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Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a

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Abstract.

The production of halocins, bacteriocin-like proteins of ecological significance, is a frequent characteristic of species from the family Halobacteriaceae. Halocin H1, produced by Haloferax mediterranei strain M2a, is a single 31-kDa polypeptide. Its purification was achieved by combining two chromatographic systems: Sepharose 4B linked to bacitracin followed by hydroxylapatite Bio-gel HTP. Halocin H1 required concentrations of NaCl higher than 1.5 M to maintain its activity. Haoarchaeal strains showed a differential degree of sensitivity to the action of this halocin.

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Authors and Affiliations

  1. Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain, Spain

    Gonzalo Platas & Ricardo Amils

  2. División de Microbiología, Campus de Elche, Universidad Miguel Hernández, 03202 Elche, Alicante, Spain, Spain

    Inmaculada Meseguer

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  1. Gonzalo Platas
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  2. Inmaculada Meseguer
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  3. Ricardo Amils
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Platas, G., Meseguer, I. & Amils, R. Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a. Int Microbiol 5, 15–19 (2002). https://doi.org/10.1007/s10123-002-0053-4

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  • DOI: https://doi.org/10.1007/s10123-002-0053-4

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