Skip to main content
Log in

Effect of Sugarcane Jaggery Prepared Using Different Heat Treatment Methods on α-Amylase Activity

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

The focus of the present investigation was to provide a comparative note on the α-amylase inhibition efficiency among jaggery samples prepared at high temperature (open-pan method) and low thermal conditions (vacuum evaporation). A lyophilized sugarcane juice sample was taken as a control. Water, methanol, and ethyl acetate were used as extraction solvents to extract biomolecules from produced jaggery. At a concentration of 16.5 mg mL−1, open-pan jaggery inhibited α-amylase by 33.14%. The inhibitory activity indicated a good correlation with respect to total phenol content. The results highlight the rationale for further clinical studies to support jaggery as a better choice to manage type-2 diabetes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ahmed, M.S.H., H. Nayaka, Y.Q. Almulaiky, and S. Al-Dalali. 2021a. Evaluation of phytochemical screening and biological activity of lyophilized sugarcane juice, vacuum and open pan jaggery for aqueous extracts. Journal of Advanced Scientific Research 12 (01): 81–91.

    Article  CAS  Google Scholar 

  • Ahmed, N.H., Y.Q. Almulaiky, and S. Al-Dalali. 2021b. Comparative phytochemical screening and biological activity of “lyophilized sugarcane juice, vacuum pan and open pan jaggery” for methanolic extract. Research Journal of Agricultural Sciences 12: 391–398.

    Google Scholar 

  • Al-Asri, J., E. Fazekas, G. Lehoczki, A. Perdih, C. Görick, M.F. Melzig, and J. Mortier. 2015. From carbohydrates to drug-like fragments: Rational development of novel α-amylase inhibitors. Bioorganic & Medicinal Chemistry 23 (20): 6725–6732.

    Article  CAS  Google Scholar 

  • Aleixandre, A., J.V. Gil, J. Sineiro, and C.M. Rosell. 2022. Understanding phenolic acids inhibition of α-amylase and α-glucosidase and influence of reaction conditions. Food Chemistry 372: 131231.

    Article  CAS  PubMed  Google Scholar 

  • Ani, V., and K.A. Naidu. 2008. Antihyperglycemic activity of polyphenolic components of black/bitter cumin Centratherum anthelminticum (L.) Kuntze seeds. European Food Research and Technology 226 (4): 897–903.

    Article  CAS  Google Scholar 

  • Arruzazabala, M.L., D. Carbajal, R. Mas, V. Molina, S. Valdes, and A. Laguna. 1994. Cholesterol lowering effects of policosanol in rabbits. Biological Research 27: 205–208.

    CAS  PubMed  Google Scholar 

  • Asikin, Y., N. Hirose, H. Tamaki, S. Ito, H. Oku, and K. Wada. 2016. Effects of different drying–solidification processes on physical properties, volatile fraction, and antioxidant activity of non-centrifugal cane brown sugar. LWT-Food Science and Technology 66: 340–347.

    Article  CAS  Google Scholar 

  • Barrera, C., N. Betoret, and L. Seguí. 2020. Phenolic profile of cane sugar derivatives exhibiting antioxidant and antibacterial properties. Sugar Tech 22 (5): 798–811.

    Article  CAS  Google Scholar 

  • Barrett, M.L., and J.K. Udani. 2011. A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): A review of clinical studies on weight loss and glycemic control. Nutrition Journal 10: 24.

    Article  PubMed  PubMed Central  Google Scholar 

  • Barrett, A., T. Ndou, C.A. Hughey, C. Straut, A. Howell, Z. Dai, and G. Kaletunc. 2013. Inhibition of α-amylase and glucoamylase by tannins extracted from cocoa, pomegranates, cranberries, and grapes. Journal of Agricultural and Food Chemistry 61 (7): 1477–1486.

    Article  CAS  PubMed  Google Scholar 

  • Behl, T., S. Bungau, K. Kumar, G. Zengin, F. Khan, A. Kumar, and D.E. Mosteanu. 2020. Pleotropic effects of polyphenols in cardiovascular system. Biomedicine & Pharmacotherapy 130: 110714.

    Article  CAS  Google Scholar 

  • Betoret, E., and C.M. Rosell. 2020. Effect of particle size on functional properties of Brassica napobrassica leaves powder. Starch interactions and processing impact. Food Chemistry: X 8: 100106.

    CAS  PubMed  Google Scholar 

  • da Silva, S.M., E.A. Koehnlein, A. Bracht, R. Castoldi, G.R. de Morais, M.L. Baesso, and R.M. Peralta. 2014. Inhibition of salivary and pancreatic α-amylases by a pinhão coat (Araucaria angustifolia) extract rich in condensed tannin. Food Research International 56: 1–8.

    Article  Google Scholar 

  • Damager, I., S. Numao, H. Chen, G.D. Brayer, and S.G. Withers. 2004. Synthesis and characterisation of novel chromogenic substrates for human pancreatic α-amylase. Carbohydrate Research 339 (10): 1727–1737.

    Article  CAS  PubMed  Google Scholar 

  • Dao, T.M.A., A. Waget, P. Klopp, M. Serino, C. Vachoux, L. Pechere, and E. Sérée. 2011. Resveratrol increases glucose induced GLP-1 secretion in mice: A mechanism which contributes to the glycemic control. PLoS ONE 6 (6): e20700.

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  • De Rosa, S., B. Arcidiacono, E. Chiefari, A. Brunetti, C. Indolfi, and D.P. Foti. 2018. Type 2 diabetes mellitus and cardiovascular disease: Genetic and epigenetic links. Frontiers in Endocrinology 17 (9): 2.

    Article  Google Scholar 

  • Dixon, J.B., L.M. Chuang, K. Chong, S.C. Chen, G.W. Lambert, N.E. Straznicky, and W.J. Lee. 2013. Predicting the glycemic response to gastric bypass surgery in patients with type 2 diabetes. Diabetes Care 36 (1): 20–26.

    Article  CAS  PubMed  Google Scholar 

  • Giuberti, G., G. Rocchetti, and L. Lucini. 2020. Interactions between phenolic compounds, amylolytic enzymes and starch: An updated overview. Current Opinion in Food Science 31: 102–113.

    Article  Google Scholar 

  • Gong, L., D. Feng, T. Wang, Y. Ren, Y. Liu, and J. Wang. 2020. Inhibitors of α-amylase and α-glucosidase: Potential linkage for whole cereal foods on prevention of hyperglycemia. Food Science Nutrition 8 (12), 6320–6337.‏‏

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Igoumenidis, P.E., P. Zoumpoulakis, and V.T. Karathanos. 2018. Physicochemical interactions between rice starch and caffeic acid during boiling. Food Research International 109: 589–595.

    Article  CAS  PubMed  Google Scholar 

  • Inzucchi, S.E. 2002. Oral antihyperglycemic therapy for type 2 diabetes: Scientific review. JAMA 287 (3): 360–372.

    Article  CAS  PubMed  Google Scholar 

  • Iqbal, M., M.A. Qamar, T.H. Bokhari, M. Abbas, F. Hussain, N. Masood, and A. Nazir. 2017. Total phenolic, chromium contents and antioxidant activity of raw and processed sugars. Information Processing in Agriculture 4 (1): 83–89.

    Article  Google Scholar 

  • Jeyabal, P.V.S., M.B. Syed, M. Venkataraman, J.K. Sambandham, and D. Sakthisekaran. 2005. Apigenin inhibits oxidative stress- induced macromolecular damage in Nnitrosodiethylamine (NDEA)- induced hepatocellular carcinogenesis in Wistar albino rats. Molecular Carcinogenesis: Published in Cooperation with the University of Texas MD Anderson Cancer Center 44 (1): 11–20.

    Article  CAS  Google Scholar 

  • Johnston, K., P. Sharp, M. Clifford, and L. Morgan. 2005. Dietary polyphenols decrease glucose uptake by human intestinal Caco-2 cells. FEBS Letters 579 (7): 1653–1657.

    Article  CAS  PubMed  Google Scholar 

  • Kadam, U.S., S. Ghosh, S. De, P. Suprasanna, T.P. Devasagayam, and V. Bapat. 2008. Antioxidant activity in sugarcane juice and its protective role against radiation induced DNA damage. Food Chemistry 106 (3): 1154–1160.

    Article  CAS  Google Scholar 

  • Khurana, S., K. Venkataraman, A. Hollingsworth, M. Piche, and T. Tai. 2013. Polyphenols: Benefits to the cardiovascular system in health and in aging. Nutrients 5 (10): 3779–3827.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim, J.S., C.S. Kwon, and K.H. Son. 2000. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Bioscience, Biotechnology, and Biochemistry 64 (11): 2458–2461.

    Article  CAS  PubMed  Google Scholar 

  • Kumar, A., & S. Singh. 2020. The benefit of Indian jaggery over sugar on human health. In Dietary sugar, salt and fat in human health 2020 Jan 1 (pp. 347–359).‏

  • Kwon, Y.I., E. Apostolidis, and K. Shetty. 2008. Inhibitory potential of wine and tea against α-amylase and α- glucosidase for management of hyperglycemia linked to type 2 diabetes. Journal of Food Biochemistry 32 (1): 15–31.

    Article  CAS  Google Scholar 

  • Ledón, N., A. Casacó, V. Rodríguez, J. Cruz, R. González, and Z. Tolón. 2003. Antiinflammatory and analgesic effects of a mixture of fatty acids isolated and purified from sugarcane wax oil. Planta Medica 69: 367–9.

    Article  PubMed  Google Scholar 

  • Lee, J.S., S. Ramalingam, I.G. Jo, Y.S. Kwon, A. Bahuguna, Y.S. Oh, and M. Kim. 2018. Comparative study of the physicochemical, nutritional, and antioxidant properties of some commercial refined and non-centrifugal sugars. Food Research International 109: 614–625.

    Article  CAS  PubMed  Google Scholar 

  • Li, D.D., Y. Yang, Z.Y. Gao, L. Zhao, X. Yang, F. Xu, and J.B. Su. 2022. Sedentary lifestyle and body composition in type 2 diabetes. Diabetology & Metabolic Syndrome 14 (1): 1–11.

    Article  Google Scholar 

  • Lin, D., M. Xiao, J. Zhao, Z. Li, B. Xing, X. Li, and S. Chen. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21 (10): 1374.

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu, B., F. Zhong, W. Yokoyama, D. Huang, S. Zhu, and Y. Li. 2020. Interactions in starch cogelatinized with phenolic compound systems: Effect of complexity of phenolic compounds and amylose content of starch. Carbohydrate Polymers 247: 116667.

    Article  CAS  PubMed  Google Scholar 

  • Lo Piparo, E., H. Scheib, N. Frei, G. Williamson, M. Grigorov, and C. Chou. 2008. Flavonoids for controlling starch digestion: Structural requirements for inhibiting human α-amylase. Journal of Medicinal Chemistry 51 (12): 3555–3561.

    Article  CAS  PubMed  Google Scholar 

  • Loo, Alvin Eng Kiat., and Dejian Huang. 2007. Assay-guided fractionation study of α-amylase inhibitors from Garcinia mangostana pericarp. Journal of Agricultural and Food Chemistry 55 (24): 9805–9810.

    Article  CAS  PubMed  Google Scholar 

  • Lordan, S., T.J. Smyth, A. Soler-Vila, C. Stanton, and R. Ross. 2013. The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chemistry 141 (3): 2170–2176.

    Article  CAS  PubMed  Google Scholar 

  • Mandal, D., S. Tudu, S.R. Mitra, and G.C. De. 2006. Effect of common packing materials on keeping quality of sugarcane jaggery during monsoon season. Sugar Tech 8: 137–142.

    Article  Google Scholar 

  • Matheus, A.S.D.M., L.R.M. Tannus, R.A. Cobas, C.C.S. Palma, C.A. Negrato, and M.D.B. Gomes. 2013. Impact of diabetes on cardiovascular disease: An update. International Journal of Hypertension 2013: 653789.

    Article  PubMed  PubMed Central  Google Scholar 

  • Maurício Duarte-Almeida, J., A.V. Novoa, A.F. Linares, F.M. Lajolo, and M. Inés Genovese. 2006. Antioxidant activity of phenolics compounds from sugar cane (Saccharum officinarum L.) juice. Plant Foods for Human Nutrition 61 (4): 187–192.

    Article  PubMed  Google Scholar 

  • Melzig, M.F., and I. Funke. 2007. Pflanzliche alpha-Amylasehemmer–eine Möglichkeit zur Phytotherapie bei Diabetes mellitus Typ II? Wiener Medizinische Wochenschrift 157 (13): 320–324.

    Article  PubMed  Google Scholar 

  • Miao, M., H. Jiang, B. Jiang, Y. Li, S. Cui, and Z. Jin. 2013. Elucidation of structural difference in theaflavins for modulation of starch digestion. Journal of Functional Foods 5 (4): 2024–2029.

    Article  CAS  Google Scholar 

  • Nayaka, M.H., U.V. Sathisha, M.P. Manohar, K.B. Chandrashekar, and S.M. Dharmesh. 2009. Cytoprotective and antioxidant activity studies of jaggery sugar. Food Chemistry 115 (1): 113–118.

    Article  Google Scholar 

  • Nayaka, M.A.H., L. Chikkappaiah, K.S. Venkatesh, B.S. Gunashree, and S. Sudharshan. 2018. Evaluation of bioactivity of jaggery prepared using plant mucilage as clarificant. Asian Journal of Pharmaceutical and Clinical Research 11: 294–299.

    Article  Google Scholar 

  • Oyedemi, S., T. Koekemoer, G. Bradley, M. van de Venter, and A. Afolayan. 2013. In vitro antihyperglycemia properties of the aqueous stem bark extract from Strychnos henningsii (Gilg). International Journal of Diabetes in Developing Countries 33 (2): 120–127.

    Article  Google Scholar 

  • Papoutsis, K., J. Zhang, M. Bowyer, N. Brunton, E. Gibney, and J. Lyng. 2021. Fruit, vegetables, and mushrooms for the preparation of extracts with α-amylase and α-glucosidase inhibition properties: A review. Food Chemistry 338: 128119.

    Article  CAS  PubMed  Google Scholar 

  • Petrie, J.R., T.J. Guzik, and R.M. Touyz. 2018. Diabetes, hypertension, and cardiovascular disease: Clinical insights and vascular mechanisms. Canadian Journal of Cardiology 34 (5): 575–584.

    Article  PubMed  Google Scholar 

  • Pierson, J.T., R. Dietzgen, P. Shaw, S. Roberts- Thomson, G. Monteith, and M. Gidley. 2012. Major Australian tropical fruits biodiversity: Bioactive compounds and their bioactivities. Molecular Nutrition & Food Research 56 (3): 357–387.

    Article  CAS  Google Scholar 

  • Podsedek, A., I. Majewska, M. Redzynia, D. Sosnowska, and M. Koziołkiewicz. 2014. In vitro inhibitory effect on digestive enzymes and antioxidant potential of commonly consumed fruits. Journal of Agricultural and Food Chemistry 62 (20): 4610–4617.

    Article  CAS  PubMed  Google Scholar 

  • Poznyak, A., A.V. Grechko, P. Poggio, V. Myasoedova, V. Alfieri, and A. Orekhov. 2020. The diabetes mellitus–atherosclerosis connection: The role of lipid and glucose metabolism and chronic inflammation. International Journal of Molecular Sciences 21 (5): 1835.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ramamoorthy, P.K., and A. Bono. 2007. Antioxidant activity, total phenolic and flavonoid content of Morinda citrifolia fruit extracts from various extraction processes. Journal of Engineering Science and Technology 2 (1): 70–80.

    Google Scholar 

  • Ranilla, L.G., Y.I. Kwon, M. Genovese, F. Lajolo, and K. Shetty. 2008. Antidiabetes and antihypertension potential of commonly consumed carbohydrate sweeteners using in vitro models. Journal of Medicinal Food 11 (2): 337–348.

    Article  CAS  PubMed  Google Scholar 

  • Re, L., S. Barocci, C. Capitaini, C. Vivani, M. Ricci, L. Rinaldi, et al. 1999. Effects of some natural extracts on the acetylcholine release at the mouse neuromuscular junction. Pharmacological Research 39: 239–245.

    Article  CAS  PubMed  Google Scholar 

  • Sangeetha, R., and N. Vedasree. 2012. In vitro α-amylase inhibitory activity of the leaves of Thespesia populnea. International Scholarly Research Notices (2012).

  • Sonkawade, S.D., and G.R. Naik. 2015. In vitro evaluation of antioxidant properties of sugarcane extracts rich in dietary nucleotides. International Journal of Advanced Research 5: 243–250.

    CAS  Google Scholar 

  • Sun, L., F.J. Warren, and M. Gidley. 2019. Natural products for glycaemic control: Polyphenols as inhibitors of alpha-amylase. Trends in Food Science & Technology 91: 262–273.

    Article  CAS  Google Scholar 

  • Uma, P., R.S. Hariharan, V. Ramani, and V. Seshia. 1987. Glycaemic indices of different sugars. International Journal of Diabetes in Developing Countries 7: 78–82.

    Google Scholar 

  • Vengaiah, P.C., D. Ravindrababu, G.N. Murthy, and K.R. Prasad. 2013. Jaggery from Palmyrah palm (Borassus flabellifer L.). Present Status and Scope 12 (94): 714–717.

    Google Scholar 

  • Wali, J.A., S.M. Solon-Biet, T. Freire, and A.E. Brandon. 2021. Macronutrient determinants of obesity, insulin resistance and metabolic health. Biology 10 (4): 336.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu, Y., Q. Lin, Z. Chen, and H. Xiao. 2011. The interaction between tea polyphenols and rice starch during gelatinization. Food Science and Technology International 17 (6): 569–577.

    Article  CAS  PubMed  Google Scholar 

  • Zhu, F. 2015. Interactions between starch and phenolic compounds. Trends in Food Science & Technology 43 (2): 129–143.

    Article  CAS  Google Scholar 

  • Zhu, Y., W.H. Hsu, and J.H. Hollis. 2013. The impact of food viscosity on eating rate, subjective appetite, glycemic response and gastric emptying rate. PLoS ONE 8 (6): e67482.

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  • Zinjarde, S.S., S.Y. Bhargava, and A. Kumar. 2011. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary and Alternative Medicine 11 (1): 1–10.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yaaser Q. Almulaiky.

Ethics declarations

Conflict of interest

There is no conflict of interest, and it has not been considered for publication elsewhere.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file 1 (DOCX 18 kb)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ahmed, M.S.H., Kumar, D., Nayaka, M.A.H. et al. Effect of Sugarcane Jaggery Prepared Using Different Heat Treatment Methods on α-Amylase Activity. Sugar Tech 26, 207–214 (2024). https://doi.org/10.1007/s12355-023-01331-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-023-01331-7

Keywords

Navigation