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Gene electrotransfer: from biophysical mechanisms to in vivo applications

Part 2 - In vivo developments and present clinical applications

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Abstract

Gene electrotransfer can be obtained not just on single cells in diluted suspension. For more than 10 years, this is a quasi routine strategy in tissue on the living animal and a few clinical trials have now been approved. New problems have been brought by the close contacts of cells in tissue both on the local field distribution and on the access of DNA to target cells. They need to be solved to provide a further improvement in the efficacy and safety of protein expression. There is a competition between gene transfer and cell destruction. Nevertheless, present results are indicative that electrotransfer is a promising approach for gene therapy. High level and long-lived expression of proteins can be obtained in muscles. This is used for a successful method of electrovaccination.

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Acknowledgements

This work was supported by the CNRS, the AFM (Association Française pour les Myopathies) and the region Midi-Pyrénées.

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  1. CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse UPS, 205 route de Narbonne, 31077, Toulouse, France

    Jean-Michel Escoffre, Chloé Mauroy, Thomas Portet, Luc Wasungu, Aurelie Paganin-Gioanni, Muriel Golzio, Justin Teissié & Marie-Pierre Rols

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  1. Jean-Michel Escoffre
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  2. Chloé Mauroy
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Correspondence to Justin Teissié or Marie-Pierre Rols.

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Jean-Michel Escoffre and Chloé Mauroy have contributed equally to this work.

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Escoffre, JM., Mauroy, C., Portet, T. et al. Gene electrotransfer: from biophysical mechanisms to in vivo applications. Biophys Rev 1, 185–191 (2009). https://doi.org/10.1007/s12551-009-0019-2

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