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Interspecific Interactions Among Members of Family Halobacteriaceae from Natural Solar Salterns

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Abstract

Solar salterns are crystallizer ponds with highly diverse extremophilic microbial flora living individually or in consortium. Twenty-four culturable haloarchaeal isolates were obtained from solar salterns of Goa, which were grouped under Halococcus, Haloferax, Haloarcula and Halorubrum. Cell-free supernatants of different isolates were checked against each other by pour plate technique combined with agar well diffusion method. This resulted in a zone of growth inhibition or stimulation around wells, indicating that some isolates had antagonistic and/or a beneficial effect on the other genera. Thus, members of family Halobacteriaceae were found to secrete extracellular metabolites, which can act as growth enhancers or repressors.

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Abbreviations

CFS:

Cell-free supernatant

Har :

Haloarcula

Hfx :

Haloferax

Hcc :

Halococcus

Hrr :

Halorubrum

AWDM:

Agar well diffusion method

BLAST:

Basic Local Alignment Search Tool

PAGE:

Polyacrylamide gel electrophoresis

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Acknowledgments

This work was supported by University Grants Commission, India (UGC) Major Research Project No: 34-500/2008. The authors thank Dr Hiroaki Minegishi, Bio-Nano Electronics Research Center, Toyo University, Japan for identifying the haloarchaeal isolates.

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Authors and Affiliations

  1. Department of Biological Sciences, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Zuarinagar, 403726, Goa, India

    Bhakti B. Salgaonkar, Kabilan Mani, Anjana Nair, Sowmya Gangadharan & Judith M. Braganca

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  1. Bhakti B. Salgaonkar
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  2. Kabilan Mani
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  3. Anjana Nair
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  4. Sowmya Gangadharan
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  5. Judith M. Braganca
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Correspondence to Judith M. Braganca.

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Salgaonkar, B.B., Mani, K., Nair, A. et al. Interspecific Interactions Among Members of Family Halobacteriaceae from Natural Solar Salterns. Probiotics & Antimicro. Prot. 4, 98–107 (2012). https://doi.org/10.1007/s12602-012-9097-8

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