Abstract
Metabolic changes in the contents of sucrose and hexoses in relation to activities of the principal enzymes involved in accumulation of sucrose in internodal storage tissue have been investigated in four sugarcane genotypes varying in maturity behaviour viz. Sel 69/01, Sel 943/98 (both early maturing) and CoS 8436, Sel 17/00 (both mid-late maturing) at different physiological stages of crop growth. In storage tissue, the higher rate of decline in the activity of soluble acid invertase (pH 5.5) with age of cane resulted in faster rate of sucrose accumulation in early maturing sugarcane genotypes and was related to higher sink strength of these genotypes. Unlike soluble acid invertase, the activity of soluble neutral invertase increased with cane maturation in each of the genotypes. The wall-bound acid invertase (pH 5.5) showed maximum activity at stem elongation stage. Mid-late maturing genotypes showed higher activity of this enzyme than early maturing genotypes. No relationship was observed between the activities of sucrose synthase, sucrose phosphate synthase and maturity behaviour of the genotypes.
Similar content being viewed by others
References
Bamber ING, Bonnett GD, Spillman MF, Hevitt ML, Jackson J (2008) Increasing sucrose accumulation in sugarcane by manipulating leaf extension and photosynthesis with irrigation. Aus. J. Agri. Research 59: 13–26.
Batta SK, Singh R (1986) Sucrose metabolism in sugarcane grown under varying climatic conditions: Synthesis and storage of sucrose in relation to the activities of sucrose synthase, sucrose-phosphate synthase and invertase. Phytochemistry, 25: 2431–2437.
Batta SK, Kaur K, Singh R (1995) Synthesis and storage of sucrose in relation to activities of its metabolizing enzymes in sugarcane cultivars differing in maturity. J. Plant Biochem. Biotech. 4: 17–22.
Batta SK, Kaur S, Mann APS (2002) Sucrose accumulation and maturity behaviour in sugarcane is related to invertase activities under subtropical conditions. Sugarcane International Jan.–Feb., 10–13.
Batta SK, Benkush Sital JS, Mann APS (2007a) Internodal soluble invertase isoenzymes in relation to sucrose storage and maturity behaviour in sugarcane cultivars. Sugar Tech 9: 43–50.
Batta SK, Ubhi MK, Sandhu SK, Uppal SK (2007b) Enzymatic regulation of sucrose synthesis, transport and accumulation in sugarcane cultivars differing in maturity. Sugar Tech 9: 263–270.
Bosch S, Grof CPL, Botha FC (2004) Expression of neutral invertase in sugarcane. Plant Sci 166: 1125–1133.
Botha FC, Black KG (2000) Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Aust. J. Plant Physiol. 27: 81–85.
Gayler KR, Glasziou KT (1972) Physiological functions of acid and neutral invertases in growth and sugar storage in sugarcane. Physiol. Plant. 27: 25–31.
Glasziou KT, Gayler KR (1972) Storage of sugars in stalks of sugarcane. The Bot. Rev. 38: 471–490.
Grof CPL, Albertson PL, Bursle J, Perroux JM, Bonnett GD, Manners JM (2007) Sucrose-phosphate synthase, a biochemical marker of high sucrose accumulation in sugarcane. Crop Sci. 47: 1530–1539.
Gutierrez MFA, Arias CC, Dendooven L, Mendez SR, Rodriguez MMA, Ochoa AN, Bellow PLA (2002) Enzymatic regulation in the saccharose accumulation in sugarcane (Saccharum spp.) plants. Agrociencia, 36: 411–419.
Hongmei M, Henrick HA, Robert P, Paul HM (2000) Metabolic engineering of invertase activities in different sub-cellular compartments affects sucrose accumulation in sugarcane cells. Aust. J. Plant Physiol., 27: 1021–1030.
Hussain A, Khan ZI, Ghanfoor MY, Ashraf M, Parveen R, Rashid MH (2004) Sugarcane, sugar metabolism and some abiotic stresses. Int. J. Agri. Biol., 6: 732–742.
Lingle SE (1997) Seasonal internode development and sugar metabolism in sugarcane. Crop Sci. 37: 1222–1227.
Lingle SE (1999) Sugar metabolism during growth and development in sugarcane internodes. Crop Sci. 39: 480–486.
Lingle SE, Smith RC (1991) Sucrose metabolism related to growth and ripening in sugarcane internodes. Crop Sci. 31: 172–177.
Mao L, Que F, Wang G (2006) Sugar metabolism and involvement of enzymes in sugarcane (Saccharum officinarum L.) stems during storage. Food Chemistry, 98: 338–342.
Moore PH (1995) Temporal and spatial regulation of sucrose accumulation in the sugarcane stem. Aust. J. Plant Physiol., 22:661–679.
Moore PH, Botha FC, Furbank RT, Grof CPL (1997) Potential for overcoming physio-biochemical limits to sucrose accumulation. pp. 141–155. In: Keating BA and Wilson JR, eds. Intensive Sugarcane Production. Meeting the Challenges 2000. CAB International, Wallingford, U.K.
Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem., 153: 375–380.
Prado FE, Fleischmacher OL, Vattuone MA, Sampietro AR (1982) Cell wall invertases of sugarcane. Phytochemistry 21: 2825–2828.
Rae AL, Grof CPL, Casu RE, Bonnett GD (2005) Sucrose accumulation in the sugarcane stem: pathways and control points for transport and compartmentation. Field Crops Research. 92: 159–168.
Roe JH (1934) A colorimetric method for determination of fructose in blood and urine. J. Biol. Chem., 107: 15–22.
Rohwer JM, Botha FC (2001) Analysis of sucrose accumulation in the sugarcane culm on the basis of in vitro kinetic data. Biochem. J. 358: 437–445.
Rose S, Botha FC (2000) Distribution patterns of neutral invertase and sugar content in sugarcane internodal tissues (2000). Plant Physiol. Biochem., 38: 819–824.
Sachdeva M, Mann APS, Batta SK (2003) Sucrose metabolism and expression of key enzyme activities in low and high sucrose storing sugarcane genotypes. Sugar Tech. 5: 265–271.
Schafer WE, Rohwer JM, Botha FC (2004) A kinetic study of sugarcane sucrose synthase. Eur. J. Biochem. 271: 3971–3977.
Sehtiya HL, Dendsay JPS, Dhawan AK (1995) Internodal invertases and stalk maturity in sugarcane. J. Agri. Sci. 116: 239–243.
Uys L, Botha FC, Hofmeyr SJH, Rohwer MJ (2007) Kinetic model of sucrose accumulation in maturing sugarcane culm tissue. Phytochemistry, 68: 2375–2392.
Vattuone MA, Fleischmacher OL, Prado FE, Vinals AF, Sampietro AR (1983) Localisation of invertase activities in Ricinus communis leaves. Phytochemistry 22: 1361–1365.
Vorster DJ, Botha FC (1998) Partial purification and characterization of sugarcane neutral invertase. Phytochemistry, 49(3): 651–655.
Vorster DJ, Botha FC (1999) Sugarcane internodal invertases and tissue maturity. J Plant Physiol. 155: 470–476.
Zhu YJ, Albert HH, Moore PH (2000) Differential expression of soluble acid invertase (SAI) genes correlates to differences in sucrose accumulation in sugarcane. Aust. J. Plant Physiol. 27: 193–199.
Zhu YJ, Komor E, Moore PH (1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol. 115: 609–616.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Batta, S.K., Pant, N.C., Thind, K.S. et al. Sucrose accumulation and expression of enzyme activities in early and mid-late maturing sugarcane genotypes. Sugar Tech 10, 319–326 (2008). https://doi.org/10.1007/s12355-008-0057-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12355-008-0057-2