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Effects of decreased specific glutathione reductase activity in a chromate-tolerant mutant of Schizosaccharomyces pombe

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Abstract

A chromate-tolerant mutant chr1-663T bearing a stable one-gene mutation and its parental strain 6chr+ were used to investigate the background of CrVI tolerance in the fission yeast Schizosaccharomyces pombe. The mutant chr1-663T displayed a significantly decreased specific glutathione reductase (GR) activity coded by the pgr1 + gene compared with its parental strain. Transformants of the mutant chr1-663T with a nonintegrative pUR18N vector expressing the pgr1 + gene exhibited the same CrVI sensitivity and specific GR activity as their parental strain, demonstrating the importance of the GR-NADPH system in CrVI tolerance. Transformants, nevertheless, exhibited an increased intracellular peroxide concentration, a decreased CrVI-reducing and HO•-producing ability, which suggested an unbalanced oxidoreduction state of cells and partial complementation of the GR function. No mutation was found in the sequences of the pgr1 + and the pap1 + (transcriptional regulatory gene of GR) genes of the CrVI-tolerant mutant by sequence analysis.

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Abbreviations

BPN:

N-tert-butyl-α-phenylnitrone

GR:

glutathione reductase

t-BuOOH:

tert-butylhydroperoxide

GSH:

reduced glutathione

DHR:

dihydrorhodamine 123

GSSG:

oxidized glutathione

EPR:

electron paramagnetic resonance

MIC(s):

minimal inhibitory concentration(s)

PS:

parental strain (6chr+)

TM(s):

CrVI-tolerant mutant(s)

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Authors and Affiliations

  1. Department of General and Environmental Microbiology, Faculty of Science, University of Pécs, Pécs, Hungary

    Zs. Koósz, Z. Gazdag, J. Antal & M. Pesti

  2. Department of Genetics and Molecular Biology, Faculty of Science, University of Debrecen, Debrecen, Hungary

    I. Miklós & Z. Benkő

  3. Institute of Biophysics, Faculty of Medicine, University of Pécs, Pécs, Hungary

    J. Belágyi

  4. Medical Genetics and Child Development, Faculty of Medicine, University of Pécs, Pécs, Hungary

    B. Meleg

Authors
  1. Zs. Koósz
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  2. Z. Gazdag
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  3. I. Miklós
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  4. Z. Benkő
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  5. J. Belágyi
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  6. J. Antal
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  7. B. Meleg
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  8. M. Pesti
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Correspondence to M. Pesti.

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Koósz, Z., Gazdag, Z., Miklós, I. et al. Effects of decreased specific glutathione reductase activity in a chromate-tolerant mutant of Schizosaccharomyces pombe . Folia Microbiol 53, 308–314 (2008). https://doi.org/10.1007/s12223-008-0048-4

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  • DOI: https://doi.org/10.1007/s12223-008-0048-4

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