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Alpinia katsumadai HAYATA Seed Extract Inhibit LPS-Induced Inflammation by Induction of Heme Oxygenase-1 in RAW264.7 Cells

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Abstract

In the present study, we investigated the effects of Alpinia katsumadai HAYATA (Zingiberaceae) seed ethanolic extract (AKEE) and its three components on the production of inflammatory mediators and some potential underlying mechanisms in lipopolysaccharide (LPS)-induced inflammation RAW264.7 cells. The whole formula, AKEE, and three major component compounds were then evaluated for their effects on inflammation-related parameters using LPS-induced RAW264.7 cells. Production of namely nitric oxide (NO) and cytokine levels were measured by the Griess reagent and ELISA, respectively. To investigate the underlying mechanisms of anti-inflammatory activities of AKEE, protein expression of nitric oxide synthase (inducible nitric oxide synthase, iNOS), heme oxygenase-1 (HO-1), and nuclear factor-kappa B (NF-κB) were evaluated by western blot analysis. AKEE and the major group of compounds in AKEE (alpinetin, cardamonin, and pinocembrin) complement exert anti-inflammatory effects for NO and PGE2 production. In addition, AKEE treatment significantly inhibited the LPS-induced production of interleukin-6 and tumor necrosis factor (TNF)-α, as well as the expression of iNOS. AKEE also induced HO-1 expression in RAW264.7 cells and inhibited the nuclear translocation of NF-κB by preventing degradation of the inhibitor kappa B-alpha. We also demonstrated that the effects of AKEE on TNF-α production were partially reversed by the HO-1 inhibitor tin protoporphyrin. These results indicate that AKEE and its major component may have anti-inflammatory activity via induction of HO-1 expression was partly responsible for the anti-inflammatory effects.

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ACKNOWLEDGMENTS

This research was supported by a grant “Evidence-based Medicine for herbal formulae” from Korea Institute of Oriental Medicine (KIOM).

Competing Interests

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Exporo 483, Daejeon, 305-811, Republic of Korea

    Mee-Young Lee, Chang-Seob Seo, Jin-Ah Lee, In-Sik Shin, Su-Jeong Kim, HeyKyung Ha & Hyeun-Kyoo Shin

  2. College of Pharmacy Division of Life and Pharmaceutical Sciences Ewha Womans University, Science bldg C206, 11-1 Daehyun-dong, Sudaemun-gu, Seoul, 120-750, South Korea

    Jin-Ah Lee

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  1. Mee-Young Lee
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  2. Chang-Seob Seo
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Correspondence to Hyeun-Kyoo Shin.

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Mee-Young Lee and Chang-Seob Seo equally contributed to this work.

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Lee, MY., Seo, CS., Lee, JA. et al. Alpinia katsumadai HAYATA Seed Extract Inhibit LPS-Induced Inflammation by Induction of Heme Oxygenase-1 in RAW264.7 Cells. Inflammation 35, 746–757 (2012). https://doi.org/10.1007/s10753-011-9370-0

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