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Effects of Dietary Clostridium butyricum on the Growth, Digestive Enzyme Activity, Antioxidant Capacity, and Resistance to Nitrite Stress of Penaeus monodon

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Abstract

The present study investigated the effects of the dietary probiotic Clostridium butyricum (CB) on the growth, intestine digestive enzyme activity, antioxidant capacity and resistance to nitrite stress, and body composition of Penaeus monodon. For 56 days, shrimps were fed diets containing different levels of C. butyricum (1 × 109 CFU g−1), 0% (control), 0.5% (CB1), 1.0% (CB2), and 2.0% (CB3), as treatment groups, followed by an acute nitrite stress test for 48 h. The results indicated that dietary supplementation of C. butyricum increased the growth of shrimp in the CB2 and CB3 groups. The survival rate of shrimp increased after nitrite stress for 24 and 48 h. The intestine amylase and trypsin activities increased in all three C. butyricum groups, while the lipase activity was only affected in the CB3 group. The superoxide dismutase (SOD) activity as well as heat shock protein 70 (hsp70) and ferritin gene expression levels were increased in the intestines of shrimps cultured under normal conditions for 56 days, while the catalase (CAT) activity was not changed and glutathione peroxidase (GPx) activity was only increased in the CB2 and CB3 groups. After exposure to nitrite stress for 24 and 48 h, the intestine antioxidant enzyme (SOD, CAT, and GPx) activity and gene (hsp70 and ferritin) expression levels in the three C. butyricum groups were higher than those of the control. C. butyricum had no effects on the whole body composition of the shrimp. These results revealed that C. butyricum improved the growth as well as enhanced the intestine digestive enzyme and antioxidant activities of P. monodon against nitrite stress, and C. butyricum may be a good probiotic for shrimp aquaculture.

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Acknowledgments

The authors were grateful to all the laboratory members for experimental material preparation and technical assistance. This study was supported by the earmarked fund for Guangdong Provincial Special Fund for Marine Fisheries Technology (A201701B09), Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, People’s Republic of China (FREU2017-01), Guangdong Natural Science Foundation (2017A030313147), Guangdong Provincial Key Laboratory of Fishery Ecology and Environment (LFE-2016-12), Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS (2016TS07), Shenzhen Science and Technology Planning Project (JCYJ20170412110605075).

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Authors and Affiliations

  1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People’s Republic of China

    Yafei Duan, Jiasong Zhang, Jianhua Huang & Shigui Jiang

  2. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, People’s Republic of China

    Jianhua Huang

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  1. Yafei Duan
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Correspondence to Jiasong Zhang or Jianhua Huang.

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The authors declare no competing financial interests.

Ethical Approval

The collection and handling of the animals in this study was approved by the Animal Care and Use Committee at the Chinese Academy of Fishery Sciences, and all experimental animal protocols were carried out in accordance with national and institutional guidelines for the care and use of laboratory animals at the Chinese Academy of Fishery Sciences.

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Highlights

• Effect of dietary Clostridium butyricum supplementation in Penaeus monodon was studied.

• C. butyricum improved the growth performance and feed utilization of P. monodon.

• C. butyricum influenced the intestine digestive enzyme of P. monodon.

• C. butyricum increased the intestine antioxidant capacity of P. monodon resistance to nitrite stress.

• C. butyricum had no effect on the body composition of P. monodon.

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Duan, Y., Zhang, J., Huang, J. et al. Effects of Dietary Clostridium butyricum on the Growth, Digestive Enzyme Activity, Antioxidant Capacity, and Resistance to Nitrite Stress of Penaeus monodon. Probiotics & Antimicro. Prot. 11, 938–945 (2019). https://doi.org/10.1007/s12602-018-9421-z

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