Abstract
Drought is one of the main environmental stresses affecting the quality and quantity of sesame production worldwide. The present study was conducted to investigate the effect of drought stress and subsequent re-watering on physiological, biochemical, and molecular responses of two contrasted sesame genotypes (susceptible vs. tolerant). Results showed that plant growth, photosynthetic rate, stomatal conductance, transpiration rate, and relative water content were negatively affected in both genotypes during water deficit. Both genotypes accumulated more soluble sugars, free amino acids, and proline and exhibited an increased enzyme activity for peroxidase, catalase, superoxide dismutase, and pyruvate dehydrogenase in response to drought damages including increased lipid peroxidation and membrane disruption. However, the tolerant genotype revealed a more extended root system and a more efficient photosynthetic apparatus. It also accumulated more soluble sugars (152%), free amino acids (48%), proline (75%), and antioxidant enzymes while showing lower electrolyte leakage (26%), lipid peroxidation (31%), and starch (35%) content, compared to the susceptible genotype at severe drought. Moreover, drought-related genes such as MnSOD1, MnSOD2, and PDHA-M were more expressed in the tolerant genotype, which encode manganese-dependent superoxide dismutase and the alpha subunit of pyruvate dehydrogenase, respectively. Upon re-watering, tolerant genotype recovered to almost normal levels of photosynthesis, carboxylation efficiency, lipid peroxidation, and electrolyte leakage, while susceptible genotype still suffered critical issues. Overall, these results suggest that a developed root system and an efficient photosynthetic apparatus along with the timely and effective accumulation of protective compounds enabled the tolerant sesame to withstand stress and successfully return to a normal growth state after drought relief. The findings of this study can be used as promising criteria for evaluating genotypes under drought stress in future sesame breeding programs.
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References
Abid M, Ali S, Qi LK, Zahoor R, Tian Z, Jiang D, Snider JL, Dai T (2018) Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L). Sci Rep 8(1):1–15. https://doi.org/10.1038/s41598-018-21441-7
Acevedo RM, Avico EH, González S, Salvador AR, Rivarola M, Paniego N, Nunes-Nesi A, Ruiz OA, Sansberro PA (2019) Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Planta 250(2):445–462. https://doi.org/10.1007/s00425-019-03178-3
Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology. Academic Press, Cambridge, Massachusetts, pp 121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Agurla S, Gahir S, Munemasa S, Murata Y, Raghavendra AS (2018) Mechanism of stomatal closure in plants exposed to drought and cold stress. In: Iwaya-Inoue M, Sakurai M, Uemura M (eds) Survival Strategies in Extreme Cold and Desiccation. Advances in Experimental Medicine and Biology. Springer, Singapore, pp 215–232. https://doi.org/10.1007/978-981-13-1244-1_12
Amoah JN, Ko CS, Yoon JS, Weon SY (2019) Effect of drought acclimation on oxidative stress and transcript expression in wheat (Triticum aestivum L.). J Plant Interact 14(1):492–505. https://doi.org/10.1080/17429145.2019.1662098
Anjum NA, Sharma P, Gill SS, Hasanuzzaman M, Khan EA, Kachhap K, Mohamed AA, Thangavel P, Devi GD, Vasudhevan P (2016) Catalase and ascorbate peroxidase—representative H2O2-detoxifying heme enzymes in plants. Environ Sci Pollut Res 23(19):19002–19029. https://doi.org/10.1007/s11356-016-7309-6
Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59(2):206–216. https://doi.org/10.1016/j.envexpbot.2005.12.006
Baghery MA, Kazemitabar SK, Dehestani A, Mehrabanjoubani P, Najafi Zarini H (2022a) Evaluation of drought tolerance in sesame (Sesamum indicum L.) genotypes using germination traits and indices under drought conditions. J Iran Plant Ecophysiol Res 16(64):37–54
Baghery MA, Kazemitabar SK, Dehestani A, Mehrabanjoubani P, Najafi Zarrini H (2022b) Assessment of agro-morphological traits and yield-based tolerance indices in sesame (Sesamum indicum L.) genotypes under drought stress. Indian J Genet Plant Breed 82(03):324–332
Bajji M, Kinet J-M, Lutts S (2002) The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul 36(1):61–70. https://doi.org/10.1023/A:1014732714549
Bates LS, Waldren RP, Teare I (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207. https://doi.org/10.1007/BF00018060
Batista-Silva W, Heinemann B, Rugen N, Nunes-Nesi A, Araújo WL, Braun HP, Hildebrandt TM (2019) The role of amino acid metabolism during abiotic stress release. Plant Cell Environ 42(5):1630–1644. https://doi.org/10.1111/pce.13518
Bayat H, Moghadam AN (2019) Drought effects on growth, water status, proline content and antioxidant system in three Salvia nemorosa L. cultivars. Acta Physiol Plant 41(9):149. https://doi.org/10.1007/s11738-019-2942-6
Bedigian D (2010) Introduction: History of the cultivation and use of sesame. In: Bedigian D (ed) Sesame: the genus Sesamum. CRC Press, Boca Raton, pp 1–32. https://doi.org/10.1201/b13601
Berta M, Giovannelli A, Caparrini S, Racchi ML (2005) Expression of antioxidant genes in relation to water deficit in cambium and leaves of poplar. J Plant Interact 1(4):223–227. https://doi.org/10.1080/17429140601032767
Bolouri-Moghaddam MR, Le Roy K, Xiang L, Rolland F, Van den Ende W (2010) Sugar signalling and antioxidant network connections in plant cells. FEBS J 277(9):2022–2037. https://doi.org/10.1111/j.1742-4658.2010.07633.x
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Chen J, Zhao X, Zhang Y, Li Y, Luo Y, Ning Z, Wang R, Wang P, Cong A (2019) Effects of drought and rehydration on the physiological responses of Artemisia halodendron. Water 11(4):793. https://doi.org/10.3390/w11040793
Chintakovid N, Maipoka M, Phaonakrop N, Mickelbart MV, Roytrakul S, Chadchawan S (2017) Proteomic analysis of drought-responsive proteins in rice reveals photosynthesis-related adaptations to drought stress. Acta Physiol Plant 39(10):240. https://doi.org/10.1007/s11738-017-2532-4
Cooney RV, Custer LJ, Okinaka L, Franke AA (2001) Effects of dietary sesame seeds on plasma tocopherol levels. Nutr Cancer 39(1):66–71. https://doi.org/10.1207/S15327914nc391_9
Coutinho FS, Dos Santos DS, Lima LL, Vital CE, Santos LA, Pimenta MR, Da Silva JC, Ramos JRLS, Mehta A, Fontes EPB (2019) Mechanism of the drought tolerance of a transgenic soybean overexpressing the molecular chaperone BiP. Physiol Mol Biol Plants 25(2):457–472. https://doi.org/10.1007/s12298-019-00643-x
Davey M, Stals E, Panis B, Keulemans J, Swennen R (2005) High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 347(2):201–207. https://doi.org/10.1016/j.ab.2005.09.041
Desoky ESM, Alharbi K, Rady MM, Elnahal AS, Selem E, Arnaout SM, Mansour E (2023) Physiological, biochemical, anatomical, and agronomic responses of sesame to exogenously applied polyamines under different irrigation regimes. Agron 13(3):875. https://doi.org/10.3390/agronomy13030875
Dhindsa RS (1982) Inhibition of protein synthesis by products of lipid peroxidation. Phytochemistry 21(2):309–313. https://doi.org/10.1016/S0031-9422(00)95257-9
Dossa K, Li D, Wang L, Zheng X, Liu A, Yu J, Wei X, Zhou R, Fonceka D, Diouf D (2017) Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes. Sci Rep 7(1):1–14. https://doi.org/10.1038/s41598-017-09397-6
Du Z, Bramlage WJ (1992) Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40(9):1566–1570. https://doi.org/10.1021/jf00021a018
Du Y, Zhao Q, Chen L, Yao X, Zhang W, Zhang B, Xie F (2020) Effect of drought stress on sugar metabolism in leaves and roots of soybean seedlings. Plant Physiol Biochem 146:1–12. https://doi.org/10.1016/j.plaphy.2019.11.003
Erdal ŞÇ, Eyidoğan F, Ekmekçi Y (2021) Comparative physiological and proteomic analysis of cultivated and wild safflower response to drought stress and re-watering. Physiol Mol Biol Plants 27(2):281–295. https://doi.org/10.1007/s12298-021-00934-2
Fàbregas N, Fernie AR (2019) The metabolic response to drought. J Exp Bot 70(4):1077–1085. https://doi.org/10.1093/jxb/ery437
Fahad S, Ullah A, Ali U, Ali E, Saud S, Hakeem KR, Alharby H, Sabagh AEL, Barutcular C, Kamran M (2019) Drought tolerance in plantsrole of phytohormones and scavenging system of ROS. In: Hasanuzzaman M, Fujita M, Oku H, Islam MT (eds) Plant Tolerance to Environmental Stress. CRC Press, Boca Raton, pp 103–114. https://doi.org/10.1201/9780203705315
Fatland BL, Nikolau BJ, Wurtele ES (2005) Reverse genetic characterization of cytosolic acetyl-CoA generation by ATP-citrate lyase in Arabidopsis. Plant Cell 17(1):182–203. https://doi.org/10.1105/tpc.104.026211
Flexas J, Barbour MM, Brendel O, Cabrera HM, Carriquí M, Díaz-Espejo A, Douthe C, Dreyer E, Ferrio JP, Gago J (2012) Mesophyll diffusion conductance to CO2: an unappreciated central player in photosynthesis. Plant Sci 193:70–84. https://doi.org/10.1016/j.plantsci.2012.05.009
Forlani G, Trovato M, Funck D, Signorelli S (2019) Regulation of Proline Accumulation and Its Molecular and Physiological Functions in Stress Defence. In: Hossain MA, Kumar V, Burritt DJ, Fujita M, Mäkelä PSA (eds) Osmoprotectant-Mediated Abiotic Stress Tolerance in Plants. Springer, Cham, pp 73–97. https://doi.org/10.1007/978-3-030-27423-8_3
Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I occurrence in higher plants. Plant Physiol 59(2):309–314. https://doi.org/10.1104/pp.59.2.309
Good AG, Zaplachinski ST (1994) The effects of drought stress on free amino acid accumulation and protein synthesis in Brassica napus. Physiol Plant 90(1):9–14. https://doi.org/10.1111/j.1399-3054.1994.tb02185.x
Gopika K, Ratnakumar P, Guhey A, Manikanta CL, Pandey BB, Ramya K, Rathnakumar A (2022) Physiological traits and indices to identify tolerant genotypes in sesame (Sesamum indicum L.) under deficit soil moisture condition. Plant Physiol Rep 27:744–754. https://doi.org/10.1007/s40502-022-00701-9
Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, Von Korff M, Varshney RK, Graner A, Valkoun J (2009) Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot 60(12):3531–3544. https://doi.org/10.1093/jxb/erp194
Hasan MM-U, Ma F, Prodhan ZH, Li F, Shen H, Chen Y, Wang X (2018) Molecular and physio-biochemical characterization of cotton species for assessing drought stress tolerance. Int J Mol Sci 19(9):2636. https://doi.org/10.3390/ijms19092636
Hassanzadeh M, Asghari A, Jamaati-e-Somarin S, Saeidi M, Zabihi-e-Mahmoodabad R, Hokmalipour S (2009) Effects of water deficit on drought tolerance indices of sesame (Sesamum indicum L.) genotypes in Moghan region. Res J Environ Sci 3(1):116–121. https://doi.org/10.3923/rjes.2009.116.121
Hildebrandt TM (2018) Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response. Plant Mol Biol 98(1–2):121–135. https://doi.org/10.1007/s11103-018-0767-0
Hinman LM, Blass JP (1981) An NADH-linked spectrophotometric assay for pyruvate dehydrogenase complex in crude tissue homogenates. J Biol Chem 256(13):6583–6586. https://doi.org/10.1016/S0021-9258(19)69029-0
Hu L, Xie Y, Fan S, Wang Z, Wang F, Zhang B, Li H, Song J, Kong L (2018) Comparative analysis of root transcriptome profiles between drought-tolerant and susceptible wheat genotypes in response to water stress. Plant Sci 272:276–293. https://doi.org/10.1016/j.plantsci.2018.03.036
Huang T, Jander G (2017) Abscisic acid-regulated protein degradation causes osmotic stress-induced accumulation of branched-chain amino acids in Arabidopsis thaliana. Planta 246(4):737–747. https://doi.org/10.1007/s00425-017-2727-3
Islam F, Gill RA, Ali B, Farooq MA, Xu L, Najeeb U, Zhou W (2016) Sesame. In: Gupta SK (ed) Breeding Oilseed Crops for Sustainable Production. Academic Press, Cambridge, Massachusetts, pp 135–147. https://doi.org/10.1016/B978-0-12-801309-0.00006-9
Ji K, Wang Y, Sun W, Lou Q, Mei H, Shen S, Chen H (2012) Drought-responsive mechanisms in rice genotypes with contrasting drought tolerance during reproductive stage. J Plant Physiol 169(4):336–344. https://doi.org/10.1016/j.jplph.2011.10.010
Jovanović SV, Kukavica B, Vidović M, Morina F, Menckhoff L (2018) Class III peroxidases: functions, localization and redox regulation of isoenzymes. In: Gupta DK, Palma JM, Corpas FJ (eds) Antioxidants and Antioxidant Enzymes in Higher Plants. Springer, Cham, pp 269–300. https://doi.org/10.1007/978-3-319-75088-0_13
Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57(2):315–319. https://doi.org/10.1104/pp.57.2.315
Kavas M, Baloğlu MC, Akca O, Köse FS, Gökçay D (2013) Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turk J Biol 37(4):491–498. https://doi.org/10.3906/biy-1210-55
Khanna-Chopra R, Semwal VK, Lakra N, Pareek A (2019) Proline–a key regulator conferring plant tolerance to salinity and drought. In: Hasanuzzaman M, Fujita M, Oku H, Islam MT (eds) Plant Tolerance to Environmental Stress Role of Phytoprotectants. CRC Press, Boca Raton, pp 59–80. https://doi.org/10.1201/9780203705315
Kim YH, Yoo YJ (1996) Peroxidase production from carrot hairy root cell culture. Enzyme Microb Technol 18(7):531–535. https://doi.org/10.1016/0141-0229(95)00168-9
Kreuzwieser J, Meischner M, Grün M, Yáñez-Serrano AM, Fasbender L, Werner C (2021) Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles. New Phytol 232(5):1930–1943. https://doi.org/10.1111/nph.17736
Kuk YI, Shin JS, Burgos NR, Hwang TE, Han O, Cho BH, Jung S, Guh JO (2003) Antioxidative enzymes offer protection from chilling damage in rice plants. Crop Sci 43(6):2109–2117. https://doi.org/10.2135/cropsci2003.2109
Li D, Li C, Sun H, Wang W, Liu L, Zhang Y (2010) Effects of drought on soluble protein content and protective enzyme system in cotton leaves. Front Agric China 4(1):56–62. https://doi.org/10.1007/s11703-010-0102-2
Lin Y-C, Thùy TD, Wang S-Y, Huang P-L (2014) Type 1 diabetes, cardiovascular complications and sesame (芝麻 Zhī Má). J Tradit Complement Med 4(1):36–41. https://doi.org/10.4103/2225-4110.124817
Liu X-F, Sun W-M, Li Z-Q, Bai R-X, Li J-X, Shi Z-H, Geng H, Zheng Y, Zhang J, Zhang G-F (2013) Over-expression of ScMnSOD, a SOD gene derived from Jojoba, improve drought tolerance in Arabidopsis. J Integr Agric 12(10):1722–1730. https://doi.org/10.1016/S2095-3119(13)60404-9
Lu X, Xue X, Zhou X (2018) Response of growth and other physiological characteristics of Sophora Japonica L. saplings to drought stress. IOP Conf Ser: Earth Environ Sci 170(5):052029. https://doi.org/10.1088/1755-1315/170/5/052029
Lutts S, Kinet J, Bouharmont J (1995) Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. J Exp Bot 46(12):1843–1852. https://doi.org/10.1093/jxb/46.12.1843
Maheswari M, Varalaxmi Y, Sarkar B, Ravikumar N, Vanaja M, Yadav SK, Jyothilakshmi N, Vijayalakshmi T, Savita S, Rao MS (2021) Tolerance mechanisms in maize identified through phenotyping and transcriptome analysis in response to water deficit stress. Physiol Mol Biol Plants 27:1377–1394. https://doi.org/10.1007/s12298-021-01003-4
Mattos L, Moretti C (2015) Oxidative stress in plants under drought conditions and the role of different enzymes. Enzym Eng 5(1):1–6. https://doi.org/10.4172/2329-6674.1000136
McCready R, Guggolz J, Silviera V, Owens H (1950) Determination of starch and amylose in vegetables. Anal Chem 22(9):1156–1158. https://doi.org/10.1021/ac60045a016
McKersie BD, Bowley SR, Harjanto E, Leprince O (1996) Water-deficit tolerance and field performance of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol 111(4):1177–1181. https://doi.org/10.1104/pp.111.4.1177
Millar AH, Knorpp C, Leaver CJ, Hill SA (1998) Plant mitochondrial pyruvate dehydrogenase complex: purification and identification of catalytic components in potato. Biochem J 334(3):571–576. https://doi.org/10.1042/bj3340571
Mo Y, Yang R, Liu L, Gu X, Yang X, Wang Y, Zhang X, Li H (2016) Growth, photosynthesis and adaptive responses of wild and domesticated watermelon genotypes to drought stress and subsequent re-watering. Plant Growth Regul 79(2):229–241. https://doi.org/10.1007/s10725-015-0128-9
Morris JB (2002) Food, industrial, nutraceutical, and pharmaceutical uses of sesame genetic resources. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, pp 153–156
Moural TW, Lewis KM, Barnaba C, Zhu F, Palmer NA, Sarath G, Scully ED, Jones JP, Sattler SE, Kang C (2017) Characterization of class III peroxidases from switchgrass. Plant Physiol 173(1):417–433. https://doi.org/10.1104/pp.16.01426
Ndoumou OD, Ndzomo TG, Djocgoue PF (1996) Changes in carbohydrate, amino acid and phenol contents in cocoa pods from three clones after infection with Phytophthora megakarya Bra. and Grif. Ann Bot 77(2):153–158. https://doi.org/10.1006/anbo.1996.0017
Pandey BB, Ratnakumar P, Usha Kiran B, Dudhe MY, Lakshmi GS, Ramesh K, Guhey A (2021) Identifying traits associated with terminal drought tolerance in sesame (Sesamum indicum L.) genotypes. Front Plant Sci 12:739896. https://doi.org/10.3389/fpls.2021.739896
Poonam, Bhardwaj R, Handa N, Kaur H, Rattan A, Bali S, Gautam V, Sharma A, Ohri P, Thukral AK (2016) Sugar signalling in plants: a novel mechanism for drought stress management. In: Ahmad P (eds) Water Stress and Crop Plants: A Sustainable Approach. Wiley-Blackwell, Chichester, pp 287–302. https://doi.org/10.1002/9781119054450.ch19
Rai V (2002) Role of amino acids in plant responses to stresses. Biol Plant 45(4):481–487. https://doi.org/10.1023/A:1022308229759
Del Río LA, Corpas FJ, López-Huertas E, Palma JM (2018) Plant Superoxide dismutases: function under abiotic stress conditions. In: Gupta DK, Palma JM, Corpas FJ (eds) Antioxidants and Antioxidant Enzymes in Higher Plants. Springer, Cham, pp 1–26. https://doi.org/10.1007/978-3-319-75088-0_1
Ritchie SW, Nguyen HT, Holaday AS (1990) Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci 30(1):105–111. https://doi.org/10.2135/cropsci1990.0011183X003000010025x
Schmidt SB, Husted S (2019) The biochemical properties of manganese in plants. Plants 8(10):381. https://doi.org/10.3390/plants8100381
Sequera-Mutiozabal M, Antoniou C, Tiburcio AF, Alcázar R, Fotopoulos V (2017) Polyamines: emerging hubs promoting drought and salt stress tolerance in plants. Curr Mol Biol Rep 3(1):28–36. https://doi.org/10.1007/s40610-017-0052-z
Shah TM, Imran M, Atta BM, Ashraf MY, Hameed A, Waqar I, Shafiq M, Hussain K, Naveed M, Aslam M (2020) Selection and screening of drought tolerant high yielding chickpea genotypes based on physio-biochemical indices and multi-environmental yield trials. BMC Plant Biol 20:1–16. https://doi.org/10.1186/s12870-020-02381-9
Silva EN, Silveira JA, Aragão RM, Vieira CF, Carvalho FE (2019) Photosynthesis impairment and oxidative stress in Jatropha curcas exposed to drought are partially dependent on decreased catalase activity. Acta Physiol Plant 41(1):4. https://doi.org/10.1007/s11738-018-2794-5
Da Silva EC, De Albuquerque MB, De Azevedo Neto AD, Da Silva Junior CD (2013) Drought and its consequences to plants–from individual to ecosystem. In: Akinci S (ed) Responses of organisms to water stress. IntechOpen, Rijeka, pp 17–47. https://doi.org/10.5772/53833
Silveira R, Abreu F, Mamidi S, McClean P, Vianello R, Lanna A, Carneiro N, Brondani C (2015) Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa). Genet Mol Res 14(3):8181–8200. https://doi.org/10.4238/2015.July.27.6
Song J, Zeng L, Chen R, Wang Y, Zhou Y (2018) In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula. 3 Biotech 8:348. https://doi.org/10.1007/s13205-018-1373-1
Su X, Wei F, Huo Y, Xia Z (2017) Comparative physiological and molecular analyses of two contrasting flue-cured tobacco genotypes under progressive drought stress. Front Plant Sci 8:827. https://doi.org/10.3389/fpls.2017.00827
Taiz L, Zeiger E, Møller IM, Murphy A (2018) Fundamentals of plant physiology. Sinauer Associates, Sunderland
Thalmann M, Santelia D (2017) Starch as a determinant of plant fitness under abiotic stress. New Phytol 214(3):943–951. https://doi.org/10.1111/nph.14491
Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, Rozen SG (2012) Primer3—new capabilities and interfaces. Nucleic Acids Res 40(15):e115. https://doi.org/10.1093/nar/gks596
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3(7):1–12. https://doi.org/10.1186/gb-2002-3-7-research0034
Verma G, Srivastava D, Tiwari P, Chakrabarty D (2019) ROS modulation in crop plants under drought stress. In: Hasanuzzaman M, Fotopoulos V, Nahar K, Fujita M (eds) Reactive Oxygen, Nitrogen and Sulfur Species in Plants: Production, Metabolism, Signaling and Defense Mechanisms. Wiley-Blackwell, Chichester, pp 311–336. https://doi.org/10.1002/9781119468677.ch13
Wang W, Zhang X, Deng F, Yuan R, Shen F (2017) Genome-wide characterization and expression analyses of superoxide dismutase (SOD) genes in Gossypium hirsutum. BMC Genom 18(1):1–25. https://doi.org/10.1186/s12864-017-3768-5
Wang X, Wang M, Yan G, Yang H, Wei G, Shen T, Wan Z, Zheng W, Fang S, Wu Z (2023) Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis Front. Plant Sci 14:1117507. https://doi.org/10.3389/fpls.2023.1117507
Wu C, Ding X, Ding Z, Tie W, Yan Y, Wang Y, Yang H, Hu W (2019) The class III peroxidase (POD) gene family in cassava: identification, phylogeny, duplication, and expression. Int J Mol Sci 20(11):2730. https://doi.org/10.3390/ijms20112730
Yadav C, Bahuguna RN, Dhankher OP, Singla-Pareek SL, Pareek A (2022) Physiological and molecular signatures reveal differential response of rice genotypes to drought and drought combination with heat and salinity stress. Physiol Mol Biol Plants 28(4):899–910. https://doi.org/10.1007/s12298-021-00934-2
Yang Y, Li C (2016) Photosynthesis and growth adaptation of Pterocarya stenoptera and Pinus elliottii seedlings to submergence and drought. Photosynthetica 54(1):120–129. https://doi.org/10.1007/s11099-015-0171-9
Yemm E, Cocking E, Ricketts R (1955) The determination of amino-acids with ninhydrin. Anlst 80(948):209–209. https://doi.org/10.1039/AN9558000209
Yokota T, Matsuzaki Y, Koyama M, Hitomi T, Kawanaka M, Enoki-Konishi M, Okuyama Y, Takayasu J, Nishino H, Nishikawa A (2007) Sesamin, a lignan of sesame, down-regulates cyclin D1 protein expression in human tumor cells. Cancer Sci 98(9):1447–1453. https://doi.org/10.1111/j.1349-7006.2007.00560.x
Zanella M, Borghi GL, Pirone C, Thalmann M, Pazmino D, Costa A, Santelia D, Trost P, Sparla F (2016) β-amylase 1 (BAM1) degrades transitory starch to sustain proline biosynthesis during drought stress. J Exp Bot 67(6):1819–1826. https://doi.org/10.1093/jxb/erv572
Zegaoui Z, Planchais S, Cabassa C, Djebbar R, Belbachir OA, Carol P (2017) Variation in relative water content, proline accumulation and stress gene expression in two cowpea landraces under drought. J Plant Physiol 218:26–34. https://doi.org/10.1016/j.jplph.2017.07.009
Zhou S, Duursma RA, Medlyn BE, Kelly JW, Prentice IC (2013) How should we model plant responses to drought? An analysis of stomatal and non-stomatal responses to water stress. Agric Meteorol 182:204–214. https://doi.org/10.1016/j.agrformet.2013.05.009
Zhou L, Wang S, Chi Y, Li Q, Huang K, Yu Q (2015) Responses of photosynthetic parameters to drought in subtropical forest ecosystem of China. Sci Rep 5:18254. https://doi.org/10.1038/srep18254
Zhu Y, Luo X, Nawaz G, Yin J, Yang J (2020) Physiological and biochemical responses of four cassava cultivars to drought stress. Sci Rep 10(1):6968. https://doi.org/10.1038/s41598-020-63809-8
Acknowledgements
This study was supported by Sari Agricultural Sciences and Natural Resources University (SANRU) and Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT). The authors also wish to thank the laboratory staff of GABIT for their technical assistance.
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MAB, SKK, AD, and PM discussed the research concept and designed the experiment. MAB performed the experiment, analyzed the data, and wrote the manuscript. SKK, AD, and PM reviewed and edited the manuscript. All authors read and approved the final manuscript.
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Baghery, M.A., Kazemitabar, S.K., Dehestani, A. et al. Sesame (Sesamum indicum L.) response to drought stress: susceptible and tolerant genotypes exhibit different physiological, biochemical, and molecular response patterns. Physiol Mol Biol Plants 29, 1353–1369 (2023). https://doi.org/10.1007/s12298-023-01372-y
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DOI: https://doi.org/10.1007/s12298-023-01372-y