Abstract
With global warming, heat stress has become a primary factor that compromises the health and milk quality of dairy cows. Here, we investigated the function and underlying regulatory mechanism of miR-27a-3p in bovine mammary epithelial cells (BMECs) under heat-stress conditions. The current study showed that miR-27a-3p could prevent heat stress-induced BMEC oxidative stress and mitochondrial damage by regulating the balance between mitochondrial fission and fusion processes. Importantly, we found that miR-27a-3p could increase cell proliferation under heat stress conditions by regulating the MEK/ERK pathway and cyclin D1/E1. Interestingly, miR-27a-3p is also involved in the regulation of milk protein synthesis-related protein expression, such as CSN2 and ELF5. Inhibition of the MEK/ERK signaling pathway by AZD6244 blocked the regulatory function of miR-27a-3p in cell proliferation and milk protein synthesis in BMECs under heat stress conditions. Our findings demonstrated that miR-27a-3p protects BMECs from heat stress-induced oxidative stress and mitochondrial damage through the MEK/ERK pathway, thereby promoting BMECs proliferation and lactation in dairy cows.
Graphical Abstract
The potential regulatory mechanism of miR-27a-3p in attenuating heat stress-induced apoptosis and lactation defect in BMECs.
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All data will be made available upon reasonable request by emailing the corresponding author.
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We thank everyone for their support in this study.
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This research was funded by the National Natural Science Foundation of China (32002169, 32102525).
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Conceptualization, K.C. and H.W.; methodology, Y.W.; software, Y.W.; validation, J.W., Q.D. and F.Z.; formal analysis, S.X.; investigation, X.Y.; resources, J.Z.; data curation, K.C.; writing-original draft writing-review and editing, K.C.; visualization, H.W.; supervision, K.C.; project administration, J.Z.; funding acquisition, K.C. All authors have read and agreed to the published version of the manuscript.”
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Yue Wang and Jie Wu are co-first authors.
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Wang, Y., Wu, J., Xia, SW. et al. miR-27a-3p relieves heat stress-induced mitochondrial damage and aberrant milk protein synthesis through MEK/ERK pathway in BMECs. Cell Stress and Chaperones 28, 265–274 (2023). https://doi.org/10.1007/s12192-023-01334-z
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DOI: https://doi.org/10.1007/s12192-023-01334-z