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Enhancing the peroxidase-like activity of MoS2-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione

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Abstract

Nanozymes are next-generation of nanomaterials with enzyme-like activities. In particular, nanozymes with peroxidase (POD)-like activity have been utilized in various fields, including antibacterial, detection, degradation, etc. However, their extensive applications were limited by their low catalytic activity currently. Herein, we have presented a composite nanozyme based on attapulgite (ATP) (Fe-ATP-MoS2 (FAM)), which exhibited enhanced POD-like activity (185.33 U·mg−1), 4.25 times higher than that of Fe-MoS2 (FM) (43.63 U·mg−1). The density functional theory (DFT) calculations indicated that the addition of ATP increased the electron density of metal centers (Mo and Fe). More importantly, Michaelis–Menten kinetics revealed that the introduction of ATP significantly enhanced the binding affinities of substrates through the pores of ATP, forming a highly concentrated substrate microenvironment and thus promoting its POD-like activity. Additionally, from molecular size and kinetic analysis, we proposed that the changes in substrate size before and after oxidation also significantly affected its Michaelis-constant (Km) value. Furthermore, we utilized FAM in the applications of highly effective antibacterial application and sensitive detection of glutathione (GSH). In conclusion, this work provides a novel approach for designing a highly efficient nanozyme based on natural mineral composites.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 52072347 and 22272152), the Foundation of Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (No. CMAR-2022-04), and Beijing Nova project (No. 20220484155).

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Authors and Affiliations

  1. Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing, 100083, China

    Feng Feng, Yihe Zhang, Xiao Zhang, Jiahe Zhang, Wenjie Qu, Wangshu Tong, Qi An & Lu Zhao

  2. Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Bin Mu

  3. Experimental Center of Advanced Materials School of Materials Science and Engineering, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China

    Minmin Liang & Zhanjun Guo

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  1. Feng Feng
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  2. Yihe Zhang
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  3. Xiao Zhang
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  4. Bin Mu
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  5. Jiahe Zhang
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  9. Qi An
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  10. Zhanjun Guo
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  11. Lu Zhao
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Correspondence to Yihe Zhang, Zhanjun Guo or Lu Zhao.

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Enhancing the peroxidase-like activity of MoS2-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione

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Feng, F., Zhang, Y., Zhang, X. et al. Enhancing the peroxidase-like activity of MoS2-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione. Nano Res. (2024). https://doi.org/10.1007/s12274-024-6685-3

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