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Influence of high polyphenol beverage on stress-induced platelet activation

  • Published:
The journal of nutrition, health & aging

Abstract

Objectives

Platelets are playing a crucial role in acute cardiovascular events. We investigated if physical stress activates platelets and whether this activation can be inhibited by a polyphenol-enriched diet.

Methods

Blood samples were taken from a total of 103 athletes three weeks before, one day before, immediately as well as 24 hours and 72 hours after a marathon run. Participants were randomized, double-blinded and divided into two groups. One group received a polyphenol-rich beverage the other the same beverage without polyphenols. Besides analysis of platelet counts and impedance-aggregometric-measurement of platelet activity, soluble P-selectin and Endothelin-A measurements were performed.

Results

In the control group, runners showed a 2.2-fold increased platelet aggregation directly after completing a marathon and within the following three days when compared with baseline values (p<0.01). In accordance, significant increases in sP-selectin (57.52ng/ml vs. 94.86ng/ml;p<0.01) were detectable. In contrast, for the group consuming a beverage with increased polyphenol content (upper quartile of study beverage intake) we did not find any increase of platelet aggregation.

Discussion

Physical stress causes a significant increase in platelet activity. Our results demonstrate that a diet enriched in polyphenols is capable of preventing platelet activation. These findings might indicate a diminished cardiovascular stress-reaction following pre-exposition to polyphenol-enriched diet.

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Abbreviations

ADP:

adenosindiphosphat

AUC:

area under the curve

BeMaGIC:

Beer Marathon Genetics Inflammation and Cardiovascular System

BNP:

brain natriuretic peptide

cAMP:

cyclisches Adenosinmonophosphat

CGA:

Chromogranin A

ELISA:

enzyme linked immunosorbent assay

GbIIb/IIIa:

glycol-protein IIb-IIIa complex

GAE:

gallic acid equivalents

IQR:

interquartile range

NADPH:

nicotinamide adenine dinucleotide phosphate-oxidase

NO:

nitric oxide

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Department of Internal Medicine/Cardiology, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany

    Thomas Nickel, K. Lackermair, G. Waidhauser, H. Methe & U. Wilbert-Lampen

  2. Department of Prevention and Sports Medicine, Klinikum rechts der Isar, Technical University, Munich, Germany

    J. Scherr & M. Halle

  3. Department of Transfusion Medicine and Haemostaseology, Ludwig-Maximilians-University, Munich, Germany

    A. Calatzis

  4. Division of Sports Medicine, Institute of Exercise and Health Sciences, Medical Faculty, University of Basel, Basel, Switzerland

    H. Hanssen

  5. Department of Internal Medicine/Nephrology, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany

    U. Schönermark

  6. Department of Internal Medicine/Gastroenterology, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany

    S. Horster

  7. Institut for laboratory Medicine, University Hospital Grosshadern, Ludwig-Maximilians-University, Munich, Germany

    M. Vogeser

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  1. Thomas Nickel
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Correspondence to Thomas Nickel.

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These authors contributed equally to this work

Both senior authors contributed equally to this work.

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Nickel, T., Lackermair, K., Scherr, J. et al. Influence of high polyphenol beverage on stress-induced platelet activation. J Nutr Health Aging 20, 586–593 (2016). https://doi.org/10.1007/s12603-016-0697-y

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  • DOI: https://doi.org/10.1007/s12603-016-0697-y

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