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Identification of novel anti-inflammatory probiotic strains isolated from pulque

  • Applied Microbial and Cell Physiology
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Abstract

Probiotics are live microorganisms which when administered in adequate amounts, confer health benefits on the host. Their use is more and more widespread for both prevention and treatment of diseases, including traveler’s diarrhea and inflammatory bowel diseases (IBDs). In this work, we isolated and characterized novel candidate probiotic strains from pulque (xaxtle), a traditional Mexican alcoholic fermented beverage. A total of 14 strains were obtained from xaxtle samples isolated from three different Mexican regions. Species identification was performed by biochemical methods and 16S rRNA gene targeted PCR. The isolates belonged to the Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus brevis, and Lactobacillus composti phylogenetic groups, with L. brevis being the most dominant group. Bacteria were tested for lysozyme, low pH, and bile acid resistance. Moreover, the strains were tested for adherence to human intestinal epithelial cells and screened for their immunomodulatory properties using a cellular model. Selected bacterial strains with anti-inflammatory properties were then tested in vivo in a dinitro-benzene sulfonic acid (DNBS)-induced chronic colitis mouse model, and weight loss, gut permeability, and cytokine profiles were measured as readouts of inflammation. One of the selected strains, Lactobacillus sanfranciscensis LBH1068, improved mice health as observed by a reduction of weight loss, significant decreases in gut permeability, and cytokine modulation. Altogether, our results highlighted the potential of lactobacilli isolated from pulque and in particular the strain L. sanfranciscensis LBH1068 as a novel probiotic to treat IBD.

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Acknowledgments

ETM received scholarships and doctoral fellowship mobility from the Consejo Nacional de Ciencia y Tecnología (CONACyT), México, and Coordinación de Cooperación Académica (CCA) from Institute Politécnico Nacional. ETM thanks P. Langella and Luis Bermúdez for their help and for the doctoral stay in the Microbiologie de l’Alimentation au service de la Santé (Micalis) Insitute, INRA-Jouy en Josas, France.

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Authors and Affiliations

  1. Escuela Nacional de Ciencias Biológicas, IPN, Departamento de Ingeniería Bioquímica, Laboratorio de Investigación en Alimentos, Distrito Federal, México

    Edgar Torres-Maravilla & María E. Sánchez-Pardo

  2. INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, F-78350, Jouy-en-Josas, France

    Edgar Torres-Maravilla, Marion Lenoir, Thibault Allain, Harry Sokol, Philippe Langella & Luis G. Bermúdez-Humarán

  3. AgroParisTech, UMR1319 Micalis, F-78350, Jouy-en-Josas, France

    Edgar Torres-Maravilla, Marion Lenoir, Thibault Allain, Harry Sokol, Philippe Langella & Luis G. Bermúdez-Humarán

  4. Universidad Autónoma Metropolitana, Unidad Xochimilco, Departamento de Sistemas Biológicos, Laboratorio de Biotecnología, Distrito Federal, México

    Lino Mayorga-Reyes

  5. INSERM, Equipe AVENIR U1057 / UMR CNRS 7203, 75012, Paris, France

    Harry Sokol

  6. Department of Gastroenterology and Nutrition, AP-HP, Hôpital Saint-Antoine F-75012 and UPMC Univ Paris 06F-75005, Paris, France

    Harry Sokol

Authors
  1. Edgar Torres-Maravilla
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  2. Marion Lenoir
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  3. Lino Mayorga-Reyes
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  4. Thibault Allain
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  5. Harry Sokol
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  6. Philippe Langella
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  7. María E. Sánchez-Pardo
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  8. Luis G. Bermúdez-Humarán
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Corresponding authors

Correspondence to María E. Sánchez-Pardo or Luis G. Bermúdez-Humarán.

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Funding

This work was partially supported by the project IPN-SIP-20130135 from the Secretaría de Investigación y Posgrado, Mexico.

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All authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Torres-Maravilla, E., Lenoir, M., Mayorga-Reyes, L. et al. Identification of novel anti-inflammatory probiotic strains isolated from pulque. Appl Microbiol Biotechnol 100, 385–396 (2016). https://doi.org/10.1007/s00253-015-7049-4

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