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Graphene Oxide Synergy with the Conjugation of DNA and Quantum Dots for the Sensitive Detection of Ochratoxin A

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Abstract

Ochratoxin A (OTA) is one of the most common toxins in food and feed, which seriously affects the health of public. Herein, a convenient biosensor for OTA detecting was built based on graphene oxide (GO) and quantum dots-aptamer (QDs-aptamer) in this investigation. The results in our study demonstrated that the constructed biosensor shows excellent sensitivity and highly selectivity for OTA. A lower limit of detection of 376.3 pM (equivalent to 0.152 ng·mL−1) was achieved in the linear range from 0.5 to 20 nM (R2 = 0.996) under optimized conditions. In addition, the constructed sensor was challenged by testing coffee and red wine samples that contained buffer solution spiked with different concentrations of OTA. Considering easy operating and superior sensitivity, it has great potential, provides a high-performance platform for OTA testing in complicated food sample, and also affords a pathway for analysis of other aptamer-specific molecules.

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Funding

We are greatly appreciating Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (PPZY2015B146), Wuxi Science and Technology Development Fund (N20192044), and 2018 Innovative Research Team of Jiangsu Province for the support of this research work.

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Authors and Affiliations

  1. Guangzhou Yuexiu District Center for Disease Control and Prevention, Guangzhou, 510055, China

    Qiongxia Li

  2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Yu Kang & Zhaoqi Yang

  3. School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, China

    Shaoxian Yin, Yue Qian, Yanfei Cai & Zhaoqi Yang

Authors
  1. Qiongxia Li
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  2. Yu Kang
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  3. Shaoxian Yin
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  4. Yue Qian
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  5. Yanfei Cai
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  6. Zhaoqi Yang
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Correspondence to Zhaoqi Yang.

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Qiongxia Li declares that she has no conflict of interest. Yu Kang declares that he has no conflict of interest, Shaoxian Yin declares that he has no conflict of interest. Yue Qian declares that she has no conflict of interest, Yanfei Cai declares that she has no conflict of interest. Zhaoqi Yang declares that she has no conflict of interest.

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Qiongxia Li and Yu Kang contributed equally to this work.

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Li, Q., Kang, Y., Yin, S. et al. Graphene Oxide Synergy with the Conjugation of DNA and Quantum Dots for the Sensitive Detection of Ochratoxin A. Food Anal. Methods 15, 440–447 (2022). https://doi.org/10.1007/s12161-021-02135-9

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  • DOI: https://doi.org/10.1007/s12161-021-02135-9

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