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Comparative study of antimicrobial activity between some medicine plants and recombinant Lactoferrin peptide against some pathogens of cultivated button mushroom

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Abstract

The adverse effects of chemical pesticides on human health and environment cannot be ignored, hence it seems that novel alternative compounds should be applied to control plant pathogens. Among various alternative sources, natural compounds such as plant essential oils, plant extracts and recombinant antimicrobial peptides are of significance. The aim of the present study was to investigate antimicrobial activity of plants essential oils and plant extracts of six medicinal plants (Lippia citriodora, Ferula gummosa, Bunium persicum, Mentha piperita, Plantago major and Salvadora persica) along with a chimera peptide of camel lactoferrin, which is the most important antimicrobial component of camel milk, against Pseudomonas tolaasii and Trichoderma harzianum as pathogens of white button mushroom. The antibacterial activity test was conducted under in vitro conditions through disc diffusion method. The results showed that chimera camel lactoferrin peptide, with the highest amount of inhibitory zone (14.63 mm in 20 μg/mL concentration), has a significant difference in antibacterial activity compared to other treatments. Ferula gummosa conferred no antibacterial activity. Also, the results of antifungal effects indicated that plant essential oils and extracts have more antifungal activity than recombinant peptide. Generally, L. citriodora, B. persicum, M. piperita treatments could completely prevent growth of fungal in in vitro conditions. Therefore, using the mentioned plants can be a good replacement for reducing the chemical pesticides against pathogenic agents of button mushroom, without any adverse effects on environment and human health.

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Availability of data and material

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

EOs:

Essential oils

PEs:

Plant extracts

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Acknowledgements

We should thank the Animal Biotechnology Laboratory of Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran, And Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi university of Mashhad, Mashhad, Iran, to contribute to the success of this project

Funding

This study was financially supported by Ferdowsi university of Mashhad (Grant number 2/40709).

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Author notes

  1. Abbas Tanhaeian and Narges Nazifi contributed equally as first author.

Authors and Affiliations

  1. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Abbas Tanhaeian & Farajollah Shahriari Ahmadi

  2. Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Narges Nazifi

  3. Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Mahdi Akhlaghi

Authors
  1. Abbas Tanhaeian
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  2. Narges Nazifi
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  3. Farajollah Shahriari Ahmadi
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  4. Mahdi Akhlaghi
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Contributions

In brief, AB and FSH introduced the conception of this article. AB and MA contributed in preparation of the data. NN has been done the statistical analysis of this article. NN and FSH contributed in preparation of the manuscript. Authors read and approved the final manuscript.

Corresponding author

Correspondence to Farajollah Shahriari Ahmadi.

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The authors declare there is no conflict of interest.

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This study confirms that all experimental protocols were approved by Faculty of Agriculture, Ferdowsi university of Mashhad, Mashhad, Iran.

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Communicated by Erko Stackebrandt.

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Tanhaeian, A., Nazifi, N., Shahriari Ahmadi, F. et al. Comparative study of antimicrobial activity between some medicine plants and recombinant Lactoferrin peptide against some pathogens of cultivated button mushroom. Arch Microbiol 202, 2525–2532 (2020). https://doi.org/10.1007/s00203-020-01964-2

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  • DOI: https://doi.org/10.1007/s00203-020-01964-2

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