Skip to main content

Advertisement

SpringerLink
Log in

Funktionale Zelllinien durch neue Immortalisierungsprotokolle

Zellsysteme

  • Wissenschaft · Special: Zellbiologi
  • Published:
BIOspektrum Aims and scope

Abstract

Cell lines are immortal cultures of cells that are used in research as well as for production of pharmaceuticals. Currently available cell lines are derived from normal and tumorous tissue of humans and diverse animal species, however, with considerable loss of specific properties. The development of new immortalization protocols has led to a significant improvement of the properties of the resulting cell lines. These retain specific properties and activities that include functional grafting.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Bodnar AG, Ouellette M, Frolkis M et al. (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279:349–352

    Article  PubMed  CAS  Google Scholar 

  2. Simonsen JL, Rosada C, Serakinci N et al. (2002) Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol 20:592–596

    Article  PubMed  CAS  Google Scholar 

  3. Yang J, Nagavarapu U, Relloma K et al. (2001) Telomerized human microvasculature is functional in vivo. Nat Biotechnol 19:219–224

    Article  PubMed  CAS  Google Scholar 

  4. May T, Butueva M, Bantner S et al. (2010) Synthetic gene regulation circuits for control of cell expansion. Tissue Eng Part A 16:441–452

    Article  PubMed  CAS  Google Scholar 

  5. Noguchi H, Kobayashi N, Westerman KA et al. (2002) Controlled expansion of human endothelial cell populations by Cre-loxP-based reversible immortalization. Hum Gene Ther 13:321–334

    Article  PubMed  CAS  Google Scholar 

  6. Rybkin II, Markham DW, Yan Z et al. (2003) Conditional expression of SV40 T-antigen in mouse cardiomyocytes facilitates an inducible switch from proliferation to differentiation. J Biol Chem 278:15927–15934

    Article  PubMed  CAS  Google Scholar 

  7. Westerman KA, Leboulch P (1996) Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc Natl Acad Sci USA 93:8971–8976

    Article  PubMed  CAS  Google Scholar 

  8. Botezatu L, Sievers S, Gama-Norton L et al. (2011) Genetic aspects of cell line development from a synthetic biology perspective. Adv Biochem Eng Biotechnol 127:251–284

    Google Scholar 

  9. May T, Eccleston L, Herrmann S et al. (2008) Bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit. PLoS ONE 3:e2372

    Article  PubMed  Google Scholar 

  10. May T, Hauser H, Wirth D (2004) Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization. Nucleic Acids Res 32:5529–5538

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Inscreenex GMBH, Braunschweig, Germany

    Tobias May

  2. Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany

    Hansjörg Hauser & Dagmar Wirth

  3. Helmholtz-Zentrum für Infektionsforschung — HZI, Abt. Genregulation und Differenzierung, Inhoffenstraße 7, D-38124, Braunschweig, Germany

    Hansjörg Hauser

Authors
  1. Tobias May
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hansjörg Hauser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dagmar Wirth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hansjörg Hauser.

Additional information

Tobias May 1996–2001 Biochemiestudium, Universität Halle-Wittenberg. 2005 Promotion in der Gruppe von Dr. D. Wirth am Helmholtz-Zentrum für Infektionsforschung. 2005–2011 Postdoc in der Abteilung Genregulation und Differenzierung am Helmholtz-Zentrum für Infektionsforschung. Seit 2011 Mitgeschäftsführer InSCREENeX GmbH, Braunschweig.

Hansjörg Hauser 1968–1973 Studium der Lebensmitteltechnologie und Ernährungswissenschaft an der Universität Hohenheim. 1977 Promotion an der Universität Konstanz in der Gruppe von Prof. Dr. R. Knippers. 1978–1980 Postdoc mit Prof. Dr. G. Schütz am MPI für Molekulare Genetik, Berlin, und am DKFZ, Heidelberg. 1981–1985 wissenschaftlicher Angestellter an der Gesellschaft für Biotechnologische Forschung (GBF), Braunschweig. 1986–1994 Leiter der AG Genetik von Eukaryonten. Seit 1994 Leiter der Abteilung Genregulation und Differenzierung, Helmholtz-Zentrum für Infektionsforschung, Braunschweig.

Dagmar Wirth 1982–1987 Chemiestudium an der TU Braunschweig. 1991 Promotion in der Gruppe von Prof. Dr. J. Bode. 1991–1996 Postdoc in der AG Hauser der GBF in Braunschweig. 1996–1998 freiberuflich tätig in der biomedizinischen Forschung. 1999–2003 wissenschaftliche Mitarbeiterin der Medizinischen Hochschule Hannover. Seit 2004 wissenschaftliche Mitarbeiterin am Helmholtz-Zentrum für Infektionsforschung; dort seit 2007 Leiterin der AG Modellsysteme für Infektion und Immunität.

Rights and permissions

Reprints and permissions

About this article

Cite this article

May, T., Hauser, H. & Wirth, D. Funktionale Zelllinien durch neue Immortalisierungsprotokolle. Biospektrum 18, 390–393 (2012). https://doi.org/10.1007/s12268-012-0199-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-012-0199-3

Search

Navigation