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Redox metabolic and molecular parameters for screening drought tolerant indigenous aromatic rice cultivars

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Abstract

The present work makes an effort to assess and standardize some redox metabolic and molecular parameters for screening drought tolerant indigenous aromatic rice cultivars of West Bengal, India. PEG-induced dehydration stress during early germination caused disruption of redox-homeostasis and oxidative damage in four IARVs (Jamainadu, Tulaipanji, Sitabhog and Badshabhog) by enhancing the accumulation of pro-oxidants [assessed in terms of oxidation of 2′,7′-dichlorofluorescindiacetate (DCFDA), accumulation of \({\text{O}}_{2}^{ \cdot - }\) and H2O2 and in situ staining of reactive oxygen species (ROS) in germinating tissue], significant reduction of antioxidative defence (total antioxidant and radical scavenging capacity, total thiol content and activities of antioxidative defence enzymes) and aggravating protein oxidation and lipid peroxidation (assessed in terms of free carbonyl content and accumulation of thiobarbituric acid reactive substances). When compared between the indigenous aromatic rice cultivars, a clear trend in differential redox regulatory properties in which ROS-antioxidant interaction acts at metabolic interface for redox homeostasis was observed in the order Badshabhog > Tulaipanji > Sitabhog > Jamainadu. Moreover, when the efficacy of ascorbate–glutathione cycle for scavenging H2O2 generated during dehydration stress was assessed and compared between the landraces exposed to PEG-induced dehydration stress in germinating tissue, it also exhibited almost the same trend with the landrace Tulaipanji and Badsabhog exhibiting maximum and Jamainadu the minimum efficiencies of the redox cycle. The indigenous aromatic rice cultivars Tulaipanji and Badsabhog resist dehydration stress better than the other two landraces due to its early preparedness to combat oxidative stress by up-regulating expression of genes of some enzymes of ascorbate–glutathione cycle along with some other antioxidative enzymes. A model of redox homeostasis in which ROS-antioxidant (ascorbate–glutathione system) acts at metabolic interface for up-regulation of antioxidative gene expression necessary for differential drought stress tolerance among the indigenous aromatic rice varieties is suggested.

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Acknowledgements

ND acknowledges The University of Burdwan, West Bengal, for State Funded Research Fellowship (136/35, 31.07.31.07.2014, Government of West Bengal, India). Assistance from Anirban Bhar, Faculty Ramakrishna Mission Vivekananda Centenary College, Kolkata, for Semi-quantitative RT-PCR is thankfully acknowledged. Instrumentation facility of CAS (UGC), Govt. of India to the Department of Botany, University of Burdwan is also gratefully acknowledged.

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  1. Centre for Advanced Study, Department of Botany, The University of Burdwan, Burdwan, West Bengal, India

    Soumen Bhattacharjee & Nivedita Dey

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  1. Soumen Bhattacharjee
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  2. Nivedita Dey
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Correspondence to Soumen Bhattacharjee.

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Fig. 5

Effect of different magnitude of PEG induced imbibitional dehydration stress (− 0.344, − 0.851 and − 1.619 MPa) on visualization of superoxide (A) and hydrogen peroxide (B) of four indigenous aromatic rice varieties of Oryza sativa L. (Tulaipanji, Sitabhog, Badshabhog and Jamainadu) (DOC 601 kb)

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Bhattacharjee, S., Dey, N. Redox metabolic and molecular parameters for screening drought tolerant indigenous aromatic rice cultivars. Physiol Mol Biol Plants 24, 7–23 (2018). https://doi.org/10.1007/s12298-017-0484-1

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