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The potential roles of bacterial communities in coral defence: A case study at Talang-talang reef

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Abstract

Complex microbial communities are known to exert significant influence over coral reef ecosystems. The Talang- Satang National Park is situated off the coast of Sematan and is one of the most diverse ecosystems found off-Sarawak. Interestingly, the Talang-talang reef thrives at above-average temperatures of 28- 30°C throughout the year. Through isolation and identification (16S rRNA) of native microbes from the coral, the surface mucus layer (SML), as well as the surrounding sediment and waters, we were able to determine the species composition and abundance of the culturable bacteria in the coral reef ecosystem. Isolates found attached to the coral are related mostly to Vibrio spp., presumably attached to the mucus from the water column and surrounding sediment. Pathogenic Vibrio spp. and Bacillus spp. were dominant amongst the isolates from the water column and sediment, while known coral pathogens responsible for coral bleaching, Vibrio coralliilyticus and Vibrio shiloi, were isolated from the coral SML and sediment samples respectively. Coral SML isolates were found to be closely related to known nitrogen fixers and antibiotic producers with tolerance towards elevated temperatures and heavy metal contamination, offering a possible explanation why the local corals are able to thrive in higher than usual temperatures. This specialized microbiota may be important for protecting the corals from pathogens by occupying entry niches and/or through the production of secondary metabolites such as antibiotics. The communities from the coral SML were tested against each other at 28, 30 and 32°C, and were also assessed for the presence of type I modular polyketides synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes which are both involved in the production of antibiotic compounds. The bacterial community from the SML exhibited antimicrobial properties under normal temperatures while pathogenic strains appeared toxic at elevated temperatures and our results highlight the role of the coral SML bacterial community in the coral’s defence.

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  1. Faculty of Engineering, Computing and Science, Swinburne University of Technology, Sarawak campus, 93350, Kuching, Sarawak, Malaysia

    Felicity W. I. Kuek, Li-Fang Lim, Lin-Hui Ngu & Moritz Müller

  2. Department of Aquatic Science, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 93400, Kota Samarahan, Sarawak, Malaysia

    Aazani Mujahid

  3. Bachok Marine Research Station, Institute of Ocean and Earth Science, University of Malaya, 16310, Bachok, Kelantan, Malaysia

    Po-Teen Lim & Chui-Pin Leaw

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  1. Felicity W. I. Kuek
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  2. Li-Fang Lim
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Kuek, F.W.I., Lim, LF., Ngu, LH. et al. The potential roles of bacterial communities in coral defence: A case study at Talang-talang reef. Ocean Sci. J. 50, 269–282 (2015). https://doi.org/10.1007/s12601-015-0024-2

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