Abstract
Intramuscular fat (IMF) is a critical factor in beef quality. IMF is mainly distributed between muscle fibres and its accumulation can affect the marbling and meat quality of beef. IMF formation and deposition is a complex process and in recent years a group of non-coding RNAs (ncRNAs), known as circRNAs, have been discovered to play an important role in regulating intramuscular fat deposition. CircRNAs form a covalent loop structure after reverse splicing of precursor mRNAs. They can act by adsorbing miRNAs, thereby reducing their repressive effects on downstream target genes. Based on high-throughput sequencing of circRNAs in intramuscular fat of Qinchuan and Japanese black cattle, we identified a novel circSSBP2 that is differentially expressed between the two species and associated with adipogenesis. We show that circSSBP2 knockdown promotes bovine intramuscular preadipocyte proliferation, whereas overexpression inhibits bovine intramuscular preadipocyte proliferation. We also show that circSSBP2 can act as a molecular sponge for miR-2400 and that miR-2400 overexpression promotes bovine intramuscular preadipocyte proliferation. Furthermore, N-myc downstream-regulated gene 1 (NDRG1) was identified as a direct target gene of miR-2400, and NDRG1 interference promoted the proliferation of bovine intramuscular preadipocytes. In conclusion, our results suggest that circSSBP2 inhibits the proliferation of bovine intramuscular preadipocytes by regulating the miR-2400/NDRG1 axis.
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Acknowledgements
This study was funded by the Key Special Project for the Integration of Two Chains of Livestock and Poultry Breeding in Shaanxi Province (2022GD-TSLD-46- 0102), the National Key Research and Development Program of China (2018YFD0501700), the National Natural Science Foundation of China (31972994), the National Beef Cattle and Yak Industry Technology System (CARS-37), and the Fundamental Research Funds for the Central Universities(SWU-KQ24030).
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Yanqing Zhao: Conceptualization, Investigation, Methodology, Validation, Writing - original draft, and Writing - review &editing. Wenzhen Zhang: Conceptualization, Data curation, and Writing - review &editing. Sayed Haidar Abbas Raza and Xiaopeng Qu: Conceptualization, Validation Data curation, and Writing - review &editing. Jiahan Deng: Jing Ma, Bandar Hamad Aloufi: Investigation and Formal analysis. Juze Wang: Zhimei Yang: Investigation and Validation. Linsen Zan: Conceptualization, Project administration, Resources, and Funding acquisition.
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Zhao, Y., Zhang, W., Raza, S.H.A. et al. CircSSBP2 acts as a MiR-2400 sponge to promote intramuscular preadipocyte proliferation by regulating NDRG1. Mol Genet Genomics 299, 48 (2024). https://doi.org/10.1007/s00438-024-02138-1
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DOI: https://doi.org/10.1007/s00438-024-02138-1