Abstract
When we use cell cultures in research, we rarely think about the story behind them, which can be intriguing, providing insight, and sometimes tragic. In the 1950s, the culture of HeLa cells unexpectedly became well known scientifically and became one of the most famous cell cultures. These cells were taken from a woman named Henrietta Lacks, who had cervical cancer and died shortly afterward, and the HeLa cell line proved to be an essential tool for several generations of scientists around the world in developing new treatments and biomedical research. These cells have become unique due to their immortality, endless division, easy cultivation, and adaptation to conservation conditions. At the same time, HeLa cells remain a simplified imitation of the human body.
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REFERENCES
Hayflick, L. and Moorhead, P.S., The serial cultivation of human diploid cell strains, Exp. Cell Res., 1961, vol. 25, pp. 585–621.
Greider, C.W., Telomerase discovery: the excitement of putting together pieces of the puzzle (Nobel lecture), Angew. Chem. Int. Ed. Engl., 2010, vol. 49, no. 41, pp. 7422–7439. https://doi.org/10.1002/anie.201002408
Wang, L.J., Ma, F., Tang, B., and Zhang, C.Y., Sensing telomerase: From in vitro detection to in vivo imaging, Chem. Sci., 2017, vol. 8, no. 4, pp. 2495–2502. https://doi.org/10.1039/C6SC04801C
Lucey, B.P., Nelson-Rees, W.A., and Hutchins, G.M., Henrietta Lacks, HeLa cells, and cell culture contamination, Arch. Pathol. Lab. Med., 2009, vol. 133, no. 9, pp. 1463–1467. https://doi.org/10,1043/1543-2165-133.9.1463.
Gilgenkrantz, S., Requiem for Henrietta, Med. Sci. (Paris), 2010, vol. 5, pp. 529–533. https://doi.org/10.1051/medsci/2010265529
Heng, H.H., HeLa genome versus donor’s genome, Nature, 2013, vol. 501, p. 167. https://doi.org/10.1038/501167d
Kochetkov, D.V., Ilyinskaya, G.V., Komarov, P.G., et al., Transcriptional inhibition of the human papilloma virus reactivates tumor suppressor p53 in cervical carcinoma cells, Mol. Biol., 2007, vol. 41, no. 3, pp. 459–466.
Wu, L. and Qu, X.G., Cancer biomarker detection: recent achievements and challenges, Chem. Soc. Rev., 2015, vol. 44, pp. 2963–2997.
Sfeir, A. and Lange, T.D., Removal of shelterin reveals the telomere end-protection problem, Science, 2012, vol. 336, pp. 593–597.
Scherer, W.F., Syverton, J.T., and Gey, G.O., Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix, J. Exp. Med., 1953, vol. 97, pp. 695–710. https://doi.org/10.1084/jem.97.5.695
Berg, P. and Mertz, J.E., Personal reflections on the origins and emergence of recombinant DNA technology, Genetics, 2010, vol. 184, no. 1, pp. 9–17. https://doi.org/10.1534/genetics.109.112144
Landry, J.J., Pyl, P.T., Rausch, T., et al., The genomic and transcriptomic landscape of a HeLa cell line, G3: Genes, Genomes,Genet., 2013, vol. 3, no. 8, pp. 1213–1224. https://doi.org/10.1534/g3.113.005777
Svalastog, A.L. and Martinelli, L., Representing life as opposed to being: the bio-objectification process of the HeLa cells and its relation to personalized medicine, Croat. Med. J., 2013, vol. 54, pp. 397–402. https://doi.org/10.3325/cmj.2013.54.397
Frattini, A., Fabbri, M., Valli, R., De Paoli, E., Montalbano, G., Gribaldo, L., Pasquali, F., and Maserati, E., High variability of genomic instability and gene expression profiling in different HeLa clones, Sci. Rep., 2015, vol. 5, p. 15377. https://doi.org/10.1038/srep15377
Callaway, E., Deal done over HeLa cell line, Nature, 2013, vol. 500, pp. 132–133. https://doi.org/10.1038/500132a
Hudson, K.L. and Collins, F.S., Biospecimen policy: Family matters, Nature, 2013, vol. 500, pp. 141–142. https://doi.org/10.1038/500141a
Knauss, S. and Klein, A., From aneuploidy to cancer: The evolution of a new species?, J. Biosci., 2012, vol. 37, p. 211. https://doi.org/10.1007/s12038-012-9199-1
Van Valen, L.M. and Maiorana, V.C., HeLa, a new microbial species, Evol. Theory Rev., 1991, vol. 10, pp. 71–74.
Strathmann, R.R., From metazoan to protist via competition among cell lineages, Evol. Theory, 1991, vol. 10, pp. 67–70.
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Translated by A. Panyushkina
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Lyapun, I.N., Andryukov, B.G. & Bynina, M.P. HeLa Cell Culture: Immortal Heritage of Henrietta Lacks. Mol. Genet. Microbiol. Virol. 34, 195–200 (2019). https://doi.org/10.3103/S0891416819040050
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DOI: https://doi.org/10.3103/S0891416819040050