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Probiotic Properties of Lactobacillus Strains Isolated from Table Olive Biofilms

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Abstract

In this work, 16 strains with promising probiotic characteristics belonging to the Lactobacillus pentosus (13) and Lactobacillus plantarum (3) species and isolated from table olive biofilms were tested for adherence to cell lines and to solvents, immunomodulatory, and anti-proliferative properties on epithelial human cellular lines. Most Lactobacillus strains were able to regulate the production of cytokines by stimulating the production of pro-inflammatory (IL-6) and anti-inflammatory (IL-10) interleukins on macrophages, and by suppressing the secretion of IL-8 on HT-29 TNF-α-induced model. Lactobacillus strains also showed anti-proliferative activity on the HT-29 cell line. No clear relation was found between adhesion to solvents and adhesion to HT-29 human cell line. Lactobacillus pentosus LPG1, which showed the best anti-inflammatory and immunomodulatory properties, was then tested in a dinitro-benzene sulfonic acid (DNBS)-induced chronic colitis murine model. As a measure of the inflammation, gut permeability and weight loss, as well as cytokine profiles, were determined. Lactobacillus pentosus LPG1 improved mice health as observed by a significant reduction of weight loss, gut permeability, and beneficial cytokine modulation. Macroscopic scores and tissue damage were also lower in mice administered with LPG1 with respect to the DNBS-treated group. These results showed that L. pentosus LPG1 isolated from plant could have potential as probiotic for use as an anti-inflammatory and immunomodulatory agent for patients with inflammatory bowel disease.

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Acknowledgments

Antonio Benítez Cabello wishes to express his gratitude to the Commensal and Probiotics-Host Interactions team, from Micalis Institute (INRA), for their unconditional help in the development of this work.

Funding

The research has received funding from the Spanish Government (Project OliFilm AGL-2013-48300-R: www.olifilm.science.com.es). Antonio Benítez Cabello received predoctoral grant from the Spanish Ministry of Economy and Competitiveness.

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  1. Antonio Benítez-Cabello and Edgar Torres-Maravilla contributed equally to this work.

Authors and Affiliations

  1. Food Biotechnology Department, Instituto de la Grasa (CSIC), Ctra. Utrera km 1, Building 46, 41013, Seville, Spain

    Antonio Benítez-Cabello, Rufino Jiménez-Díaz & Francisco Noé Arroyo-López

  2. INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, 78350, Jouy-en-Josas, France

    Antonio Benítez-Cabello, Edgar Torres-Maravilla, Luis Bermúdez-Humarán, Philippe Langella & Rebeca Martín

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Benítez-Cabello, A., Torres-Maravilla, E., Bermúdez-Humarán, L. et al. Probiotic Properties of Lactobacillus Strains Isolated from Table Olive Biofilms. Probiotics & Antimicro. Prot. 12, 1071–1082 (2020). https://doi.org/10.1007/s12602-019-09604-y

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