Abstract
Stable chromium(VI)-sensitive and -tolerant mutants were obtained by induced mutagenesis ofSchizosaccharomyces pombe lysine and leucine auxotrophic heterothallic strains6chr + and9chr +. Eleven of them were selected for further studies. Fast transport of51CrO4 2− was detected in a representative sensitive mutant,chr-51S, while the tolerant mutantchr1-66T and the parental strain6chr + exhibited significantly lower51CrO4 2− uptake. The segregation of tetrads of three selected CrVI-tolerant mutants,chr1-66T, chr1-14T andchr2-04T, strongly indicated that tolerance was determined by single mutations. Random spore analysis proved that the mutations ofchr1-66T andchr1-14T were allelic and the mutation of mutantchr2-04T was not allelic with the mutation ofchr1-66T. Recombinants carrying theura4D18 selective marker were created for transformation experiments. Two of them (chr1-661T andchr2-046T) can be used to clone and identify the genes responsible for their CrVI tolerance phenotype.
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This work was supported by theMinistry of Education of the Hungarian Republic, grants OTKA T34157 SLO-19/2000 and NKFP 3/50/2001.
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Czakó-Vér, K., Koósz, Z., Antal, J. et al. Characterization of chromate-sensitive and-tolerant mutants ofSchizosaccharomyces pombe . Folia Microbiol 49, 31–36 (2004). https://doi.org/10.1007/BF02931642
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DOI: https://doi.org/10.1007/BF02931642