Abstract
This study evaluated the changes in sugar metabolism and fruit quality of different pear cultivars during cold storage using seven major commercial pear cultivars belonging to different Pyrus species, such as P. bretschneideri Rehd. (“Huangguan,” “Yali”), P. pyrifolia Nakai. (“Wonhwang,” “Hosui”), P. ussuriensis Maxim. (“Jingbai,” “Nanguo”), and P. communis L. (“Bartlett”). The firmness, respiration rate, titratable acidity, total soluble solids, sugar content, and enzyme activity of the seven pear cultivars were investigated. SPSS was used for analyzing the significance of different indexes. Results showed that fructose was the dominant sugar, accounting for > 60% of total sugars, followed by glucose and sucrose. The respiration peak of almost all cultivars appeared within 60 days. The levels of fructose, glucose, sucrose, and total soluble solids increased within 90 days and then generally decreased. Acid invertase showed the highest activity among all pear cultivars, followed by neutral invertase, sucrose synthetase, and sucrose phosphate synthetase during storage.
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References
Teng YW (2017) Advances in the research on phylogeny of the genus Pyrus and the origin of pear cultivars native to East Asia. J Fruit Sci 34:370–378 (in Chinese)
Gürel S, Başar H (2014) The comparison of elemental composition of Deveci and Santa Maria pear varieties. Turk J Agric Nat Sci 1:1970–1976
Manzoor M, Anwar F, Bhatti IA et al (2013) Variation of phenolics and antioxidant activity between peel and pulp parts of pear (Pyrus Communis L.) fruit. Pak J Bot 45:1521–1525
Pasquariello MS, Rega P, Migliozzi T et al (2013) Effect of cold storage and shelf life on physiological and quality traits of early ripening pear cultivars. Sci Hortic 162:341–350
Zhang HP, Wu JY, Qin GH et al (2014) The role of sucrose-metabolizing enzymes in pear fruit that differ in sucrose accumulation. Acta Physiol Plant 36:71–77
Liu D, Ni J, Wu R et al (2013) High temperature alters sorbitol metabolism in Pyrus pyrifolia leaves and fruit flesh during late stages of fruit enlargement. J Am Soc Hortic Sci 138:443–451
Ruan YL (2012) Signaling role of sucrose metabolism in development. Mol Plant 5:763–765
Liu YH, Offler CE, Ruan YL (2013) Regulation of fruit and seed response to heat and drought by sugars as nutrients and signals. Front Plant Sci 4:282
Kou XH, Li YF, Zhang Y et al (2018) Gene expression and activity of enzymes involved in sugar metabolism and accumulation during “Huangguan” and “Yali” pear fruit development. Trans Tianjin Univ 24:101–110
Verma AK, Upadhyay SK, Verma PC et al (2011) Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biol 13:325–332
Yao GF, Zhang SL, Cao YF et al (2010) Characteristics of components and contents of soluble sugars in pear fruits from different species. Scientia Agricultura Sinica 43:4229–4237
Chen J, Wang Z, Wu J et al (2007) Chemical compositional characterization of eight pear cultivars grown in China. Food Chem 104:268–275
Chen JL, Yan SJ, Feng ZS et al (2006) Changes in the volatile compounds and chemical and physical properties of Yali pear (Pyrus bertschneideri Reld) during storage. Food Chem 97:248–255
Tanase K, Yamaki S (2000) Sucrose synthase isozymes related to sucrose accumulation during fruit development of Japanese pear (Pyrus pyrifolia Nakai). J Jpn Soc Hortic Sci 69:671–676
Ticha A, Salejda AM, Hyspler R et al (2015) Sugar composition of apple cultivars and its relationship to sensory evaluation. ŻYWNOŚĆ Nauka Technologia Jakość 4:137–150
Ruan YL, Jin Y, Yang YJ et al (2010) Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Mol Plant 3:942–955
Moriguchi T, Abe K, Sanada T et al (1992) Levels and role of sucrose synthase, sucrose-phosphate synthase, and acid invertase in sucrose accumulation in fruit of Asian pear. J Am Soc Hortic Sci 117:274–278
Yamaki S (2010) Metabolism and accumulation of sugars translocated to fruit and their regulation. J Jpn Soc Hortic Sci 79:1–15
Li M, Feng F, Cheng L (2012) Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development. PLoS ONE 7:e33055
Basson C, Groenewald J, Kossmann J et al (2010) Sugar and acid-related quality attributes and enzyme activities in strawberry fruits: invertase is the main sucrose hydrolysing enzyme. Food Chem 121:1156–1162
Zhang X, Dou M, Yao Y et al (2011) Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)). Afr J Biotech 10:2716–2723
Verma AK, Upadhyay SK, Srivastava MK et al (2011) Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane. Acta Physiol Plant 33:1749–1757
Li W, Shao Y, Chen W et al (2011) The effects of harvest maturity on storage quality and sucrose-metabolizing enzymes during banana ripening. Food Bioprocess Technol 4:1273–1280
Nascimento JR, Cordenunsi BR, Lajolo FM (1997) Partial purification and characterization of sucrose phosphate synthase from preclimacteric and climacteric bananas. J Agric Food Chem 45:1103–1107
Cao S, Yang Z, Zheng Y (2013) Sugar metabolism in relation to chilling tolerance of loquat fruit. Food Chem 136:139–143
Coleman HD, Yan J, Mansfield SD (2009) Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure. Proc Natl Acad Sci 106:13118–13123
Suzuki A, Kanayama Y, Yamaki S (1996) Occurrence of two sucrose synthase isozymes during maturation of Japanese pear fruit. J Am Soc Hortic Sci 121:943–947
Guo J, Jermyn WA, Turnbull MH (2002) Carbon assimilation, partitioning and export in mature cladophylls of two asparagus (Asparagus officinalis) cultivars with contrasting yield. Physiol Plant 115:362–369
Sturm A, Tang GQ (1999) The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci 4:401–407
Singh SP, Singh Z, Swinny EE (2009) Sugars and organic acids in Japanese plums (Prunus salicina Lindell) as influenced by maturation, harvest date, storage temperature and period. Int J Food Sci Technol 44:1973–1982
Pareek S, Yahia EM, Pareek OP et al (2011) Postharvest physiology and technology of Annona fruits. Food Res Int 44:1741–1751
Zaharah SS, Singh Z, Symons GM et al (2012) Role of brassinosteroids, ethylene, abscisic acid, and indole-3-acetic acid in mango fruit ripening. J Plant Growth Regul 31:363–372
Bhat MY, Ahsan H, Banday FA et al (2012) Effect of harvest dates, preharvest calcium sprays and storage period on physico-chemical characteristics of pear cv. Bartlett. J Agri Res Dev 2:101–106
Kou XH, Jiang BL, Zhang Y et al (2016) The regulation of sugar metabolism in Huangguan pears (Pyrus pyrifolia Nakai) with edible coatings of calcium or Pullulan during cold storage. Korean J Hortic Sci Technol 34:898–912
Kou XH, Wang S, Zhang Y et al (2014) Effects of chitosan and calcium chloride treatments on malic acid-metabolizing enzymes and the related gene expression in post-harvest pear cv. ‘Huangguan’. Sci Hortic 165:252–259
Verma AK, Singh SB, Agarwal AK et al (2012) Influence of postharvest storage temperature, time, and invertase enzyme activity on sucrose and weight loss in sugarcane. Postharvest Biol Technol 73:14–21
Solomon S (2009) Post-harvest deterioration of sugarcane. Sugar Tech 11:109–123
Acknowledgements
This study was supported by a key project in the National Science and Technology Pillar Program during the 11th 5-year plan period (No. 2006BAD22B01), the National Natural Science Foundation of China (No. 31470091), and Innovation Team of Tianjin Forestry and Pomology Research System (No. ITTFPRS2018009). We are very grateful to Dr. Shijie Yan (Tianjin Agricultural University) for providing the cold room.
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Zhao, Y., Geng, J., Zhang, Y. et al. Changes in Sugar Metabolism and Fruit Quality of Different Pear Cultivars During Cold Storage. Trans. Tianjin Univ. 25, 389–399 (2019). https://doi.org/10.1007/s12209-018-0184-0
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DOI: https://doi.org/10.1007/s12209-018-0184-0