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Diversity of Antibiotic-Active Bacteria Associated with the Brown Alga Laminaria saccharina from the Baltic Sea

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Abstract

Bacteria associated with the marine macroalga Laminaria saccharina, collected from the Kiel Fjord (Baltic Sea, Germany), were isolated and tested for antimicrobial activity. From a total of 210 isolates, 103 strains inhibited the growth of at least one microorganism from the test panel including Gram-negative and Gram-positive bacteria as well as a yeast. Most common profiles were the inhibition of Bacillus subtilis only (30%), B. subtilis and Staphylococcus lentus (25%), and B. subtilis, S. lentus, and Candida albicans (11%). In summary, the antibiotic-active isolates covered 15 different activity patterns suggesting various modes of action. On the basis of 16S rRNA gene sequence similarities >99%, 45 phylotypes were defined, which were classified into 21 genera belonging to Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Phylogenetic analysis of 16S rRNA gene sequences revealed that four isolates possibly represent novel species or even genera. In conclusion, L. saccharina represents a promising source for the isolation of new bacterial taxa and antimicrobially active bacteria.

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Acknowledgements

This study was supported by the Ministerium für Wissenschaft, Wirtschaft und Verkehr des Landes Schleswig-Holstein, Germany (project “Isolierung und Charakterisierung neuer Wirkstoffe aus Laminaria saccharina, Halichondria panicea und assoziierten Mikroorganismen” and the Kieler Wirkstoff-Zentrum am IFM-GEOMAR). We thank CRM and MariLim, both located at Kiel (Germany), for taking the L. saccharina samples. Special thanks go to Katja Kulke and Regine Wicher for technical assistance.

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  1. Leibniz-Institut für Meereswissenschaften IFM-GEOMAR, Düsternbrooker Weg 20, 24105, Kiel, Germany

    Jutta Wiese, Vera Thiel, Kerstin Nagel, Tim Staufenberger & Johannes F. Imhoff

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  1. Jutta Wiese
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  2. Vera Thiel
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Correspondence to Johannes F. Imhoff.

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Wiese, J., Thiel, V., Nagel, K. et al. Diversity of Antibiotic-Active Bacteria Associated with the Brown Alga Laminaria saccharina from the Baltic Sea. Mar Biotechnol 11, 287–300 (2009). https://doi.org/10.1007/s10126-008-9143-4

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