Nano Research

, Volume 2, Issue 7, pp 543–552 | Cite as

Direct imaging of titania nanotubes located in mouse neural stem cell nuclei

Open Access
Research Article


Titania nanotubes (TiO2-NTs) are a potential drug vehicle for use in nanomedicine. To this end, a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out. TiO2-NTs were first conjugated with a fluorescent label, fluorescein isothiocyanate (FITC). FITC-conjugated titania nanotubes (FITC-TiO2-NTs) internalized in mouse neural stem cells (NSCs, line C17.2) can be directly imaged by confocal microscopy. The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells. After co-incubation with cells for 24 h, FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca. More interestingly, the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) were also employed in tracking the nanotubes in the cell. These results will be of benefit in future studies of TiO2-NTs for use as a drug vehicle, particularly for DNA-targeting drugs.


Titania nanotubes mouse neural stem cells nucleus confocal imaging atomic force microscopy 


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Copyright information

© Tsinghua University Press and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Yanli Wang
    • 1
  • Jia Wang
    • 1
  • Xiaoyong Deng
    • 1
  • Jiao Wang
    • 2
  • Haifang Wang
    • 1
  • Minghong Wu
    • 1
  • Zheng Jiao
    • 1
  • Yuanfang Liu
    • 1
    • 3
  1. 1.Institute of Nanochemistry and NanobiologyShanghai UniversityShanghaiChina
  2. 2.Institute of Systems BiologyShanghai UniversityShanghaiChina
  3. 3.Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina

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