woitge HW, Seibel MJ, Ziegler R. Comparison of total and bone-specific alkaline phosphatase in patients with nonskeletal disorder or metabolic bone diseases. Clin Chem 1996;42: 1796–804.

    CAS  PubMed  Google Scholar 

  29. Roche Diagnostics GmbH. Elecsys 1010/2010/Modular analytics E170. Parathyroid hormone PTH, intact. Mannheim, Germany: Roche Diagnostics; 2003.

  30. Roche Diagnostics GmbH. Elecsys 1010/2010 and Modular analytics E170, Cortisol. Mannheim, Germany: Roche Diagnostics; 2005.

  31. Roche Diagnostics GmbH. Elecsys and cobas analyzer. 25-Hydroxyvitamin D3, Vitamin D3 (25-OH). Mannheim, Germany: Roche Diagnostics; 2007.

  32. Roche Diagnostics GmbH. Elecsys and cobas e analyzers. Thyrotropin, TSH. Mannheim, Germany: Roche Diagnostics; 2007.

  33. Roche Diagnostics GmbH. Elecsys and cobas e analyzers. Total procollagen type 1 amino-terminal propeptide, total P1NP. Mannheim, Germany: Roche Diagnostics; 2007.

  34. Roche Diagnostics GmbH. Roche/Hitachi. Alkalische Phosphatase flüssig nach IFCC (alkaline phosphatase), ALP. Mannheim, Germany: Roche Diagnostics; 2008.

  35. Roche Diagnostics GmbH. Elecsys 1010/2010/Modular analytics E170. Follikelstimulierendes Hormon (follicle stimulating hormone), FSH. Mannheim, Germany: Roche Diagnostics; 2004.

  36. Roche Diagnostics GmbH. Elecsys 1010/2010/Modular analytics E170. Testosterone. Mannheim, Germany: Roche Diagnostics; 2004.

  37. Roche Diagnostics GmbH. Elecsys 1010/2010 and Modular analytics E170. Estradiol -E2. Mannheim, Germany: Roche Diagnostics; 2005.

  38. Roche Diagnostics GmbH. Elecsys 1010/2010 and Modular analytics E170. Luteinizing hormone, LH. Mannheim, Germany: Roche Diagnostics; 2005.

  39. Levey AS, Greene T, Kusek J, Beck G. A simplified equation to predict glomerular filtration rate from serum creatinine (abstract). J Am Soc Nephrol 2000;11:155A.

    Google Scholar 

  40. Edelmann-Schäfer B, Berthold LD, Stracke H, Lührmann PM, Neuhäuser-Berthold M. Identifying elderly women with osteoporosis by spinal dual X-ray absorptiometry, calcaneal quantitative ultrasound and spinal quantitative computed tomography: a comparative study. Ultrasound Med Biol 2011;37: 29–36.

    Article  PubMed  Google Scholar 

  41. Njeh CF, Blake GM. Calcaneal quantitative ultrasound devices: water coupled. In: Njeh CF, Hans D, Fuerst T, Glüer CC, Genant HK, (eds). Quantitative ultrasound. Assessment of osteoporosis and bone status. London: Martin Dunitz;1999. pp. 109–24.

    Google Scholar 

  42. Roubenoff R, Baumgartner RN, Harris TB, Dallal GE, Hannan MT, Economos CD, Stauber PM, Wilson PW, Kiel DP. Application of bioelectrical impedance analysis to elderly populations. J Gerontol A Biol Sci Med Sci 1997;52:M129–36.

    Article  CAS  PubMed  Google Scholar 

  43. Krems C, Lührmann PM, Neuhäuser-Berthold M. Physical activity in young and elderly subjects. J Sports Med Phys Fitness 2004;44: 71–6.

    CAS  PubMed  Google Scholar 

  44. Lührmann PM, Herbert BM, Gaster C, Neuhäuser-Berthold M. Validation of a self-administered 3-day estimated dietary record for use in the elderly. Eur J Nutr 1999;38: 235–40.

    Article  PubMed  Google Scholar 

  45. Michelsen J, Wallaschofski H, Friedrich N, Spielhagen C, Rettig R, Ittermann T, Nauck M, Hannemann A. Reference intervals for serum concentrations of three bone turnover markers for men and women. Bone 2013;57: 399–404.

    Article  CAS  PubMed  Google Scholar 

  46. Lee S, Teschemaker AR, Daniel M, Maneno MK, Johnson AA, Wutoh AK, Lee E. Calcium and vitamin D use among older adults in U.S.: results from national survey. J Nutr Health Aging 2016;20: 300–5.

    Article  CAS  PubMed  Google Scholar 

  47. Trimpou P, Bosaeus I, Bengtsson BA, Landin-Wilhelmsen K. High correlation between quantitative ultrasound and DXA during 7 years of follow-up. Eur J Radiol 2010;73: 360–4.

    Article  PubMed  Google Scholar 

  48. Olmos JM, Hernández JL, Martínez J, Pariente E, Llorca J, González-Macías J. Bone turnover markers in Spanish adult men The Camargo Cohort Study. Clin Chim Acta 2010;411: 1511–5.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Nutritional Science, Justus-Liebig-University, Goethestrasse 55, D-35390, Giessen, Germany

    A. Jungert & Monika Neuhäuser-Berthold

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Correspondence to Monika Neuhäuser-Berthold.

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Jungert, A., Neuhäuser-Berthold, M. No associations of 25-hydroxycholecalciferol and parathyroid hormone concentrations with calcaneal bone characteristics in community-dwelling elderly subjects: A cross-sectional study. J Nutr Health Aging 21, 733–742 (2017). https://doi.org/10.1007/s12603-016-0785-z

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  • DOI: https://doi.org/10.1007/s12603-016-0785-z

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