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Analysis of the genetic variability of Artemia franciscana Kellogg, 1906 from the Great Salt Lake (USA) based on mtDNA sequences, ISSR genomic fingerprinting and biometry

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Abstract

The genetic structure of the brine shrimp Artemia franciscana, an extremophile halophilic microcrustacean, from the Great Salt Lake (USA), was investigated by analysis of nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI), genomic fingerprinting by ISSR-PCR (inter-simple sequence repeats polymerase chain reaction) and biometry. Samples from six different geographical localities of Great Salt Lake revealed ten distinct haplotypes with a mean high level of haplotype diversity (HD = 0.6). Phylogenetic and genetic fingerprinting analyses supported the null hypothesis, that the brine shrimps of this lake form a panmictic gene pool with a low level of genetic differentiation index (F ST  = 0.05) and a high rate of gene flow (Nm = 2.8) between geographical areas. Moreover, morphological parameters revealed no population structure among all examined populations. Neutrality tests and mismatch distribution revealed that A. franciscana has undergone a recent population expansion. In spite of hypersaline conditions in the Northern (Gunnison Bay) regions of the Great Salt Lake, the genetic population structures of different areas of the lake are not linked to ecological or limnological conditions. Therefore, A. franciscana from the Great Salt Lake should be considered as a single management unit for conservation.

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Acknowledgments

Amin Eimanifar is grateful to the Deutscher Akademischer Austauschdienst (DAAD) for a Ph.D. fellowship. We would like to express our appreciation to Dr. Alex Petrov, who provided samples of A. franciscana from the GSL, Utah.

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  1. Amin Eimanifar

    Present address: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany

Authors and Affiliations

  1. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany

    Markus Santhosh Braun & Michael Wink

  2. Parliament Fisheries, Inc., 2432 Taylor Avenue, Ogden, UT, 84401, USA

    Brad Marden

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  1. Amin Eimanifar
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Eimanifar, A., Marden, B., Braun, M.S. et al. Analysis of the genetic variability of Artemia franciscana Kellogg, 1906 from the Great Salt Lake (USA) based on mtDNA sequences, ISSR genomic fingerprinting and biometry. Mar Biodiv 45, 311–319 (2015). https://doi.org/10.1007/s12526-014-0256-x

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  • DOI: https://doi.org/10.1007/s12526-014-0256-x

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