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A Review on cLF36, a Novel Recombinant Antimicrobial Peptide-Derived Camel Lactoferrin

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Abstract

Lactoferrin is an antimicrobial peptide (AMP) playing a pivotal role in numerous biological processes. The primary antimicrobial efficacy of lactoferrin is associated with its N-terminal end, which contains various peptides, such as lactoferricin and lactoferrampin. In this context, our research team has developed a refined chimeric 42-mer peptide known as cLF36 over the past few years. This peptide encompasses the complete amino acid sequence of camel lactoferrampin and partial amino acid sequence of lactoferricin. The peptide’s activity against human, avian, and plant bacterial pathogens has been assessed using different biological platforms, including prokaryotic (P170 and pET) and eukaryotic (HEK293) expression systems. The peptide positively influenced the growth performance and intestinal morphology of chickens challenged with pathogen bacteria. Computational methods and in vitro studies showed the peptide’s antiviral effects against hepatitis C virus, influenza virus, and rotavirus. The chimeric peptide exhibited higher activity against certain tumor cell lines compared to normal cells, which may be attributed to the peptide’s interaction with negatively charged glycosaminoglycans on the surface of tumor cells. Importantly, this peptide exhibited no toxicity against host cells and demonstrated remarkable thermal and protease stability in serum. In conclusion, while our investigations suggest that the chimeric peptide, cLF36, may offer potential as a candidate or complementary option to some available antibiotics, antiviral agents, and chemical pesticides, significant uncertainties remain regarding its cost-effectiveness, as well as its pharmacodynamic and pharmacokinetic characteristics, which require further elucidation.

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Authors and Affiliations

  1. Department of Pathobiology, Division of Biotechnology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Solmaz Morovati

  2. Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Amir Asghari Baghkheirati & Jamshid Razmyar

  3. Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Mohammad Hadi Sekhavati

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  3. Mohammad Hadi Sekhavati
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M.H.S., A.A.B., and J.R. had the idea for the article. S.M. and A.A.B. performed the literature search and data analysis. S.M. drafted the work. M.H.S. and J.R. revised the manuscript. All authors reviewed and approved the final manuscript.

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Morovati, S., Baghkheirati, A.A., Sekhavati, M.H. et al. A Review on cLF36, a Novel Recombinant Antimicrobial Peptide-Derived Camel Lactoferrin. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10285-5

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