Abstract
Since amino acids are the main intermediates converted from protein and they produce ammonia which affects on the efficiency of anaerobic digestion, the effect of ammonia on anaerobic degradation of the amino acids should be evaluated. As the initial NH4 +-N concentrations increased from 0 to 5,000 mg NH4 +-N/L, deamination of the amino acids decreased from 100 to 55% implying that deamination was inhibited by the ammonia toxicity. Although the initial NH4 +-N was absent, the produced ammonia from the amino acids inhibited methane production. VFAs produced through deamination accumulated and were not converted to methane due to the ammonia toxicity. Among all of the amino acids, only β-alanine was very sensitive to ammonia inhibition. Conclusively, concentration of ammonia both in wastewater and to be produced from amino acids should be investigated for better anaerobic digestion of wastewater having a high content of protein such as hydrolysed wastewater sludge.
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References
American Public Health Association (APHA) (2005). The American WaterWorks Association (AWWA), and the Water Environment Federation (WEF), Solids, Standard Methods for the Examination of Water and Wastewater, 21st Ed., APHA, Washington, DC, 2-55–2-60.
Appels, L., Baeyens, J., Degreve, J., and Dewil, R. (2008). “Principles and potential of the anaerobic digestion of waste-activated sludge.” Progress in Energy and Combustion Science, Vol. 34, No. 6, pp. 755–781, DOI: 10.1016/j.pecs.2008.06.002.
Ato, M., Ishii, M., and Igarashi, W. (2014). “Enrichment of amino acidoxidizing, acetate-reducing bacteria.” Journal of Bioscience and Bioengineering, In press, DOI: 10.1016/j.jbiosc2014.12.003.
Baena, S., Fardeau, M. L., Labat, M., Ollivier, B., Garcia, J. L., and Patel, B. K. (1998). “Desulfovibrioaminophilus sp. nov., a Novel Amino Acid Degrading and Sulfate Reducing Bacterium from an Anaerobic Dairy Wastewater Lagoon.” Systematic and Applied Microbiology, Vol. 21, No. 4, pp. 498–504, DOI: 10.1016/S0723-2020(98)80061-1.
Barker, H. A. (1981). “Amino acid degradation by anaerobic bacteria.” Annual Review of Biochemistry, Vol. 50, No. 7, pp. 23–40, DOI: 10.1146/annurev.bi.50.070181.000323.
Calli, B., Mertoglu, B., Inanc, B., and Yenigun, O. (2005). “Effects of high free ammonia concentration on the performances of anaerobic bioreactors.” Process Biochemistry, Vol. 40, Nos. 3–4, pp. 1285–1292, DOI: 10.1016/j.procbio.2004.05.008.
Chen, Y., Cheng, J. J., and Creamer, K. S. (2008). “Inhibition of anaerobic digestion process: A review.” Bioresourece Technology, Vol. 100, No. 10, pp. 4044–4064, DOI: 10.1016/j.biortech.2007.01.057.
Chi, Y., Li, Y., Fei, X., Wang, S., and Yuan, H. (2011). “Enhancement of thermophilic anaerobic digestion of thickened waste activated sludge by combined microwave and alkaline pretreatment.” Journal of Environmental Science, Vol. 23, No. 8, pp. 1256–1265, DOI: 10.1016/S1001-0742(10)60561-X.
Duan, N., Dong, B., Wu, B., and Dai, X. (2012). “High-solid anaerobic digestion of sewage sludge under mesophilic conditions: Feasibility study.” Bioresource Technology, Vol. 104. No. 1, pp. 150–156, DOI: 10.1016/j.biortech.2011.10.090.
Kim, M., Ahn, Y., and Speece, R. E. (2002). “Comparative process stability and efficiency of anaerobic digestion; Mesophilic vs. thermophilic.” Water Research, Vol. 36, No. 17, pp. 4369–4385, DOI: 10.1016/S0043-1354(02)00147-1.
Koster, I. W. and Lettinga, G. (1998). “Anaerobic digestion at extreme ammonia concentrations.” Biological Wastes, Vol. 25, No. 1, pp. 51–59, DOI: 10.1016/0269-7483(88)90127-9.
Liang, L. Y., Zheng, Y. G., and Shen, Y. C. (2008). “Optimization of beta Alanine production from beta-aminopropionitrile be resting cells of rhodococcus sp. G20 in a bubble column reactor using response surface methodology.” Process Biochemistry, Vol. 43, No. 7, pp. 758–764, DOI: 10.1016/j.procbio.2008.03.002.
Liu, H., Wang, J., Liu, X., Fe, B., Chen, J., and Yu, H. Q. (2012). “Acidogenic fermentation of proteinaceous sewage sludge: Effect of pH.” Water Research, Vol. 46, No. 3, pp. 799–807, DOI: 10.1016/j.watres.2011.11.047.
Mcmurry, J. (2011). Organic chemistry (8 th international edition), Cengage Learning, UK.
Mottet, A., Steyer, J. P., Seleris, S., Vedrenne, F., Chauzy, J., and Carrere, H. (2009). “Kinetics of thermophilic batch anaerobic digestion of thermal hydrolysed waste activated sludge.” Biochemical Engineering Journal, Vol. 46, No. 2, pp. 169–175, DOI: 10.1016/j.bej.2008.05.003.
Nasr, N., Elbeshbishy, E., Hafes, H., Nakhla, G., and Naggar, M. H. E. (2012). “Comparative assessment of single-stage and two-stage anaerobic digestion for the treatment of thin stillage.” Bioresource Technology, Vol. 111, No. 5, pp. 122–126, DOI: 10.1016/j.biortech.2012.02.019.
Orlygsson, J., Houwen, F. P., and Svensson, B. H. (1994). “Influence of hydrogenothrophic methane formation on the thermophilic anaerobic degradation of protein and amino acids.” Microbiology Ecology, Vol. 13, No. 4, pp. 327–331, DOI: 10.1111/j.1574-6941.1994.tb00079.x.
Park, J., Park, S., and Kim, M. (2014). “Anaerobic degradation of amino acids generated from the hydrolysis of sewage sludge.” Environmental Technology, Vol. 35, No. 9, pp. 1133–1139, DOI: 10.1080/09593330.2013.863951.
Park, S., Park, J., Na, H., and Kim, M. (2012). “Estimation of influencing factors for efficient anaerobic digestion of thermally-hydrolyzed sewage sludge.” Challenges in Environmental Science & Engineering (CESE), The 5th CESE Conference, Australia Melbourne.
Ramsay, I. R. and Pullammanappallil, P. C. (2001). “Protein degradation during anaerobic wastewater treatment: derivation of stoichiometry.” Biodegradation, Vol. 12, No. 4, pp. 247–257, DOI: 10.1023/A:1013116728817.
Speece, R. E. (2008). Anaerobic biotechnology and odor/corrosion control, Archae Press, Nashville, TN.
Su, H., He, P., Yu, G. I., and Shao, L. (2011). “Effect of ultrasonic pretreatment on anaerobic digestion and its sludge dewaterability.” Journal of Environmental Science, Vol. 23, No. 9, pp. 1472–1478, DOI: 10.1016/S1001-0742(10)60618-3.
Tampio, E., Ervasti, S., Paavola, T., Heaven, S., Banks, C., and Rintala, J. (2014). “Anaerobic digestion of autoclaved and untreated food waste.” Waste Management, Vol. 34, No. 2, pp. 370–377, DOI: 10.1016/j.wasman.2013.10.024.
Wagner, A. O., Hohlbrugger, P., Lins, P., and Illmer, P. (2011). “Effects of different nitrogen sources on the biogas production — a lab-scale investigation.” Microbiol Res., Vol. 167, No. 10, pp. 630–636, DOI: 10.1016/j.micres.2011.11.007.
Wittmann, C., Zeng, A., and Deckwer, W. (1995). “Growth inhibition by ammonia and use of a pH-controlled feeding strategy for the effective cultivation of Mycobacterium chlorophenollcum.” Applied Microbiology and Biotechnology, Vol. 44, No. 3, pp. 519–525, DOI: 10.1007/BF00169954.
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Park, S., Kim, M. Effect of ammonia on anaerobic degradation of amino acids. KSCE J Civ Eng 20, 129–136 (2016). https://doi.org/10.1007/s12205-015-0240-4
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DOI: https://doi.org/10.1007/s12205-015-0240-4