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Antibiotic resistance of Stenotrophomonas maltophilia strains isolated from captive snakes

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Abstract

The minimum inhibitory concentrations (MICs) of 24 antibiotics were determined for 45 Stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 °C (after 24 h and 48 h of incubation). The isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (based on PFGE) of the same origin were discarded. At 37 °C, the isolates showed a low frequency of resistance to levofloxacin (0 and 8.9 % of resistant strains after 24 and 48 h, MICs50 0.5 and 1 mg/L, MICs90 1 and 2 mg/L) and cotrimoxazole (2.2 % of resistant strains for 24 and 48 h, MICs50 4 mg/L for both time periods, MICs90 4 and 8). At 30 °C, the most effective drugs were also cotrimoxazole (2.2 and 6.7 %, MICs50 4 and 8, MICs90 8 and 32) and levofloxacin (8.9 and 46.7 %, MICs50 1 and 2, MICs90 2 and 4). The isolates were either identically or more susceptible to antibiotics than strains acquired from patients hospitalized at Olomouc University Hospital (the same region) with the exception of ciprofloxacin, cefoperazone, cefoperazone/sulbactam and ceftazidime.

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Abbreviations

AMK:

amikacin

AMP:

ampicillin

ATM:

aztreonam

CAZ:

ceftazidime

CFP:

cefoperazone

CFZ:

cefazolin

CHL:

chloramphenicol

CIP:

ciprofloxacin

COL:

colistin

CPS:

cefoperazone/sulbactam

CTX:

cefotaxime

FEP:

cefepime

FOX:

cefoxitin

GEN:

gentamicin

LVX:

levofloxacin

MEM:

meropenem

OFX:

ofloxacin

PIP:

piperacillin

CXM:

cefuroxime

SAM:

ampicillin/sulbactam

SXT:

cotrimoxazole

TET:

tetracycline

TOB:

tobramycin

TZP:

piperacillin/tazobactam

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, 775 15, Olomouc, Czech Republic

    P. Hejnar, M. Kolář & P. Sauer

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  1. P. Hejnar
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  2. M. Kolář
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  3. P. Sauer
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Correspondence to P. Hejnar.

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Hejnar, P., Kolář, M. & Sauer, P. Antibiotic resistance of Stenotrophomonas maltophilia strains isolated from captive snakes. Folia Microbiol 55, 83–87 (2010). https://doi.org/10.1007/s12223-010-0014-9

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  • DOI: https://doi.org/10.1007/s12223-010-0014-9

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