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Picrasma quassioides inhibits LPS- and IFN-γ-stimulated nitric oxide production and inflammatory response in RAW264.7 macrophage cells

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Abstract

The present study was designed to investigate the anti-inflammatory effects of Picrasma quassioides (P. quassioides) in lipopolysaccharide (LPS)- and interferon (IFN)-γ-stimulated RAW 264.7 cells. P. quassioides has been used as a traditional medicine for the treatment of gastro-enteritis, eczema, and snakebite. P. quassioides significantly decreased LPS- and IFN-γ-stimulated nitric oxide (NO) production in a concentration-dependent manner. Real-time PCR or Western blotting confirmed that the expression of the extra-cellular signal-regulated kinase (ERK), p42 and, p38 mitogen-activated protein kinases (MAPKs), c-Jun N-terminal kinase, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) mediated MAPK signaling pathways in LPS- and IFN-γ-stimulated RAW264.7 cells. Mechanistic studies revealed the activities of nuclear factor kappa B (NF-κB). As P. quassioides regulated the gene expression of iNOS and COX-2 in RAW264.7 cells, it might be a promising agent for the prevention and/or treatment of various inflammatory diseases.

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Authors and Affiliations

  1. Department of Dermatology, Chung-Ang University College of Medicine, Seoul, 156-756, Korea

    Yi Seop Song, Yonghee Lee, Tae-Rin Kwon & Beom Joon Kim

  2. Department of Medicine, The Graduated school, Chung-Ang University College of Medicine, Seoul, 156-756, Korea

    Yi Seop Song, Tae-Rin Kwon & Beom Joon Kim

  3. R&D Center, Bioland, Cheonan, 330-863, Korea

    Young Heui Kim

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  1. Yi Seop Song
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Song, Y.S., Lee, Y., Kwon, TR. et al. Picrasma quassioides inhibits LPS- and IFN-γ-stimulated nitric oxide production and inflammatory response in RAW264.7 macrophage cells. Biotechnol Bioproc E 19, 404–410 (2014). https://doi.org/10.1007/s12257-014-0131-4

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  • DOI: https://doi.org/10.1007/s12257-014-0131-4

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