Abstract
The principal desirable characteristics of the yeasts used in order to maintain the yield and the efficiency high during the process of ethanol production are mainly thermotolerance, Killer activity and high resistance to inhibitor compounds like acetic acid. Yeast represents the most important factor to achieve an efficient conversion process of the different raw materials used to produce ethanol like sorghum and sugarcane juice or their lignocellulosic materials. The aim of this work was to isolate and select native yeasts from sweet sorghum juice with thermotolerance and resistance to toxic compounds like acetic acid, furfural and 5-hydroxymethylfurfural, capable of producing high ethanol concentrations. The results obtained showed that native yeast Pichia kudriavzevii ITV-S42 exhibit thermotolerance at 40 °C, positive Killer activity, tolerant to acetic acid up to 24 g/L initial concentration, and tolerant to furfural and 5-hydroxymethylfurfural up to 1 g/L for both compounds. An ethanol productivity and yield of 0.54 g/Lh and 0.376 g ethanol/g glucose, respectively, were obtained. These results show that the desirable characteristics of Pichia kudriavzevii ITV-S42 could be interesting for their implementation in first and second generation ethanol process production.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig1_HTML.jpg)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig3_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig4_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig5_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig6_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12355-021-01040-z/MediaObjects/12355_2021_1040_Fig7_HTML.png)
Similar content being viewed by others
References
Almodares, A., and M.R. Hadi. 2009. Production of bioethanol from sweet sorghum: A review. African Journal of Biotechnology 9: 77–780. https://doi.org/10.5897/AJAR.9000567.
Arora, R., S. Behera, N.K. Sharma, and S. Kumar. 2015. A new search for thermotolerant yeasts, its characterization and optimization using response surface methodology for ethanol production. Frontiers in Microbiology 6: 889. https://doi.org/10.3389/fmicb.2015.00889.
Ballesteros, M., J.M. Oliva, M.J. Negro, P. Manzanares, and I. Ballesteros. 2004. Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875. Process Biochemistry 39: 1843–1848. https://doi.org/10.1016/j.procbio.2003.09.011.
Chamnipa, N., S. Thanonkeo, P. Klanrit, and P. Thanonkeo. 2018. The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production. Brazilian Journal of Microbiology 49: 378–391. https://doi.org/10.1016/j.bjm.2017.09.002.
Charoensopharat, K., P. Thanonkeo, S. Thanonkeo, and M. Yamada. 2015. Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing. Anton Van Leeuwenhoek 108: 173–190. https://doi.org/10.1007/s10482-015-0476-5.
Costa, D.A., C.J. de Souza, P.S. Costa, M.Q. Rodrigues, A.F. dos Santos, M.R. Lopes, H.L. Genier, W.B. Silveira, and L.G. Fietto. 2014. Physiological characterization of thermotolerant yeast for cellulosic ethanol production. Applied Microbiology and Biotechnology 98: 3829–3840. https://doi.org/10.1007/s00253-014-5580-3.
D’Amore, T., C.J. Panchal, I. Russell, and G.G. Stewart. 1990. A study of ethanol tolerance in yeast. Critical Reviews in Microbiology 9: 287–304. https://doi.org/10.3109/07388558909036740.
Díaz-Nava, L.E., N. Montes-Garcia, J.M. Domínguez, and M.G. Aguilar-Uscanga. 2017. Effect of carbon sources on the growth and ethanol production of native yeast Pichia kudriavzevii ITV-S42 isolated from sweet sorghum juice. Bioprocess and Biosystems Engineering 40: 1069–1077. https://doi.org/10.1007/s00449-017-1769-z.
Delgenes, J.P., M.C. Escare, J.M. Laplace, R. Moletta, and J.M. Navarro. 1998. Biological production of industrial chemicals, i.e. xylitol and ethanol, from lignocelluloses by controlled mixed culture systems. Industrial Crops and Products 7: 101–111. https://doi.org/10.1016/S0926-6690(97)00038-1.
Felipe, M.G.A., I.M. Mancilha, S.S. Silva, and M. Vitolo. 1997. Environmental parameters affecting xylitol production from sugarcane bagasse hemicellulosic hydrolyzate by Candida guilliermondii. Journal of Industrial Microbiology and Biotechnology 18: 251–254. https://doi.org/10.1038/sj.jim.2900374.
Hernández, M.T., T. Sais and O. Sánchez. 1986. Microbiología de la Producción Azucarera. Producciones Microbianas Derivadas. Edición UCLV, Santa Clara, Cuba.
Hernandez, A., A. Martin, M.G. Cordoba, and M.J. Benito. 2008. Determination of Killer activity in yeasts isolated from the elaboration of seasoned green table olives. International Journal of Food Microbiology 121: 178–188. https://doi.org/10.1016/j.ijfoodmicro.2007.11.044.
Ingram, L.O., and T.M. Buttke. 1985. Effects of alcohols on microorganisms. Advances in Microbial Physiology 25: 253–300. https://doi.org/10.1016/S0065-2911(08)60294-5.
Koutinas, M., M. Patsalou, S. Stavrinou, and I. Vyrides. 2016. High temperature alcoholic fermentation of orange peel by the newly isolated thermotolerant Pichia kudriavzevii KVMP10. Letters in Applied Microbiology 62: 75–83. https://doi.org/10.1111/lam.12514.
Lee, C., T. Kodama, and T. Yamakawa. 1993. Rapid growth of thermotolerant yeast on palm oil. World Journal of Microbiology and Biotechnology 9: 187–190. https://doi.org/10.1007/BF00327834.
Martin, C., M. Galbe, L.J. Jönsson, and N.O. Nilvebrant. 2007. A study of three strategies for improving the fermentability of sugarcane bagasse hydrolysates for fuel ethanol production. International Sugar Journal 109: 33–39.
Monod, J. 1949. The growth of bacterial cultures. Annual Review of Microbiology 3: 371. https://doi.org/10.1146/annurev.mi.03.100149.002103.
Morita, T.A., S.S. Silva, and M.G.A. Felipe. 2000. Effects of initial pH on biological synthesis of xylitol using xylose-rich hydrolysate. Applied Microbiology and Biotechnology 84–86: 751–759. https://doi.org/10.1385/ABAB:84-86:1-9:751.
Ortiz-Muñiz, B., M.G. Aguilar-Uscanga, O. Carvajal-Zarrabal, and B. Torrestiana-Sánchez. 2010. Kinetic study on ethanol production using Saccharomyces cerevisiae ITV-01 yeast isolated from sugarcane molasses. Journal of Chemical Technology and Biotechnology 85: 1361–1367. https://doi.org/10.1002/jctb.2441.
Ortiz-Muñiz, B. 2010b. Estudio de la fisiología de Saccharomyces cerevisiae ITV-01 y su deficiente respiratoria para la producción de etanol. Doctoral thesis. Instituto Tecnológico de Veracruz .Veracruz, México.
Ortiz-Zamora, O., M.G. Aguilar-Uscanga, R. Cortés-García, J. Gómez-Rodríguez, and M. Ramírez-Lepe. 2007. Isolation and Selection of ethanol-resistant and osmotolerant yeasts from regional agricultural sources in Mexico. Journal of Food Process Engineering 32: 775–786. https://doi.org/10.1111/j.1745-4530.2008.00244.x.
Panchal, C.J. 1990. Yeast strain selection. Biotechnology and bioprocessing series. New York: Marcel Dekker Inc.
Piper, P.W. 1995. The heat-shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. FEMS Yeast Research 134: 121–127. https://doi.org/10.1111/j.1574-6968.1995.tb07925.x.
Polonelli, L., W. Magliani, T. Ciociola, L. Giovato, and S. Conti. 2011. From Pichia anomala Killer toxin through Killer antibodies to Killer peptides for a comprehensive anti-infective strategy. Antonie Van Leeuwenhoek 99: 35–41. https://doi.org/10.1007/s10482-010-9496-3.
Rahman, K., K. Ismail, and N. Najimudin. 2021. Growth of thermotolerant Pichia kudriavzevii UniMAP 3–1 strain for ethanol production using xylose and glucose at different fermentation temperatures. IOP Conference Series: Earth and Environmental Science. 765: 012107. https://doi.org/10.1088/1755-1315/765/1/012107.
Rangel, L.E. 2006. Efectos de los requerimientos nutricionales y de productos tóxicos presentes en hidrolizados de lignocelulósicos sobre la producción de xylitol por la capa IEC5-ITV. Masters thesis. Instituto Tecnológico de Veracruz. Veracruz. México.
Ratnavathi, C.V., S.K. Chakravarthy, U.D. Chavan, V.V. Komala, and J.V. Patil. 2011. Sweet sorghum as feedstock for bio-fuel production: A review. Sugar Tech 13: 399–440. https://doi.org/10.1007/s12355-011-0112-2.
Rosini, G. 1983. The occurrence of killer characters in yeasts. Canadian Journal of Microbiology 29: 1462–1464. https://doi.org/10.1139/m83-224.
Rouhollah, H., E. Giti, N. Iraj, and A. Sorah. 2007. Mixed sugar fermentation by Pichia stipitis, Saccharomyces cerevisiae, and an isolated xylose fermenting Kluyveromyces marxianus and their co-cultures. African Journal of Biotechnology 6: 1110–1114. https://doi.org/10.4314/ajb.v6i9.57123.
Sohn, H.Y., I. Jin, W. Park, and J.H. Seu. 1994. The fermentation characteristics of newly selected thermotolerant yeasts at high temperature. Journal of Microbiology and Biotechnology 4: 222–229.
Strehaiano, P. 1984. Phénomenes d’ inhibition et fermentation alcoolique, Tesis Doctoral. I. P. N. Toulouse, Francia.
Suzuki, C., Y. Ando, and S. Machida. 2001. Interaction of SMKT, a Killer toxin produced by Pichia farinosa, with the yeast cell membranes. Yeast 18: 1471–1478. https://doi.org/10.1002/yea.791.
Vázquez, H.J., and O. Dacosta. 2007. Fermentación alcohólica: Una opción para la producción de energía renovable a partir de desechos agrícolas. Ingeniería Investigación y Tecnología 8: 249–259.
Acknowledgements
Authors acknowledge the economic support from the National Council of Science and Technology (CONACyT) through the doctoral grant of Libia Diaz-Nava and also the critical reading of Dulce Maria Barradas-Dermitz MSc. and Patricia Margaret Hayward-Jones MSc.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Díaz-Nava, L.E., Aguilar-Uscanga, M.G., Ortiz-Muñiz, B. et al. Acetic Acid-Tolerant Native Yeast Pichia kudriavzevii ITV-S42 Isolated from Sweet Sorghum Juice for Ethanol Production. Sugar Tech 24, 576–584 (2022). https://doi.org/10.1007/s12355-021-01040-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12355-021-01040-z