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Bcl-2 Antisense Oligonucleotide Inhibits the Proliferation of Childhood Leukemia/lymphoma Cells of the B-cell Lineage

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Pathology & Oncology Research

Abstract

An 18-mer phosphorothioate bcl-2 atisense oligonucleotide (ASO) inhibited colony formation of three B-cell leukemia/lymphoma cell lines in a dose dependent manner in the range of 0.125–0.5 μmol/l. The srcambled cogener had no detectable effect. A decrease in BCL-2 protein and apoptotic DNA fragmentation was detected in the studied cell lines and primary blast cells of two children with acute lymphoblastic leukemia. Neither BCL-2 protein level, nor DNA integrity was affected by the scrambled control indicating the specific effect ASO. As far as we know, this is the first report on the effects of bcl-2 ASO on childhood leukemia/lymphoma cell samples.

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Acknowledgement

This work was supported in part by grant of the National Scientific Research Found (OTKA, No: T038307), “For the Leukemic Children” and “ Hope for the Leukemic Children” foundations.

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Authors and Affiliations

  1. Department of Pediatrics, Medical and Health Science Center, University of Debrecen, 98 Nagyerdei Circle, 4012, Debrecen, P.O. Box 32, Hungary

    István Szegedi, Anna Molnár & Csongor Kiss

  2. Department of Biochemistry and Molecular Biology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary

    Klára Katona, András Horváth & János Aradi

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  1. István Szegedi
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  2. Klára Katona
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  3. András Horváth
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  4. Anna Molnár
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  5. János Aradi
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  6. Csongor Kiss
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Correspondence to Csongor Kiss.

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Szegedi, I., Katona, K., Horváth, A. et al. Bcl-2 Antisense Oligonucleotide Inhibits the Proliferation of Childhood Leukemia/lymphoma Cells of the B-cell Lineage. Pathol. Oncol. Res. 14, 275–279 (2008). https://doi.org/10.1007/s12253-008-9076-2

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  • DOI: https://doi.org/10.1007/s12253-008-9076-2

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