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Degrading ability of oligocyclic aromates by Phanerochaete sordida selected via screening of white rot fungi

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Abstract

Seventy-nine white rot strains were screened to determine if they had the potential for use in the degradation of oligocyclic aromates (PAHs) by measuring their dye-decoloration rate. Fourteen strains that were selected based on their dye-decoloration rate were then evaluated for the ability to tolerate various levels of PAHs spiked in agar medium. The ability of white rot fungi to degrade 3- or 4-ring PAHs (anthracene, phenanthrene, fluoranthene, pyrene) was determined. Two strains of Phanerochaete sordida (KUC8369, KUC8370) were possible PAHs degraders, degrading a significantly greater amount of phenanthrene and fluoranthene than the culture collection strain P. chrysosporium (a known PAHs degrader). The production of manganese peroxidase, the only extracellular ligninolytic enzyme detected during the cultivation, was evaluated.

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Abbreviations

ANT:

anthracene

PHE:

phenanthrene

ELM(s):

extracellular ligninolytic enzyme(s)

LiP:

lignin peroxidase

GC:

gas chromatography

MEA:

malt extract agar medium

MS:

mass spectrometry

FLT:

fluoranthene

PYR:

pyrene

LAC:

laccase (phenol oxidase)

MnP:

Mn-dependent peroxidase

PAH(s):

oligocyclic aromate(s) (‘polycyclic aromatic hydrocarbons’)

RBBR:

Remazol brilliant blue R

WRF:

white rot fungus(i)

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Authors and Affiliations

  1. Division of Environmental Science& Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 136-713, Seoul, Korea

    H. Lee, Y. -S. Choi, M. -J. Kim, N. -Y. Huh, G. -H. Kim & J. -J. Kim

  2. National Institute of Biological Resources (NIBR), Incheon, 404-708, Korea

    Y. W. Lim

  3. Department of Forest Resources Utilization, Korea Forest Research Institute, 130-712, Seoul, Korea

    S. -M. Kang & S. -T. Cho

  4. Division of Environmental Science & Ecological Engineering, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-713, Korea

    J. -J. Kim

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  1. H. Lee
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  4. N. -Y. Huh
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Correspondence to J. -J. Kim.

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Lee, H., Choi, Y.S., Kim, M.J. et al. Degrading ability of oligocyclic aromates by Phanerochaete sordida selected via screening of white rot fungi. Folia Microbiol 55, 447–453 (2010). https://doi.org/10.1007/s12223-010-0075-9

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  • DOI: https://doi.org/10.1007/s12223-010-0075-9

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