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Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing olymorphisms between isolates of this species.

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Authors and Affiliations

  1. Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, México

    Daisy Pérez-Brito, Anuar Magaña-Alvarez, Alberto Cortes-Velazquez, Claudia Torres-Calzada & Raul Tapia-Tussell

  2. Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, México DF., 04510, México

    Patricia Lappe-Oliveras & Teófilo Herrera-Suarez

  3. Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, México

    Alfonso Larqué-Saavedra

Authors
  1. Daisy Pérez-Brito
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  2. Anuar Magaña-Alvarez
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  3. Patricia Lappe-Oliveras
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  4. Alberto Cortes-Velazquez
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  5. Claudia Torres-Calzada
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  6. Teófilo Herrera-Suarez
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  7. Alfonso Larqué-Saavedra
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  8. Raul Tapia-Tussell
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Correspondence to Raul Tapia-Tussell.

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Pérez-Brito, D., Magaña-Alvarez, A., Lappe-Oliveras, P. et al. Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting. J Microbiol. 53, 14–20 (2015). https://doi.org/10.1007/s12275-015-4373-4

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  • DOI: https://doi.org/10.1007/s12275-015-4373-4

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