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Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population

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Cell Stress and Chaperones Aims and scope

Abstract

Atherosclerosis is a chronic inflammatory and autoimmune disease. Candidate molecules/autoantigens include heat shock proteins (HSPs); Hsp70 (HSPA1A) is one of the best studied HSPs. Various studies have shown a correlation between extracellular Hsp70 (eHsp70) and anti-Hsp70/anti-Hsp60 antibody concentration and development of atherosclerosis. A random sample of 456 people aged 40–60 (218 males, 234 females) was studied to investigate the prevalence of traditional vascular risk factors and eHsp70 and anti-Hsp70/anti-Hsp60 antibodies levels, according to the risk of vascular disease. Task Force Chart was applied for classification. Subjects were divided into three groups: G0 (with no vascular risk factor or a risk lower than 5%), n = 239; G1 (moderated 10–20% risk, who do not have established disease) n = 161; and G2 (established atherosclerosis disease) n = 52. eHsp70 and anti-Hsp70 were significantly lower in the atherosclerosis group (group 2) with respect to the other groups. Disease-free people showed the highest anti-Hsp60 concentration compared with the other two groups. A correlation has not been demonstrated between the concentrations of circulating Hsp70 (HSPA1A), anti-Hsp70, and anti-Hsp60 and classical vascular risk factors and C-reactive protein. Low levels of eHsp70 and anti-Hsp70 antibodies should be considered as candidate FRV. Simultaneous decrease of eHsp70 and anti-Hsp70 antibodies would be explained by circulating immune complex formation, and both could be proposed as biomarkers for the progression of atherosclerotic disease. Levels of circulating anti-Hsp60 antibodies may constitute a marker of inflammation in atherosclerosis.

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Acknowledgements

The authors are grateful to Dr. Jose Luis Bellón Cano for valuable statistical consultations and Mrs. Maria Jesús Sánchez for their invaluable help in sample processing.

This study was supported by FIS grant 03/1308 and Fundación Mutua Madrileña.

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Authors and Affiliations

  1. Biochemistry Department, Hospital General Universitario “Gregorio Marañón”, Madrid, Spain

    Elena Dulin

  2. Experimental Medical and Surgery Unit, Hospital General Universitario “Gregorio Marañón”, Dr. Esquerdo 46, 28007, Madrid, Spain

    Pedro García-Barreno & Maria C. Guisasola

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  1. Elena Dulin
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  2. Pedro García-Barreno
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  3. Maria C. Guisasola
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Correspondence to Maria C. Guisasola.

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Dulin, E., García-Barreno, P. & Guisasola, M.C. Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population. Cell Stress and Chaperones 15, 929–937 (2010). https://doi.org/10.1007/s12192-010-0201-2

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