Abstract
Susceptibility to 20 antibiotics was tested in 104Stenotrophomonas maltophilia strains at 37 and 30°C by means of a dilution micromethod to verity the phenomenon of temperature-dependent susceptibility (TDS). Trimethoprim-sulfamethoxazole, pefloxacin and ofloxacin were the most active preparations at 37°C (93, 90, and 86% of susceptible strains, respectively), whilst trimethoprim-sulfamethoxazole, cefoperazone-sulbactam and pefloxacin performed best at 30°C (94, 94, and 76% of susceptible strains, respectively). Variants 37TDS (minimum inhibitory concentration, MIC, of tested antibiotics at least 4-times lower at 37 than at 30°C) occurred in 60%. Variants 30TDS (at least 4-times lower value of MIC at 30 than at 37°C) were found in 7.7%. Both variants in susceptibility to tested antibiotics appeared in 23.1%, whilst neither of them was observed in 9.6%. The 37TDS phenomenon was recorded most of all with gentamicin (51% of strains), amikacin (47), colistin (44) and tetracycline (34). The 30TDS phenomenon was found particularly with cefoperazone-sulbactam (16.0% of strains) and colistin (10.0%). The above phenomena may be due to changes in membrane permeability, temperature-dependent ribosomal changes, and insufficient adaptation to higher temperatures of some strains of the originally environmental speciesS. maltophilia.
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Hejnar, P., Kolář, M., Hájek, V. et al. Occurrence of variants with temperature-dependent susceptibility (TDS) to antibiotics amongStenotrophomonas maltophilia clinical strains. Folia Microbiol 46, 151–155 (2001). https://doi.org/10.1007/BF02873595
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DOI: https://doi.org/10.1007/BF02873595