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Potato Virus X (PVX) Elimination as Short and Long Term Effects of Hydrogen Peroxide and Salicylic Acid Is differentially Mediated by Oxidative Stress in Synergism with Thermotherapy

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Abstract

Short and long term effects of hydrogen peroxide (H2O2) and salicylic acid (SA) were examined in: thermotolerance, virus X (PVX)-free microplants obtained by in vitro thermotherapy, catalase activity (CAT) and H2O2 concentration. Short term effects (STE) were tested as follows: (a) nodal explants were cultivated 30 d on MS medium containing SA 10−5 or 10−6 M; and (b) nodal explants were waterlogged for 1 h in 1 or 5 mM H2O2 solution and subsequently cultured in MS for 30 d. Long term effects were tested as follows (LTE): the experimental regime was identical to STE but SA and H2O2 treated plants were subcultured for an additional 30 d period on MS. All treatments were followed by thermotherapy (32–42 C) for 35 d. Results showed SA and H2O2 induced thermotolerance during thermotherapy. The percentage of PVX-free plants obtained in H2O2 was significantly higher than in SA in STE and LTE by 3-and 4 fold respectively. CAT activity was differentially mediated by SA and H2O2.

Resumen

Se estudiaron los efectos del peróxido de hidrógeno (H2O2) y ácido salicílico (SA) a corto y largo plazo en: termotolerancia, microplantas libres de virus X (PVX) obtenidas por termoterapia in vitro, actividad catalasa (CAT) y contenido de H2O2. Efectos a corto plazo (ECP) se estudiaron como sigue: (a) explantes nodales fueron cultivados 30 d en medio MS con 10−5 o 10−6 M, y (b) explantes nodales fueron inundados 1 h en soluciones 1 mM o 5 mM de H2O2 y después se cultivaron en MS por 30 d. Efectos a largo plazo (ELP) se estudiaron como sigue: el protocolo experimental fue idéntico a ECP pero las plantas tratadas con SA y H2O2 fueron subcultivadas por un periodo adicional de 30 d en MS. Todos los tratamientos fueron seguidos por termoterapia (32–42 C) por 35 d. Los resultados mostraron que SA y H2O2 indujeron termotolerancia durante termoterapia. El porcentaje de plantas libres de PVX obtenido en H2O2 fue significativamente mayor que en SA en ECP y ELP en 3 y 4 veces respectivamente. La actividad CAT fue mediada diferencialmente por SA y H2O2.

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Acknowledgments

This research was supported by a grant from Recursos Fiscales, INIFAP.

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Authors and Affiliations

  1. Laboratorio de Fisiología-Biotecnología, Programa Nacional de Papa, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Conjunto SEDAGRO, Metepec, Estado de México, C.P. 52140, México

    Miguel Aguilar-Camacho & Humberto A. López-Delgado

  2. Centro Universitario Tenancingo Universidad Autónoma del Estado de México, Carretera Tenancingo-Villa Guerrero Km 1.5, Estado de México, C. P. 52400, México

    Martha E. Mora-Herrera

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  1. Miguel Aguilar-Camacho
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  2. Martha E. Mora-Herrera
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  3. Humberto A. López-Delgado
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Correspondence to Humberto A. López-Delgado.

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Aguilar-Camacho, M., Mora-Herrera, M.E. & López-Delgado, H.A. Potato Virus X (PVX) Elimination as Short and Long Term Effects of Hydrogen Peroxide and Salicylic Acid Is differentially Mediated by Oxidative Stress in Synergism with Thermotherapy. Am. J. Potato Res. 93, 360–367 (2016). https://doi.org/10.1007/s12230-016-9509-5

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