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Beneficial Linkages of Endophytic Burkholderia anthina MYSP113 Towards Sugarcane Growth Promotion

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Abstract

Microbial agents are the best alternative to the chemical fertilizers that promote the plant growth in an eco-friendly manner. This study was intended to characterize Burkholderia anthina strain MYSP113 and to evaluate its mechanisms linked to sugarcane growth promotion. The morphological, biochemical, and molecular characterization (gyrB and recA) results identified the bacteria as B. anthina. To determine the plant bacterial association, MYSP113 strain was tagged with a plasmid-bear green fluorescent protein (gfp) gene, and the tagged strain was inoculated to the sugarcane (variety GXB-9), and bacterial colonization analyzed by confocal laser fluorescence microscopy. The results revealed that strain MYSP113 had root-colonizing abilities, and colonization was detected at the root and leaves maturation zones. Bacteria entered the roots via cracks at the surfacing site of the roots and disrupted epidermis. Bacterial cells were observed in the substomatal chambers of leaves. Furthermore, to evaluate the mechanisms related to sugarcane growth promotion and bacterial competence in soil and plant, greenhouse experiment performed, and bacterial competence, plant growth parameters, and phytoenzymes, and hormones were assessed with or without MYSP113. We examined bacterial competence in soil and plant via qPCR and direct plant count at 45 and 90 DAI, and results recommended that strain MYSP113 has the ability to colonize in sugarcane rhizosphere and in the aerial part of the plant. Furthermore, strain MYSP113 significantly induced the plant fresh weight, dry weight, and chlorophyll content of sugarcane seedlings at 45 and 90 days after inoculation. Additionally, strain MYSP113 triggered chitinase, cellulose, indole acetic acid, and abscisic acid production in the plant roots and aerial parts, and these results signifying that bacterial inoculation modulates the plant metabolism and boost the health. The present study concluded that strain MYSP113 has beneficial linkages with the sugarcane plant, and it would be utilized as a bioinoculant in future.

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Acknowledgements

National Natural Science Foundation of China, China Postdoctoral Foundation, Guangxi Innovation Term of Modern Agriculture Technology, and Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences.

Funding

This research was supported jointly by National Natural Science Foundation of China (31471449), Guangxi Funds for Bagui Scholars and Distinguished Experts (TF2013-03), Natural Science Foundation of Guangxi Province (2014GXNSFBA118085, 2016GXNSFAA380126), Project funded by China Postdoctoral Foundation, Guangxi Innovation Term of Modern Agriculture Technology (gjnytxgxcxtd-03-01), Guangxi R&D Program (GKN14121008-2-1, GKG1598006-1-1, GuiKeAD17195100).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, 174 Daxue Road, Nanning, 530007, Guangxi, China

    Mukesh Kumar Malviya, Manoj Kumar Solanki, Chang-Ning Li, Rajesh Kumar Singh & Yang-Rui Li

  2. Department of Biotechnology, Mandalay Technology University, Mandalay, Myanmar

    Reemon Htun

  3. Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China

    Pratiksha Singh, Li-Tao Yang & Yang-Rui Li

  4. Department of Food Quality and Safety, Institute for Post-harvest and Food Sciences, Agricultural Research Organization, The VolcaniCenter, HaMaccabim Road 68, P.O.B 15159, 7528809, Rishon LeZion, Israel

    Manoj Kumar Solanki

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  1. Mukesh Kumar Malviya
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Malviya, M.K., Solanki, M.K., Li, CN. et al. Beneficial Linkages of Endophytic Burkholderia anthina MYSP113 Towards Sugarcane Growth Promotion. Sugar Tech 21, 737–748 (2019). https://doi.org/10.1007/s12355-019-00703-2

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